兔脊神经节与迷走神经下节形态学对比观察
本文选题:兔 + 脊神经节 ; 参考:《新疆医科大学》2008年硕士论文
【摘要】: 目的旨在对比观察脊神经节细胞形态之间的差异及脊神经节与迷走神经下神经节细胞之间的差异。方法取健康成年新西兰白兔16只,共取颈神经节124对,胸神经节182对,腰神经节112对,迷走神经下神经节16对。经常规HE染色观察神经元形态、胞体大小与细胞类型的关系;通过特殊染色观察尼氏体形状。利用ITEM图像分析系统测量神经元的大小。数据经SPSS11.0统计软件处理。结果1.脊神经节大型、中型和小型神经元各占脊神经节神经元百分比是8.3%、31.6%、60.1%;迷走神经下节大型、中型和小型神经元各占迷走神经下节神经元百分比是39.1%、56.3%、4.7%。脊神经节小神经元尼氏体染色多呈细颗粒状、胞浆染色较深;大、中型神经元的尼氏体染色呈颗粒状和块状、胞浆染色有浅有深;迷走神经下节尼氏体呈分散颗粒状,亦有神经元呈细小均匀的斑块。2.脊神经节颈部、胸部和腰部的大、中、小型神经元之间存在差异(p=0.00)。脊神经节与迷走神经下节神经元平均直径经秩和检验Z=-3.505,有统计学差异。3.在脊神经节颈1~4神经节、颈6~8和胸1神经节、胸2~胸12神经节、腰1~3神经节、腰4~7神经节神经元比较中,除颈6~8和胸1神经节与腰4~7神经节神经元之间比较无统计学差异外,其余两两之间比较有显著性差异(p㩳0.05)。结论1.脊神经节以中、小型神经元为主,迷走神经下节以大、中型神经元为主。2.脊神经节各部细胞大小之间比较存在差异(p㩳0.05)。
[Abstract]:Objective to compare the morphological differences of spinal ganglion cells and the differences between spinal ganglion cells and inferior vagal ganglion cells. Methods Sixteen healthy adult New Zealand rabbits were collected, including 124 pairs of cervical ganglion, 182 pairs of thoracic ganglion, 112 pairs of lumbar ganglion and 16 pairs of inferior vagus ganglion. The morphology of neurons, the relationship between cell body size and cell type were observed by routine HE staining, and the shape of Nissl body was observed by special staining. The size of neurons was measured by ITEM image analysis system. The data are processed by SPSS11.0 statistical software. Result 1. The percentage of large, medium and small neurons in the spinal ganglion was 8.3and 31.60.The percentage of large, medium and small neurons in the inferior vagus ganglion was 39.1and 56.3respectively. The Nissl bodies of small neurons of spinal ganglion were mostly fine granular, the cytoplasmic staining was deep, the staining of large and medium neurons was granular and massive, the staining of cytoplasm was shallow and deep, and the Nissl body of inferior ganglion of vagus was dispersed granular. There were small and uniform plaques of neurons. There is a difference between the large, middle and small neurons in the cervical, thoracic and lumbar regions of the spinal ganglion. The mean diameters of spinal ganglion and inferior vagus ganglion neurons were measured by rank sum test. There was no significant difference in the number of neurons in the spinal ganglion 1 ganglion, cervical 6 ganglion, thoracic 1 ganglion, thoracic 2 ~ thoracic 12 ganglion, lumbar 1 3 ganglion and lumbar 4 7 ganglion neurons, except that there was no significant difference between cervical 68 and thoracic 1 ganglion and lumbar 47 ganglion neurons. There was significant difference between the other two groups. Conclusion 1. The spinal ganglion mainly consists of small and medium neurons, while the inferior vagus ganglion is mainly composed of large and medium neurons. There was a significant difference in cell size between different parts of spinal ganglion.
【学位授予单位】:新疆医科大学
【学位级别】:硕士
【学位授予年份】:2008
【分类号】:R322
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