应激对大鼠生精功能及消化系统GnRH及其受体表达的影响

发布时间：2018-05-28 10:59

本文选题：持续光照 + 恐吓应激　；参考：《南京师范大学》2008年硕士论文

【摘要】： 应激(stress是指机体在受到各种内外环境因素刺激时所发生的非特异性全身反应。长期处于应激状态,机体的自稳态将改变,从而诱发机体产生疾病。连续的光照刺激对内分泌系统特别是垂体-性腺轴产生重要改变,导致个体体重和性腺发育出现异常,影响GnRH、LH分泌的强度和持久性。此外,长日照会导致大鼠胰腺和小肠的淀粉酶的活性,影响消化系统功能。某些军人在遭受战争环境刺激下会丧失应付能力,并伴有精神障碍,称之为作战应激反应(combatstress reaction CRS);发展到后期会导致创伤后应激性障碍(post-traumaticstress disorder,PTSD)。本实验模拟战争环境建立了两个应激模型:持续光照应激模型和战争恐惧应激模型。分别在两个应激模型下检测消化系统的GnRH及其受体的表达。并研究了在光照应激模型下,大鼠性腺的发育情况。光照应激组大鼠睾丸重量均比对照组偏高,但差异不明显(P＞0.05)。附睾重量与对照组相比差异不明显(P＞0.05)。光照应激组精子相对计数高于相应对照组,差异显著(P＜0.05)。光照应激组胃底腺壁细胞、胃小凹上皮细胞呈GnRH、GnRHR阳性。免疫反应阳性细胞定位与对照组无区别。光照应激组GnRH的平均灰度值高于相应对照组,持续光照2w时,GnRH的灰度值达到最高,光照2w到4w时,实验组与对照组相比差异均显著(P＜0.05)。胃的GnRHR表达量比相应对照组升高,持续光照1w后,实验组与相应的对照组相比差异显著(P＜0.05);持续光照2w时,GnRHR的表达达到最高水平,与对照组相比,差异极显著(P＜0.01);光照3w到4w后,GnRHR的表达水平有所降低,但与相应的对照组相比,差异仍显著(P＜0.05)。随着光照时间的延长,实验组大鼠GnRH和GnRHR mRNA均升高,光照应激2w后,实验组GnRH mRNA与对照组相比,具有显著性差异(P＜0.05)。在光照应激3w后,实验组GnRHR mRNA与对照组相比具有显著性差异(P＜0.05)。恐惧应激下,急性应激组、慢性应激组胃底腺壁细胞、胃小凹上皮细胞、肠黏膜层上皮细胞呈GnRHR阳性,和相应对照组相比,没有明显差异。阳性反应物质定位于胞膜和胞质中,胞核呈阴性。急性应激条件下,大鼠胃中GnRHR表达量与对照组相比下降不显著(P＜0.05)。慢性应激中,GnRHR的表达量与对照组相比,差异有统计学意义(P＜0.01)。而在大鼠的小肠中,实验组大鼠比对照组大鼠GnRHR的表达量增加了29.3%。在急性应激条件下,GnRHR表达有增多的趋势,但与对照组相比没有显著差异(P＞0.05);慢性应激组与相应的对照组相比,GnRHR表达明显增加(P＜0.05)。结论:持续光照对大鼠睾丸和附睾的重量无影响,但可影响精子的数量。光照应激模型和恐惧应激模型都能改变GnRH的分泌频率,影响GnRH的分泌表达水平。随着应激时间的延长,消化系统内GnRH及其受体的蛋白表达水平、mRNA水平也随之变化。实验结果表明消化道可以产生GnRH,GnRH通过其受体介导,以自分泌和旁分泌的机制对消化系统产生直接调节作用。
[Abstract]:Stress (stress refers to the non specific systemic reaction that occurs when the body is stimulated by a variety of internal and external environmental factors. For a long time in stress state, the body's self stability will change and induce the body to produce disease. Continuous illumination stimulates important changes in the endocrine system, especially the pituitary gonadal axis, leading to individual weight and gonadal glands. " Abnormal development affects the intensity and persistence of GnRH, LH secretion. In addition, long sunshine can lead to the activity of amylase in the pancreas and small intestine of the rat and affect the digestive system function. Some servicemen lose their ability to cope with war environment and have mental disorders, called combatstress reaction CRS; Post-traumaticstress disorder (PTSD) will develop later.
In this experiment, two stress models were established to simulate the war environment: the continuous light stress model and the war fear stress model. The expression of GnRH and its receptors in the digestive system were detected under two stress models, and the development of the gonadal development of rats under the light stress model was also studied.
The weight of the testis in the light stress group was higher than that in the control group, but the difference was not obvious (P > 0.05). The weight of epididymis was not significant compared with the control group (P > 0.05). The relative count of sperm in the light stress group was higher than that in the control group (P < 0.05).
Light illuminated group of gastric fundus parietal cells, gastric concave epithelial cells were GnRH, GnRHR positive. The location of immunoreactive cells was not different from that of the control group. The average gray value of GnRH in the light stress group was higher than that of the control group. The gray value of GnRH reached the highest when the light illumination was 2W, and the difference was significant between the experimental group and the control group when the light was 2W to 4W (P < 0.). 05) the expression of GnRHR in the stomach was higher than that in the corresponding control group. After 1W, the experimental group was significantly different from the corresponding control group (P < 0.05), and the expression of GnRHR reached the highest level when 2W was sustained. Compared with the control group, the difference was very significant (P < 0.01); the expression level of GnRHR decreased after the light 3W to 4W, but it was compared with the corresponding control group. The difference was still significant (P < 0.05). With the prolongation of light time, the GnRH and GnRHR mRNA in the experimental group increased, and the GnRH mRNA in the experimental group was significantly different from the control group (P < 0.05) after the photoperiod stimulated 2W (P < 0.05). The GnRHR mRNA in the experimental group was significantly different from that of the control group (P < 0.05).
In the acute stress group, the gastric fundus parietal cells, the gastric concave epithelial cells and the intestinal mucosa epithelial cells were GnRHR positive in the acute stress group and the chronic stress group. Compared with the corresponding control group, there was no significant difference. The positive reaction substance was located in the cytoplasm and cytoplasm, and the nucleus was negative. The expression of GnRHR in the stomach of the rats was compared with the control group. The decrease was not significant (P < 0.05). In the chronic stress, the expression of GnRHR was statistically significant (P < 0.01) compared with the control group (P < 0.01). In the small intestine of the rat, the expression of GnRHR in the rats of the experimental group increased the increasing tendency of 29.3%. in the acute stress condition, but there was no significant difference compared with the control group (P (0.05) compared with the corresponding control group, the expression of GnRHR increased significantly in chronic stress group (P < 0.05).
Conclusion: continuous illumination has no influence on the weight of the testis and epididymis, but affects the number of sperm. Both the light stress model and the fear stress model can change the secretion frequency of GnRH and affect the level of the expression of GnRH. As the time of stress prolongs, the protein expression level of GnRH and its receptor in the digestive system and the level of mRNA also change. The results indicate that GnRH can be produced in the alimentary tract, and GnRH mediates direct regulation of the digestive system through its receptor mediated autocrine and paracrine mechanisms.
【学位授予单位】：南京师范大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R363

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