Anti-DNP IgE联合DNFB诱导免疫性接触性荨麻疹小鼠模型的建立

发布时间：2018-05-08 20:39

本文选题：免疫性接触性荨麻疹模型 + 小鼠　；参考：《皮肤性病诊疗学杂志》2015年01期

【摘要】：目的:利用抗-二硝基苯酚(anti-DNP)Ig E单克隆抗体和2,4-二硝基氟苯(DNFB)建立免疫性接触性荨麻疹小鼠模型。方法:将36只BALB/c小鼠随机分成3组,每组12只:造模组小鼠尾静脉注射anti-DNP Ig E单克隆抗体,24 h后在小鼠双耳涂DNFB进行激发;单纯Ig E组仅注射anti-DNP Ig E单克隆抗体,空白对照组小鼠注射生理盐水,均在24 h后在小鼠双耳涂生理盐水。观察小鼠在14 d内的风团、瘙痒、耳朵肿胀度情况,并检测血清中Ig E、IL-4、IFN-γ、组胺水平和皮肤病理。结果:造模组全部小鼠产生风团;与其它两组相比,造模组小鼠搔抓次数、耳朵肿胀度及组胺血清水平均明显升高(P值均0.01),但三组间Ig E、IL-4、IFN-γ水平无明显差异(P0.05)。结论:静脉注射anti-DNP Ig E单克隆抗体联合外用DNFB可快速、可靠地建立免疫性接触性小鼠荨麻疹模型。
[Abstract]:Objective: to establish an immune contact urticaria model in mice by using anti two nitrophenol (anti-DNP) Ig E monoclonal antibody and 2,4- two nitrofluoro benzene (DNFB). Methods: 36 BALB/c mice were randomly divided into 3 groups, 12 rats in each group were injected into the tail vein of the model mice with anti-DNP Ig E mono clone antibody, 24 h after the double ear smear DNFB in mice, and simple I. The G E group was only injected with anti-DNP Ig E monoclonal antibody, the blank control group was injected with saline after 24 h, and the mice were treated with saline in the double ears after 24 h. The mice in the air mass, itching and ear swelling were observed in the mice in 14 d, and the serum Ig E, IL-4, IFN- gamma, histamine level and Dermatopathology in the serum were detected. In the two groups, the number of scratching, the ear swelling and the histamine serum level were significantly increased (P value was 0.01), but the levels of Ig E, IL-4 and IFN- gamma were not significantly different between the three groups (P0.05). Conclusion: the intravenous anti-DNP Ig E monoclonal antibody combined with external DNFB can quickly speed up the immune contact mouse urticaria model.

【作者单位】：广州市皮肤病防治所;广东药学院;
【基金】：广东省自然科学基金(编号:S2012020011077) 广东省医学科研基金(编号:A2013543) 广州市医药卫生科技项目(编号:20131A011126)
【分类号】：R758.24;R-332

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