气虚血瘀型宫环出血病证结合大鼠模型的建立及其特性研究

发布时间：2018-05-28 15:50

  本文选题：气虚血瘀 + 宫环出血病　； 参考：《湖南中医药大学》2010年硕士论文

【摘要】： 目的：为了加强对于气虚血瘀型宫环出血病证结合大鼠模型的研究,从更深层次和角度来研究气虚血瘀型宫环出血的特点和病因病机,为本病的治疗提供新的治疗方法和思路。 方法：将24只SD大鼠分为空白组、置环组、模型组、治疗组4组,每组各6只。对除空白组外3组置环后,模型组、治疗组采用复合式造模28天,建立气虚血瘀型宫环出血病证结合大鼠模型。从造模第1天起节食,强迫游泳,第14天起皮下注射肾上腺激素,治疗组第21天起采用宫环Ⅱ号方进行对抗治疗7天。观察4组大鼠的一般行为表现及体重变化,子宫组织形态涂片,分析蛋白质芯片各指标。 结果：模型组大鼠出现气虚、乏力、便溏等表现,体重较造模前明显减轻(P0.01),子宫组织形态涂片呈现内膜上皮细胞坏死脱落,炎症细胞浸润等。蛋白质芯片各指标中子宫组织中IFN-γ及IL-4相对表达水平模型组较空白组、置环组均明显降低(P0.01),治疗组较模型组升高(0.01P0.05)；MIP-3α相对表达水平模型组较空白组明显降低(P0.01),与置环组比较无统计学意义(P0.05),治疗组较模型组升高(0.01P0.05)；VEGF相对表达水平模型组与空白组、置环组比较无统计学意义(P0.05),治疗组较模型组降低(0.01P0.05)。余指标无统计学意义(P0.05)。 结论：对上环后大鼠节食、强迫游泳及皮下注射肾上腺素可导致大鼠一般行为表现及体重变化,子宫组织形态及子宫组织蛋白质芯片各指标发生改变,可证明建立气虚血瘀型宫环出血病证结合大鼠模型成功。
[Abstract]:Objective: in order to strengthen the study on the syndromes of uterine ring hemorrhage of Qi deficiency and blood stasis type combined with rat model, to study the characteristics and etiology and pathogenesis of uterine ring hemorrhage of Qi deficiency and blood stasis type from a deeper level and an angle, and to provide a new treatment method and thought for the treatment of this disease. Methods: 24 SD rats were divided into blank group, ring setting group, model group and treatment group with 6 rats in each group. The model group and treatment group were treated with compound model for 28 days to establish the model of uterine ring hemorrhage syndrome of Qi deficiency and blood stasis combined with rat model. From the first day of the model, forced swimming, subcutaneous injection of adrenal hormone on the 14th day, the treatment group was treated with Gong Huan 鈪，

本文编号：1947300