建立乙醇、HBV双因素所致肝癌癌前病变小鼠模型的探讨

发布时间：2018-05-14 17:32

本文选题：乙醇 + HBV转基因小鼠　；参考：《中国中医基础医学杂志》2015年11期

【摘要】：目的:建立乙醇、HBV双因素诱导的肝癌癌前病变小鼠模型。方法:将32只HBV转基因小鼠,按随机数字表法分为16周对照组和模型组、22周对照组和模型组四组各8只,通过比较小鼠肝重和肝脏指数变化及肝脏病理变化来评价模型的建立情况。结果:模型组肝重和肝脏指数明显增加,肝脏形态学变化显示22周模型组肝损伤最严重,肝细胞层次1~3层增加,肝索排列紊乱,肝细胞不典型增生有小细胞性改变,细胞具有异型性。结论:乙醇、HBV双因素刺激可加重肝损伤,22周时模型组出现肝癌癌前病理变化,肝癌癌前病变动物模型的建立对于进一步探讨肝癌癌前病变的发病机制具有重要意义。
[Abstract]:Objective: to establish a mouse model of precancerous lesion of liver cancer induced by alcohol and HBV. Methods: 32 HBV transgenic mice were randomly divided into control group (n = 8), model group (n = 8) and model group (n = 8). The establishment of the model was evaluated by comparing the changes of liver weight, liver index and liver pathology in mice. Results: the liver weight and liver index in the model group were significantly increased. The morphological changes of the liver showed that the liver injury was the most serious in the 22-week model group, the level of hepatic cells was increased in layer 1 and 3, the arrangement of hepatic cord was disordered, and the atypical proliferation of hepatocytes had small cellular changes. Cells have heterogeneity. Conclusion: the double factor stimulation of HBV with ethanol can aggravate the liver injury in the model group. The establishment of animal model of precancerous lesion of liver cancer is of great significance for the further study of the pathogenesis of precancerous lesion of liver cancer.
【作者单位】：中国中医科学院博士后科研流动站;刘尚义国医大师传承工作室;贵阳中医学院;
【基金】：国家自然科学基金资助项目(81160417,81460697) 贵州省研究生工作站计划(黔教研合JYSZ字[2014]018) 贵州省科学技术厅-贵阳中医学院科学技术联合基金项目(黔科合LH字[2014]7336号)
【分类号】：R735.7;R-332

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