福州市性病门诊人群生殖道沙眼衣原体的分子流行病学研究

发布时间：2018-05-02 07:48

本文选题：生殖道沙眼衣原体 + 基因分型　；参考：《中国人兽共患病学报》2015年10期

【摘要】：目的探讨福州市性病门诊人群泌尿生殖道沙眼衣原体的基因型分布状况。方法收集2013-2014年临床疑似生殖道沙眼衣原体感染者标本2 019份,用实时荧光定量PCR检测沙眼衣原体,阳性标本用套式PCR扩增Omp1区并进行测序分析。测序结果上传至BLAST网站查找序列的相似性、构建系统树确定基因型。结果检测沙眼衣原体阳性标本86份,成功分型的有83份,1株为混合感染。测得8个基因型,分别为:F型(36.59%)、E型(24.39%)、D型(12.20%)、G型(9.76%)、J型(9.76%)、H型(3.66%)、K型(2.44%)、B型(1.22%)。计算不同基因型间Omp1基因的核苷酸的同义突变率(dS)和非同义突变率(dN),发现仅G型的dN/dS值大于1,其他型均小于1。结论 F型和E型是福州市性病门诊人群泌尿生殖道沙眼衣原体的主要基因型。同义替代是Omp1基因在进化过程中的主要变异。
[Abstract]:Objective to investigate the genotype distribution of Chlamydia trachomatis in urogenital tract in STD outpatients in Fuzhou. Methods 2019 samples of suspected genital chlamydia trachomatis infection from 2013 to 2014 were collected and detected by real-time fluorescence quantitative PCR. The positive samples were amplified by nested PCR and sequenced. The sequencing results were uploaded to the BLAST website to find the similarity of the sequences, and the system tree was constructed to determine the genotypes. Results 86 chlamydia trachomatis positive specimens were detected and 83 strains of chlamydia trachomatis were successfully classified as mixed infection. Eight genotypes were determined, respectively, which were: 1 / F / 36.59C / T = 24.39 / D / 12.20 / T / 9.76C / 9.76 / 9.76 / 3.66 / 3.66 / K / 2.44 / 2 / 2.22 / 2 / 2, respectively. The nucleotide synonymous mutation rate (DS) and non-synonymous mutation rate (DS) of Omp1 gene among different genotypes were calculated. It was found that only the dN/dS value of G genotype was greater than 1, while that of other genotypes was less than 1. Conclusion F and E are the main genotypes of Chlamydia trachomatis in urogenital tract. Synonymous substitution is the main variation in the evolution of Omp1 gene.
【作者单位】：福建省疾病预防控制中心;
【分类号】：R374

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