定抽颗粒对抽动障碍模型小鼠行为学及氨基酸类神经递质的影响

发布时间：2018-05-17 00:16

本文选题：抽动障碍 + 定抽颗粒　；参考：《江苏中医药》2015年07期

【摘要】：目的:探索定抽颗粒治疗儿童抽动障碍的作用机制。方法:72只ICR小鼠随机分为正常对照组、模型组、氟哌啶醇组和定抽颗粒高、中、低剂量组,每组12只。除正常对照组外,其余各组小鼠予亚氨基二丙腈(IDPN)腹腔注射诱导抽动障碍模型。造模当日起,正常对照组和模型组给予蒸馏水,其余组每日分别给予氟哌啶醇(1.38mg/kg)、定抽颗粒(5.76g/kg、2.88g/kg、1.44g/kg),连续灌胃3周。末次给药后1h进行刻板行为、运动行为、自主活动测定,翌日采用高效液相色谱法检测血浆及脑组织中γ-氨基丁酸(GABA)、天门冬酸(ASP)、谷氨酸(GLU)含量。结果:定抽颗粒各剂量组小鼠刻板行为、运动行为评分和自主活动次数均明显低于模型组(P0.01,P0.05)。模型组小鼠脑组织及血清中GABA含量明显低于正常对照组(P0.01),脑组织中GLU、ASP含量明显高于正常对照组(P0.01);定抽颗粒各剂量组脑组织、血清中GABA含量明显高于模型组(P0.01),脑组织中GLU、ASP含量明显低于模型组(P0.01)。结论:定抽颗粒可明显改善抽动障碍模型小鼠行为学改变,其可能的作用机制与抑制兴奋性氨基酸类神经介质、兴奋抑制性氨基酸类神经递质有关。
[Abstract]:Objective: to explore the mechanism of Dingqi granule in the treatment of children with tic disorder. Methods Seventy-two ICR mice were randomly divided into normal control group, model group, haloperidol group and Dingqi granule group with 12 rats in each group. In addition to the normal control group, the other mice were injected intraperitoneally with iminodipropionitrile (IDPN) to induce tic disorder model. From the same day, the normal control group and the model group were given distilled water, the other groups were given haloperidol 1.38 mg / kg per day, and Dingqi granule was 5.76 g / kg ~ 2.88 g 路kg ~ (-1) 路kg ~ (-1) ~ (-1). The contents of GABA, ASPU, GLU in plasma and brain tissue were determined by high performance liquid chromatography (HPLC) at 1 hour after the last administration. Results: the scores of stereotype, motor behavior and autonomous activity in Dingqi granule group were significantly lower than those in model group (P 0.01 and P 0.05). The content of GABA in brain tissue and serum of model group was significantly lower than that in normal control group (P 0.01), and the content of GLUASP in brain tissue was significantly higher than that in normal control group (P 0.01). The content of GABA in serum was significantly higher than that in model group (P 0.01), and the content of GLU in brain tissue was significantly lower than that in model group (P 0.01). Conclusion: Dingqi granule can obviously improve the behavioral changes in mice with tic disorder, and its possible mechanism is related to the inhibition of excitatory amino acid neurotransmitters and excitatory inhibitory amino acid neurotransmitters.
【作者单位】：南京中医药大学附属医院儿科;南京中医药大学;
【基金】：国家级重点学科南京中医药大学中医儿科学第二期开放研究课题(EZK2012019)
【分类号】：R285.5;R-332

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