Slc25a3基因在低硒大鼠心肌细胞内的表达

发布时间：2018-05-05 16:32

本文选题：低硒 + 大鼠　；参考：《黑龙江医药科学》2015年06期

【摘要】：目的:探讨低硒状态下SD大鼠心肌细胞内Slc25a3基因的表达情况。方法:将24只正常成年雄性SD大鼠随机等分为低硒组和正常组。两组大鼠分别给予低硒饮食及正常饮食。饲养4周后,进行动物模型鉴定实验。采用荧光法检测两组大鼠主要脏器的硒含量、采用谷胱甘肽过氧化物酶试剂盒检测肝脏中谷胱甘肽过氧化物酶1(GPx-1)的活性、采用Realtime PCR方法检测GPx-1的mRNA水平的相对表达、采用丙二醛(MDA)测定试剂盒和超氧化物歧化酶(SOD)测试盒检测两组动物模型主要脏器中MDA含量和SOD活性;8周后,脊髓离断处死全部大鼠,取心脏组织进行下一步实验。结果:低硒组大鼠体内主要脏器硒含量均明显低于对照组,氧化应激水平明显高于对照组,心肌细胞内Slc25a3基因相对表达量明显高于对照组。结论:硒元素缺乏可以导致大鼠心肌细胞内Slc25a3基因相对表达量明显升高。
[Abstract]:Aim: to investigate the expression of Slc25a3 gene in myocardial cells of SD rats under selenium deficiency. Methods: 24 normal adult male SD rats were randomly divided into low selenium group and normal group. Two groups of rats were given selenium deficiency diet and normal diet. After feeding for 4 weeks, the animal model identification experiment was carried out. The content of selenium in the main organs of the two groups was detected by fluorescence method, the activity of glutathione peroxidase (Glutathione peroxidase 1GPx-1) in liver was detected by glutathione peroxidase kit, and the relative expression of mRNA level of GPx-1 was detected by Realtime PCR method. Malondialdehyde (MDA) assay kit and superoxide dismutase (SOD) kit were used to detect the content of MDA and the activity of SOD in the main organs of the two groups. After 8 weeks, all the rats were killed by spinal cord amputation, and the heart tissue was taken for the next experiment. Results: the content of selenium in the main organs of the rats in the low selenium group was significantly lower than that in the control group, the level of oxidative stress was significantly higher than that in the control group, and the relative expression of Slc25a3 gene in myocardial cells was significantly higher than that in the control group. Conclusion: selenium deficiency can significantly increase the relative expression of Slc25a3 gene in rat cardiomyocytes.
【作者单位】：佳木斯大学附属第一医院;齐齐哈尔建华医院;
【基金】：黑龙江省自然科学基金项目,编号:H201365 佳木斯大学重点项目,编号:Sz2013-004;佳木斯大学研究生科技创新项目,编号:LZZ2015_017
【分类号】：R329.2

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