日本血吸虫核糖体蛋白SjRPS4基因及蛋白联合免疫保护性价值的研究

发布时间：2018-07-10 20:29

本文选题：日本血吸虫 + SjRPS4　；参考：《南昌大学》2010年硕士论文

【摘要】：目的：研究日本血吸虫核糖体蛋白(SjRPS4)基因和蛋白联合免疫对小鼠的保护效果。方法：将SjRPS4 DNA疫苗和重组蛋白疫苗间隔2周三次分别或联合免疫昆明鼠,2周后,每鼠感染尾蚴20条,45天后剖杀小鼠,计数成虫,肝、肠、粪及每雌子宫虫卵数。结果：联合免疫组与生理盐水组相比较,获得了49.7%的减虫率、62.6%的粪减卵率、46.0%的每雌子宫减卵率、49.0%的肝减卵率、54.3%的肠减卵率(P均0.01),均显著高于单独的SjRPS4 DNA疫苗组36.2%的减虫率、48.7%的粪减卵率、35.2%的每雌子宫减卵率、41.8%的肝减卵率、40.5%的肠减卵率(P均0.05),及单独蛋白免疫组38.2%的减虫率、50.1%的粪减卵率、38.9%的每雌子宫减卵率、42.4%的肠减卵率(P均0.05)。结论：SjRPS4 DNA疫苗及重组蛋白疫苗均可诱导小鼠产生一定的免疫保护作用,且联合免疫方案保护效果好于单一疫苗。
[Abstract]:Aim: to study the protective effect of SjRPS4 gene and protein combination immunization on mice. Methods: Kunming mice were immunized with SjRPS4 DNA vaccine and recombinant protein vaccine for 2 weeks. After each mouse was infected with 20 cercariae for 45 days, the number of adult worms, liver, intestine, feces and eggs per female uterus were counted. Results: the combined immunization group was compared with the saline group. 49.7% fecal egg reduction rate 46.0% liver egg reduction rate 54.3% (P 0.01) were significantly higher than that of SjRPS4 DNA vaccine group (36.2% fecal egg reduction rate 48.7% fecal egg reduction rate per female uterus). The oocyte reduction rate of liver was 41.8% and that of intestine was 40.5% (P 0.05), and that of single protein immunized group was 38.2% and 50.1% respectively. The rate of egg reduction per female uterus was 38.9% and 42.4% respectively (P 0.05). Conclusion both the DNA vaccine of SjRPS4 and the recombinant protein vaccine can induce immune protection in mice, and the protective effect of the combined immunization regimen is better than that of the single vaccine.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R392

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