3T3-L1前脂肪细胞分化过程中自噬的发生与线粒体数目的改变

发布时间：2018-05-26 13:14

本文选题：自噬 + 脂肪细胞分化　；参考：《南京医科大学学报(自然科学版)》2015年12期

【摘要】：目的 :探索3T3-L1前脂肪细胞分化过程中自噬的发生与线粒体数目改变的关系。方法 :培养3T3-L1前脂肪细胞,利用电子显微镜技术观察细胞分化过程中自噬小泡的形成与变化、线粒体数目的改变,同时运用Western blot检测NRF1、mt TFA的表达。结果:电镜结果显示:在3T3-L1前脂肪细胞分化过程中,自噬小泡出现在细胞分化的第2天,随后迅速增加,第4天自噬小泡的面积达到最大,第6天开始消失;线粒体数目在分化第2天显著下降,第4天开始逐渐增加,到第6天超过分化前的水平并在第8天恢复;在分化过程中的自噬小泡内,有被吞噬的线粒体。Western blot结果则显示调控线粒体合成的转录因子NRF1、mt TFA的表达在分化第2天显著下降,第4天迅速升高,至分化末期再次下降。结论 :3T3-L1前脂肪细胞分化过程的早中期形成了大量的自噬小泡,包括一些线粒体自噬,这些线粒体自噬的发生可能是分化早期线粒体数目减少的原因之一。
[Abstract]:Aim: to investigate the relationship between autophagy and mitochondrial number during preadipocyte differentiation in 3T3-L1. Methods: 3T3-L1 preadipocytes were cultured. The formation and change of autophagy vesicles and the number of mitochondria were observed by electron microscopy, and the expression of NRF1mt TFA was detected by Western blot. Results: the electron microscope showed that during the differentiation of adipocytes before 3T3-L1, autophagic vesicles appeared on the second day of differentiation, then increased rapidly, the area of autophagic vesicles reached the maximum on the 4th day, and disappeared on the 6th day. The number of mitochondria decreased significantly on the second day of differentiation, increased gradually on the 4th day, exceeded the pre-differentiation level on the 6th day and recovered on the 8th day. The results of phagocytic mitochondria. Western blot showed that the expression of NRF1mt TFA, a transcription factor regulating mitochondrial synthesis, decreased significantly on the second day of differentiation, increased rapidly on the 4th day, and decreased again at the end of differentiation. Conclusion A large number of autophagy vesicles, including some mitochondrial autophagy, were formed in the early and middle stages of the differentiation of the preadipocytes. The occurrence of these mitochondria autophagy may be one of the reasons for the decrease in the number of mitochondria in the early stage of differentiation.
【作者单位】：南京医科大学江苏省人类功能基因组学重点实验室;南京绿叶制药有限公司长效和靶向制剂国家重点实验室;
【基金】：国家自然科学基金资助(H0704)
【分类号】：R329.2

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