人乳头瘤病毒58型L1和L2壳蛋白的病毒样颗粒自组装研究

发布时间：2018-05-10 07:34

本文选题：HPV58 + L1L2　；参考：《吉林大学》2008年硕士论文

【摘要】： 本论文以人乳头瘤病毒58型(human papillomavirus type 58,HPV58)的晚期蛋白L1基因和晚期蛋白L2基因分别表达的晚期蛋白HPV58 L1和HPV58 L2作为研究对象,在体外将这两种蛋白组装成为病毒样颗粒VLP(virus like praticles)。我们将本实验室已构建的pRSETB-HPV58 L1和pRSETB-HPV58 L2整合型质粒分别转化进BL21大肠杆菌中,获得稳定表达HPV58 L1蛋白和HPV58 L2蛋白的重组菌株;然后利用IPTG诱导表达这两种蛋白;纯化这两种蛋白;并复性了HPV58 L1蛋白;然后利用这两种蛋白组装成VLP。研究表明在现有的实验条件下原核系统表达的HPV58 L1蛋白可以在体外单独组地装成VLP,而HPV58 L2蛋白则不能与HPV58 L1蛋白共同组装成VLP.。
[Abstract]:In this study, HPV58 L1 and HPV58 L2, which were expressed in late protein L1 and L2 gene of human papillomavirus 58 papillomavirus type 58 / HPV58, respectively, were used to assemble these two proteins into virus-like particles VLP(virus like praticlesus in vitro. We transformed pRSETB-HPV58 L1 and pRSETB-HPV58 L2 integrative plasmids constructed in our laboratory into BL21 Escherichia coli, and obtained the recombinant strain expressing HPV58 L1 protein and HPV58 L2 protein stably, then expressed these two proteins by IPTG induction, and purified the two proteins. HPV58 L1 protein was refolded and then assembled into VLP using these two proteins. The results showed that the HPV58 L1 protein expressed in prokaryotic system could be assembled into VLP alone in vitro, while HPV58 L2 protein could not be assembled with HPV58 L1 protein.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R373

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