苯诱发脂质过氧化代谢产物丙二醛的色谱分析方法建立和人群应用的研究

发布时间：2018-05-08 19:23

  本文选题：苯代谢物 + 丙二醛　； 参考：《郑州大学》2010年硕士论文

【摘要】： 苯是芳香族中结构最简单的化合物,作为一种良好的有机溶剂被广泛的应用于工农业生产中。长期接触苯可导致人类血液系统的损害,主要表现为血细胞减少症、贫血、再生障碍性贫血和白血病等。但苯本身并不致癌,其致癌性是通过体内代谢酶的代谢活化而产生的有毒代谢物质所致。苯的毒性代谢产物氢醌和苯醌可在骨髓中产生活性氧簇(ROS),ROS可引发脂质过氧化链式反应产生羟基过氧化代谢产物,经非酶性降解形成一些小分子的末端代谢产物,α,β-不饱和醛类化合物,如丙二醛(Malonaldehyde, MDA)和壬烯(4-hydroxy-2-nonenal, HNE)。 本文利用高效液相色谱、衍生化法等技术对苯作业工厂的工人和无苯作业工厂的工人的血浆样品和尿样中的丙二醛(Malonaldehyde, MDA)分析方法进行了系统研究。研究内容包括以下三个方面： 1.通过对样品前处理方法及色谱条件的优化,建立了一种方便快捷的分离分析人血浆样品中MDA的2-硫代巴比妥酸衍生化／高效液相色谱法,保留时间4.1 min,检测限为0.043 nmol/mL。本实验采用了较低的衍生化反应温度(80℃),克服了以往因反应温度过高而引入非样品所固有的MDA的缺点。该方法色谱峰型好,分离时间较短、灵敏度较高,大大提高了分析效率。 2.对样品前处理方法和色谱分离条件的优化选择后,建立了一种方便快捷的分离分析人血浆样品中MDA的2,4-二硝基苯肼衍生化／高效液相色谱法,保留时间25.9 min,检测限为0.098 nmol/mL。本实验只需在室温下便可快速完成衍生反应,此外,所需的pH条件也很温和,该衍生化反应对醛具有很强的专一性。 3.研究建立了一种可靠性强、灵敏度高的测定尿样中MDA的2,4-二硝基苯肼衍生化／高效液相色谱法,保留时间8.9 min,检测限为0.017 nmol/mL。该法前处理在37℃下进行,条件温和；采用“衍生化／萃取”一步法,操作简便,省时省力,适用于大批样品的快速测定。
[Abstract]:Benzene is one of the simplest aromatic compounds and is widely used in industrial and agricultural production as a good organic solvent. Long-term exposure to benzene can lead to damage to the human blood system, mainly as hematopenia, anemia, aplastic anemia and leukemia. However, benzene itself is not carcinogenic. Its carcinogenicity is caused by toxic metabolites produced by metabolic activation of metabolic enzymes in vivo. Hydroquinone and benzoquinone, the toxic metabolites of benzene, can produce reactive oxygen species in bone marrow. Ros can induce lipid peroxidation chain reaction to produce hydroxyl peroxide metabolites. The end metabolites of some small molecules, 伪, 尾 -unsaturated aldehydes, such as malondialdehyde Malonaldehyde (MDAA) and nonene 4-hydroxy-2-nonenallic, HNEE, were formed by non-enzymatic degradation. In this paper, the methods for the determination of malonaldehyde (MDA) in plasma and urine samples of workers in benzene factory and in workers without benzene were systematically studied by means of high performance liquid chromatography (HPLC) and derivatization method. The research includes the following three aspects: 1. By optimizing the sample pretreatment method and chromatographic conditions, a convenient and rapid method for the separation and analysis of MDA in human plasma by 2-thiobarbituric acid derivatization / HPLC was established. The retention time was 4.1 min and the detection limit was 0.043 nmol 路mL ~ (-1). In this experiment, a relatively low temperature of 80 鈩，

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