血管内皮生长因子165基因促进人脂肪间充质干细胞的增殖

发布时间：2018-05-12 00:15

本文选题：组织工程 + 干细胞　；参考：《中国组织工程研究》2015年28期

【摘要】：背景:目前自体脂肪移植已广泛运用于美容整形和修复创伤导致的软组织缺损的修复,有研究表明,移植后1年移植脂肪存活率为20%-80%,因此,在移植后的早期及时、充分的血供建立,对于移植脂肪的存活是非常重要的。目的:观察血管内皮生长因子165转染人脂肪间充质干细胞的增殖情况。方法:体外传代培养人脂肪间充质干细胞,将重组血管内皮生长因子165基因入腺病毒液和空病毒液转染至脂肪间充质干细胞内,分别设为实验组和对照组,另设正常培养的细胞为空白组。结果与结论:RT-PCR,Western blot,MTT检测显示,与对照组和空白组相比,实验组血管内皮生长因子165 m RNA和蛋白的表达及细胞增殖均增高(P0.05)。结果证实,腺病毒承载的血管内皮生长因子165基因转染脂肪间充质干细胞后不仅可以持续的表达目的蛋白,同时也能显著促进脂肪间充质干细胞的增殖。
[Abstract]:Background: at present, autologous fat transplantation has been widely used in the repair of soft tissue defects caused by cosmetic surgery and wound repair. Studies have shown that the survival rate of fat grafts in one year after transplantation is 20 to 80, so it is timely in the early stage after transplantation. Adequate blood supply is essential for fat graft survival. Aim: to observe the proliferation of human adipose mesenchymal stem cells transfected with vascular endothelial growth factor 165. Methods: human adipose mesenchymal stem cells were cultured in vitro. The recombinant vascular endothelial growth factor 165 gene was transfected into adenovirus and empty virus into adipose mesenchymal stem cells respectively as experimental group and control group. The normal cultured cells were set as blank group. Results compared with the control group and the control group, the expression of vascular endothelial growth factor 165m RNA and protein and cell proliferation in the experimental group were higher than those in the control group (P 0.05). The results showed that the transfection of vascular endothelial growth factor 165 gene into adipose mesenchymal stem cells by adenovirus could not only continuously express the target protein, but also significantly promote the proliferation of adipose mesenchymal stem cells.
【作者单位】：辽宁医学院附属第一医院临床生物样本中心;辽宁医学院附属第一医院肿瘤科;辽宁医学院附属第一医院学科科研处;
【基金】：辽宁省科技厅科技计划项目(2013225305)~~
【分类号】：R329.2

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