兔多器官VX2动物模型建立的实验研究

发布时间：2018-05-11 07:52

本文选题：兔 + VX2　；参考：《泰山医学院》2010年硕士论文

【摘要】：目的改进兔VX2肝癌、肾癌、乳腺癌、脑瘤模型的制作方法,使之更为简便、省时,并且提高成瘤率。同时,探讨肿瘤模型影像学表现。方法 (1)VX2肝癌模型的制作新西兰大白兔22只,随机分成2组(每组11只),采用B超引导下经皮穿刺18G针植入VX2瘤块组织法分别接种于兔肝脏左叶(A组),兔肝脏右叶(B组);接种后1周、2周、3周、4周分别行彩超、CT和常规病理检查。 (2)VX2肾癌模型的制作新西兰大白兔22只,随机分成2组(每组11只),采用B超引导下经皮穿刺18G针植入VX2瘤块组织悬液法分别接种于兔左肾(A组)及右肾(B组);接种后1周、2周、3周、4周分别行彩超、CT和常规病理检查。 (3)VX2乳腺癌模型的制作新西兰大白兔22只,随机分成2组(每组11只),分别采用经皮穿刺18G针植入VX2瘤块组织悬液法(A组),切开局部皮肤VX2瘤块组织移植法(B组),接种于兔胸壁左侧第2乳头垫下接种后1周、2周、3周、4周分别行彩超、CT、MR和常规病理检查。 (4).VX2脑瘤模型的制作新西兰大白兔1 2只,随机分成2组(每组6只),分别采用微创定位颅骨钻孔组织块移植法(A组：取事先剪成大小约1mm3的瘤组织块1～2块植入预先打好的颅骨孔内)及微创定位颅骨钻孔瘤组织块悬液注入法(B组：取VX2肿瘤组织块悬液0.3ml注射接种入预先打好的颅骨孔内)。接种后1周、2周、3周、4周分别行CT及MR检查,观察神经系统体征变化,自然死亡后观察组织病理学改变。统计肿瘤接种成功率;观察记录术后并发症：观察肿瘤的大体表现及转移情况;观察动物自然生存情况;死亡后尸检;肿瘤组织经10%福尔马林固定后,病理检查。结果 1.兔VX2肝癌模型：22只实验兔发现瘤灶,总成瘤率72.7%(17/22),肝左叶接种组(A组)成功率90.9%(10/11),肝右叶接种组(B组)成功率为54.5%(7/11),两组接种成功率无显著差异, (P0.05)。 2.兔VX2肾癌模型：15只实验兔发现瘤灶,总接种成功率为68.2%(15/22),左肾接种组(A组)成功率为54.5%(6/11),右肾接种组(B组)成功率为81.8%(9/11),两组接种成功率无显著差异,(P0.05)。 3.兔VX2乳腺癌模型：18只实验兔发现瘤灶,总接种成功率为81.8%(18/22),组织块悬液种植法接种组(A组)成功率为90.9%(10/11),组织块移植组(B组)成功率为72.7(8/11),两组接种成功率无显著性差异(P0.05)。 4.兔VX2脑瘤模型：5只实验兔发现瘤灶,总接种成功率为41.7%(5/12),组织块移植组(A组)成功率为83.3%(5/6),组织块悬液种植组(B组)成功率为0(0/6),AB两组接种成功率有显著性差异(P0.01)。结论采用彩超引导下经皮穿刺植入组织块法建立兔VX2肝癌模型、兔VX2肾癌模型,采用组织块悬液注射法建立兔VX2乳腺癌模型,采用组织块移植法建立兔VX2脑瘤模型,使制作过程更为简便、省时：采用彩超、CT及MR平扫对瘤灶进行动态检测、评价,可更为准确、及时地观察瘤灶的发生、发展等影像学改变。
[Abstract]:Purpose The method of making rabbit VX2 liver cancer, kidney cancer, breast cancer and brain tumor model was improved to make it more simple and time saving, and to increase the rate of tumorigenesis. At the same time, the imaging findings of tumor model were discussed. Method Establishment of 22 New Zealand White Rabbit models of VX2 Hepatocellular carcinoma Each group was randomly divided into two groups (11 rats in each group), each group was inoculated with percutaneous 18G needle implantation of VX2 tumor block under the guidance of B-ultrasound in Zuo Ye group A of rabbit liver and group B of right lobe of liver in rabbits respectively, and the color ultrasound was performed 1 week, 2 weeks, 3 weeks and 4 weeks after inoculation, respectively. Ct and routine pathological examination. 22 New Zealand White Rabbit models of VX2 Renal carcinoma They were randomly divided into two groups: group A (n = 11) and group B (n = 11), and group B (n = 11) received percutaneous 18G needle implantation of VX2 tumor tissue suspension under B-ultrasound guidance, respectively, and group B (n = 2) received color Doppler CT and routine pathological examination at 1 week, 2 weeks, 3 weeks and 4 weeks after inoculation. 22 New Zealand White Rabbit models of VX2 Breast Cancer Each group was randomly divided into two groups (11 rats in each group). The VX2 tumor tissue suspension was implanted with 18G needle in each group. Group B was treated with local skin VX2 tumor tissue transplantation, and inoculated under the second papilla pad on the left side of the chest wall of rabbit, and then inoculated under the second papilla pad on the left side of the chest wall. Two weeks and three weeks and four weeks were performed respectively by color Doppler imaging and conventional pathological examination. Establishment of New Zealand White Rabbit with brain tumor Model of C4X.VX2, 12 New Zealand White Rabbit, 12 New Zealand White Rabbits, 12 New Zealand White Rabbits, Randomly divided into two groups (each group, 6 rats, respectively, using minimally invasive localization of skull drilling tissue block transplantation method: group A: 1 or 2 tumor tissue blocks of about the size of 1mm3 were cut in advance to be implanted into the preformed skull foramen) and minimally invasive localization of skull drilling. Group B: VX2 tumor tissue suspension 0.3ml was injected into the skull foramen. Ct and Mr were performed 1 week, 2 weeks and 3 weeks and 4 weeks after inoculation respectively. The changes of nervous system signs and histopathological changes were observed after natural death. Statistics of success rate of tumor inoculation; observation of postoperative complications: observe the gross manifestations and metastasis of the tumor; observe the natural survival of animals; postmortem examination; tumor tissue fixed by 10% formalin, pathological examination. Result 1. The tumor foci were found in 22 rabbits with VX2 liver cancer model. The total tumorigenic rate was 72.710 / 22. The success rate of liver Zuo Ye inoculation group was 90.910 / 11a, and the success rate of liver right lobe inoculation group was 54.5g / 7 / 110.There was no significant difference between the two groups (P0.05A). 2. The tumor foci were found in 15 rabbits with VX2 renal cell carcinoma model. The total success rate of inoculation was 68.2 / 22%, the success rate of left kidney inoculation group was 54.5 / 11%, and the success rate of right kidney inoculation group was 81.8% / 11%. There was no significant difference between the two groups in the success rate of inoculation (P 0.05). 3. VX2 breast cancer model: 18 experimental rabbits found tumor foci, the total success rate of inoculation was 81.8% 22%, the success rate of group A was 90.9% 10%, and the success rate of group B was 72.7% 811%. There was no significant difference between the two groups in the success rate of inoculation (P 0.05). 4. The total success rate of VX2 brain tumor was 41.7% / 12%, the success rate was 83.3% / 6% in group A, and the success rate was 0% / 6% in group B) there was a significant difference between the two groups (P0.01%) in the VX2 brain tumor model (n = 5) and in group A (n = 5) with a total success rate of 41.7% / 12% (n = 5), and in group A (n = 5), the success rate was 83.3% (n = 5) and group B (n = 5). Conclusion Rabbit VX2 liver cancer model, rabbit VX2 renal cell carcinoma model, rabbit VX2 breast cancer model by tissue suspension injection, and rabbit VX2 brain tumor model were established by percutaneous puncture and implantation of tissue block under the guidance of color ultrasound. In order to make the process more simple and time-saving, the dynamic detection and evaluation of tumor foci by color Doppler CT and Mr plain scan can more accurately and timely observe the imaging changes such as the occurrence and development of tumor foci.
【学位授予单位】：泰山医学院
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R-332

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