超顺磁性氧化铁标记骨髓间充质干细胞诱导分化为神经细胞的体外研究

发布时间：2018-05-02 05:29

本文选题：超顺磁性氧化铁 + 骨髓间充质干细胞　；参考：《山西医科大学》2010年硕士论文

【摘要】： 背景：一些研究者利用超顺磁性氧化铁对骨髓间充质干细胞进行标记,并利用MRI技术对标记细胞肝内、肾内移植后进行初步的活体示踪,但是,对于超顺磁性氧化铁标记骨髓间充质干细胞后,对其存活、增殖以及分化是否有影响研究较少。目的：观察超顺磁性氧化铁标记骨髓间充质干细胞后,对其存活、增殖以及向神经细胞的分化是否有影响。方法：使用超顺磁性氧化铁标记大鼠骨髓间充质干细胞,采用普鲁士蓝染色法鉴定其标记率、台盘蓝染色法检测细胞活力、MTT法检测标记干细胞的细胞增值活力、以1 mmol／Lβ-巯基乙醇及无血清DMEM培养液体外诱导标记细胞向神经细胞分化并用免疫组织化学鉴定诱导后细胞、再次使用普鲁士蓝染色法鉴定神经细胞内的铁颗粒。结果与结论：普鲁士蓝染色对干细胞的标记率接近100%,台盘蓝染色显示标记细胞的存活率不受影响；MTT法检测发现标记干细胞的增殖活力与未标记干细胞相比差异无显著性意义(P0.05)；以β-巯基乙醇诱导后大部分骨髓间充质干细胞分化为神经元样细胞、免疫组织化学阳性,再次普鲁士蓝染色显示铁颗粒位于神经细胞的细胞浆内。提示超顺磁性氧化铁标记骨髓间充质干细胞后,对于干细胞的存活、增殖以及向神经细胞的分化无影响。
[Abstract]:Background: some researchers used superparamagnetic ferric oxide to label bone marrow mesenchymal stem cells and MRI technique was used to trace the labeled cells in liver and kidney, but, There are few studies on the survival, proliferation and differentiation of bone marrow mesenchymal stem cells labeled with superparamagnetic iron oxide. Aim: to investigate the effects of superparamagnetic ferric oxide on the survival, proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs). Methods: superparamagnetic ferric oxide was used to label rat bone marrow mesenchymal stem cells (BMSCs). Prussian blue staining was used to identify the labeling rate, and table-blue staining was used to detect cell viability. MTT assay was used to detect the cell proliferation activity of labeled stem cells. The labeled cells were induced to differentiate into neural cells with 1 mmol/L 尾 -mercaptoethanol and serum-free DMEM in vitro. The induced cells were identified by immunohistochemistry, and the iron granules in the neurons were identified by Prussian blue staining. Results and conclusion: the labeling rate of stem cells by Prussian blue staining was close to 100. The viability of labeled stem cells was not affected by plate blue staining. The proliferative activity of labeled stem cells was not different from that of unlabeled stem cells by MTT assay. Most bone marrow mesenchymal stem cells differentiated into neuron-like cells after induced by 尾 -mercaptoethanol. Immunohistochemical staining showed that iron granules were located in the cytoplasm of nerve cells. These results suggest that superparamagnetic ferric oxide labeled bone marrow mesenchymal stem cells has no effect on the survival, proliferation and differentiation of stem cells.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R329

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