SD大鼠肺气肿模型的建立及全反式维甲酸干预作用探讨

发布时间：2018-05-10 12:41

本文选题：肺气肿 + 大鼠　；参考：《中国药理学通报》2008年05期

【摘要】：目的观察不同浓度的全反式维甲酸(all-transretinoicacid,ATRA)对猪胰蛋白酶复制的大鼠肺气肿模型的治疗作用。方法建立猪胰蛋白酶(porcine pancreatic elastase,PPE)复制的大鼠肺气肿模型,将体重170~220 g的60只SD大鼠随机分为生理盐水对照组(N)、模型组(P)、小剂量ATRA治疗组(R1)、中剂量ATRA治疗组(R2)、大剂量AT-RA治疗组(R3)、棉籽油组(C)各10只。后5组向大鼠气管内滴注1U·g-1PPE,N组同样滴注等量生理盐水,饲养76d,治疗组和棉籽油组分别向大鼠腹腔内连续注入大、中、小剂量ATRA和棉籽油14 d。测量干预前后的大鼠肺体积,血气分析和病理形态学图像分析了解不同浓度ATRA对于PPE复制的大鼠肺气肿模型的治疗效果。结果R1、R2、R3组、P组、C组的大鼠肺体积明显大于N组(P0.01),C组和P组的肺体积无差异,各治疗组的肺体积较P组有差异,各治疗组之间的肺体积无差异。肺泡形态学结果显示R1、R2、R3组、P组、C组与N组比较,每个视野内的肺泡数(Na)明显减少,平均肺泡面积(MAA)增大,平均内衬间隔(MLI)较大,差异有显著性(P0.05);R1、R2组相对于P组Na增加,MAA减少,差异有统计学意义,但两组之间无差别;R3组、C组和P组的各指标无明显差别。血气分析结果P组、R1、R2、R3组和C组的二氧化碳分压(PaCO2)比N组升高,P组、C组和R3组的氧分压(PaO2)、氧饱和度(SaO2)值明显低于N组,R1、R2组的PaO2、SaO2值与N组无差异,与P组也无差异。结论中、低剂量组的ATRA对肺气肿模型的治疗在肺泡修复上明显效果,但肺功能的改善不明显;大剂量组ATRA治疗后效果不明显。
[Abstract]:Objective to observe the therapeutic effect of all-trans retinoic acid (ATRAA) at different concentrations on rat emphysema induced by porcine trypsin. Methods the rat model of emphysema induced by porcine porcine pancreatic elastase (PPE) was established. Sixty SD rats weighing 170 ~ 220g were randomly divided into normal saline control group (n = 10), model group (n = 10), low dose ATRA group (n = 10), middle dose ATRA group (n = 10), high dose AT-RA group (n = 10) and cottonseed oil group (n = 10). The rats in the latter five groups were given the same amount of normal saline intratracheal drip of 1U g-1PPEN for 76 days. The treatment group and the cottonseed oil group were continuously injected into the abdominal cavity of the rats for 14 days, middle and small doses of ATRA and cottonseed oil were respectively injected into the abdominal cavity of the rats. Lung volume, blood gas analysis and pathomorphological image analysis were measured before and after intervention to investigate the therapeutic effect of different concentrations of ATRA on rat emphysema induced by PPE. Results there was no significant difference in lung volume between P group and P group (P 0.01) and P group P (P < 0.05). There was no difference in lung volume between treatment group and P group, but there was no difference between treatment group and treatment group. The results of alveolar morphology showed that the number of alveoli in group C was significantly lower than that in group N, the mean alveolar area (MAA) was increased, and the MLI (mean lining interval) was higher in group R1, R2 and R3 than that in group N. The difference was significant (P 0. 05 and R1 / 2, P < 0. 05). The difference was statistically significant, but there was no significant difference between the two groups. The results of blood gas analysis showed that the Paco _ 2 of P group was significantly higher than that of N group. The oxygen partial pressure and oxygen saturation of P group were significantly lower than those of N group and N group, and there was no difference between P group and P group. Conclusion the treatment of emphysema model with ATRA in low dose group has obvious effect on alveolar repair, but the improvement of pulmonary function is not obvious, but the effect of ATRA in high dose group is not obvious.
【作者单位】：安徽医科大学第一附属医院老年呼吸内科安徽医科大学第一附属医院老年呼吸内科安徽医科大学第一附属医院老年呼吸内科安徽医科大学第一附属医院老年呼吸内科安徽医科大学第一附属医院老年呼吸内科
【基金】：安徽省教育厅自然科学基金资助项目(No2003KJ211)
【分类号】：R-332

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