促红细胞生成素对面神经损伤后运动神经元TNF-α及Bax表达的作用

发布时间：2018-05-14 02:12

本文选题：红细胞生成素(Erythropoietin + Epo)　；参考：《吉林大学》2010年硕士论文

【摘要】： 目的:探讨大剂量(5000U/kg体重)重组人促红细胞生成素(rhEpo)对面神经横断后面神经核内肿瘤坏死因子-α(TNF-α)及B细胞淋巴瘤基因-2相关性x蛋白(Bax)表达的作用,以及该作用与运动神经元存活率之间的关系。方法:本实验中共使用42只Wistar大鼠(体重250g-300g),不分性别。将其中40只大鼠随机分为2组:实验组(Epo组)和对照组(盐水组),每组20只。实验组给予5000U/kg体重的Epo,腹腔注射,连续给药5天;对照组同法给予等量生理盐水。给药第三天将两组共40只大鼠右侧面神经主干在出茎乳孔处横断。左侧不做处置。剩余2只大鼠不做任何处置作为空白对照。术后每周给药一次(对照组给予等量生理盐水)至处死。实验组和对照组大鼠分别于面神经损伤后第3天,第7天,第14天,第21天及第28天时,各处死4只,取脑组织包埋固定。将包含面神经核的脑干连续冠状切片,光镜下计数存活神经元的百分率,并应用免疫组化染色方法检测面神经核内TNF-α及Bax的表达。结果:在面神经横断后的14天, 21天和28天,Epo组的面神经运动神经元存活率明显较盐水组高。在Epo组和盐水组,面神经核内TNF-α的表达在损伤后3天至14天逐渐增加,14天后开始下降,损伤后前14天TNF-α(+)神经元百分率与面神经运动神经元存活率之间存在显著负线性相关性;Bax的表达在面神经损伤后3天至21天逐渐增加,28天时开始下降,损伤后前21天Bax(+)神经元百分率与面神经运动神经元存活率之间存在显著负线性相关性。面神经损伤后给予Epo可以显著抑制面神经核内TNF-α表达的增加,但Epo对Bax的表达无明显抑制作用(P0.05)。结论:在面神经损伤后,面神经核内TNF-α和Bax表达增加,大剂量的Epo可以抑制面神经核内TNF-α表达的增加,减轻炎症反应,提高了面神经运动神经元的存活率。Epo对Bax的表达无明显抑制作用。
[Abstract]:Objective: to investigate the effect of recombinant human erythropoietin (rhEpob) at a high dose of 5000U / kg body weight on the expression of tumor necrosis factor- 伪 (TNF- 伪) and Bax-associated gene -2 x in the posterior nucleus of facial nerve transection. And the relationship between this action and the survival rate of motor neurons. Methods: 42 Wistar rats (weight 250 g-300 g) were used without sex. Forty rats were randomly divided into two groups: experimental group (EPO group) and control group (saline group, 20 rats in each group). The experimental group was injected intraperitoneally with 5000U/kg body weight for 5 days, while the control group was given the same amount of normal saline. On the third day of administration, the right facial nerve trunk of 40 rats in both groups was transected at the caulomastoid foramen. The left side is not disposed of. The remaining 2 rats were not treated as blank control. Once a week after operation (the control group was given the same amount of normal saline) to death. On the 3rd, 7th, 14th, 21st and 28th days after facial nerve injury, 4 rats were killed in the experimental group and the control group respectively. The percentage of surviving neurons was counted by a continuous coronal section of the brain stem containing the facial nucleus, and the expression of TNF- 伪 and Bax in the nucleus of the facial nerve was detected by immunohistochemical staining. Results: the survival rate of facial motor neurons in the EPO group was significantly higher than that in the saline group at 14 days, 21 days and 28 days after the transection of the facial nerve. In Epo group and saline group, the expression of TNF- 伪 in the nucleus of facial nerve increased gradually from 3 to 14 days after injury, and began to decrease after 14 days of injury. There was a significant negative linear correlation between the percentage of TNF- 伪 () neurons and the survival rate of facial motoneurons on the 14th day after injury. There was a significant negative linear correlation between the percentage of Bax() neurons and the survival rate of facial motor neurons 21 days after injury. After facial nerve injury, Epo could significantly inhibit the expression of TNF- 伪 in the nucleus of facial nerve, but Epo had no obvious inhibitory effect on the expression of Bax. Conclusion: after facial nerve injury, the expression of TNF- 伪 and Bax in the nucleus of facial nerve is increased. Large dose of Epo can inhibit the expression of TNF- 伪 in the nucleus of facial nerve and alleviate the inflammatory reaction. The survival rate of facial motoneurons was increased. EPO had no inhibitory effect on the expression of Bax.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R363

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