miR-122对乙型肝炎病毒抗原表达的影响

发布时间：2018-04-30 23:34

本文选题：微RNAs/治疗应用 + 肝炎e抗原　；参考：《浙江大学学报(医学版)》2011年06期

【摘要】：目的:探讨miR-122对乙型肝炎病毒HBsAg、HBeAg表达的影响。方法:设计合成2条miR-122的反义寡核苷酸(anti-miR-122,LNA-antimiR-122)、hsa-miR-122以及阴性对照anti-GFP,脂质体介导转染HepG2.2.15细胞。取细胞转染24 h、48 h后的培养液,时间分辨荧光免疫法检测anti-miR-122组、LNA-antimiR-122组、hsa-miR-122组以及anti-GFP组HBsAg和HBeAg的表达,定量PCR检测各组HBV DNA的表达。转染48 h后,提取细胞内总RNA,Taqman技术实时荧光定量PCR检测各组miR-122的表达。结果:①转染48 h后anti-miR-122组、LNA-antimiR-122组miR-122表达明显受抑制,低于阴性对照组(P0.001)。hsa-miR-122组miR-122水平较阴性对照组升高(P0.001)。②hsa-miR-122组HBsAg、HBeAg表达均低于阴性对照组(P0.001);anti-miR-122组、LNA-antimiR-122组HBsAg、HBeAg表达均高于阴性对照组(P0.001)。③转染24 h与48 h后各组HBV DNA表达与阴性对照组比较无统计学意义(P0.05)。结论:miR-122可调节乙型肝炎病毒抗原表达。
[Abstract]:Objective: to investigate the effect of miR-122 on the expression of hepatitis B virus HBsAg HBeAg. Methods: two antisense oligodeoxynucleotides (miR-122) anti-miR-122 (LNA-antimiR-122) and negative control (anti-GFP) were designed and synthesized and transfected into HepG2.2.15 cells by liposome. The expression of LNA-antimiR-122 HBsAg and HBeAg in anti-miR-122 group and anti-GFP group were detected by time-resolved fluorescence immunoassay. The expression of HBV DNA in each group was detected by quantitative PCR. After 48 hours of transfection, the miR-122 expression in each group was detected by real-time fluorescent quantitative PCR with Taqman technique. Results the expression of LNA-antimiR-122 miR-122 was inhibited in anti-miR-122 group 48 h after transfection. Compared with the negative control group, the level of miR-122 in the HBeAg group was significantly higher than that in the negative control group (P 0.001). 2hsa-miR-122 group was significantly lower than that in the negative control group (P 0.001) anti-miR-122 group. The expression of HBsAg HBeAg in the LNA-antimiR-122 group was higher than that in the negative control group at 24 h and 48 h after transfection, and the expression of HBsAg HBeAg in all groups was higher than that in the negative control group at 24 h and 48 h after transfection. There was no significant difference between the two groups (P 0.05). Conclusion: miR-122 can regulate the expression of hepatitis B virus antigen.
【作者单位】：浙江大学医学院附属第一医院传染病诊治国家重点实验室;浙江大学免疫研究所;
【基金】：十二五传染病重大专项(2012ZX10002-007) 国家重点基础研究项目(2007CB512905,2007CB513001) 浙江省自然科学基金项目(Y207534,Y2080428)
【分类号】：R512.62

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