甲醛遗传毒性与氧化损伤研究
发布时间：2018-07-15 16:06
【摘要】： 甲醛是一种常见的装修型化学性室内空气污染物,同时也是一种全球性的环境污染物。它具有污染来源广、时间长、水平高、毒性种类多等特点,因而已成为目前我国环境卫生领域研究的热点和难点。甲醛暴露对人体健康的影响主要涉及：呼吸道和眼部的刺激作用、神经行为改变、生殖毒性、致敏作用和免疫毒性、氧化损伤、遗传毒性和癌症等诸多方面。本文主要研究甲醛的氧化毒性和遗传毒性,以及说明其分子机制,这对全面和系统的了解甲醛毒性具有十分重要的意义,并为室内空气质量的改善提供更科学的依据。本研究以大鼠肝细胞为研究对象,通过检测8-羟基脱氧鸟苷含量来探讨其遗传毒性,检测丙二醛含量来研究其氧化毒性。研究结果如下： 1甲醛致遗传毒性作用 为了探讨外源性化合物暴露致内脏细胞DNA氧化损伤程度的定量检测方法,以8-羟基脱氧鸟苷为分子生物标志物,以甲醛为模式外源性化合物,应用大鼠肝细胞悬液进行体外染毒实验研究。实验设置甲醛染毒的终浓度分别为0、5、15、45μmol/L,在大鼠肝细胞悬液染毒1h后,检测肝细胞悬液中的8-羟基脱氧鸟苷(8-OHdG)研究结果显示,随着甲醛浓度升高,大鼠肝细胞中8-OHdG呈升高趋势(F=59.55,p0.01),高浓度组(45/μmol/L) 8-OHdG含量具有显著性(p〈0.01),中浓度组(15μmol/L)的这种差别也具有显著性(p0.01),低浓度组(5μmol/L)的这种差异没有显著性(p0.05)。因此,8-OHdG不但可以用于血液和尿液样品的检测,而且采用本项研究摸索出的前处理方法可以很好地定量测定内脏细胞DNA氧化损伤的程度。 2甲醛致氧化损伤效应 以大鼠肝脏细胞为研究材料,测定肝脏细胞中MDA的含量,检测甲醛对小鼠肝细胞的氧化损伤效应。结果显示：随着甲醛染毒浓度的上升,大鼠肝脏细胞MDA含量也随之上升(F=75.82,p〈0.01),并且与对照组相比,小鼠肝脏细胞MDA含量依次升高,而且这种升高在FA染毒浓度为15μmol/L时就出现显著性差异(P0.05)；高浓度组(45μmol/L) MDA含量与对照组的差异具极显著性(p0.01)。证明高浓度甲醛引起肝细胞膜脂质的严重损伤。
[Abstract]:Formaldehyde is a common decoration chemical indoor air pollutant, but also a global environmental pollutant. It has the characteristics of wide source of pollution, long time, high level and many kinds of toxicity, so it has become a hot and difficult point in the field of environmental health in our country. The effects of formaldehyde exposure on human health include respiratory tract and eye irritation, neurobehavioral changes, reproductive toxicity, sensitization and immune toxicity, oxidative damage, genetic toxicity and cancer. This paper mainly studies the oxidative and genetic toxicity of formaldehyde and explains its molecular mechanism, which is of great significance for a comprehensive and systematic understanding of formaldehyde toxicity, and provides a more scientific basis for the improvement of indoor air quality. In this study, the genetic toxicity of 8-hydroxydeoxyguanosine and the oxidative toxicity of 8-hydroxydeoxyguanosine were studied by detecting the content of malondialdehyde (MDA) in rat hepatocytes. The results are as follows: 1 the genotoxic effect of formaldehyde in order to study the quantitative detection method of DNA oxidative damage in visceral cells induced by exogenous compounds, Using 8-hydroxydeoxyguanosine as molecular biomarker and formaldehyde as model exogenous compound, rat hepatocyte suspension was used to study the toxicity in vitro. The final concentration of formaldehyde was set at 45 渭 mol 路L ~ (-1), respectively. The 8-hydroxydeoxyguanosine (8-OHdG) in rat hepatocyte suspension was detected with the increase of formaldehyde concentration for 1 hour after exposure to formaldehyde, and the results showed that the concentration of 8-hydroxydeoxyguanosine (8-OHdG) in rat hepatocyte suspension increased with the increase of formaldehyde concentration. The level of 8-OHdG in rat hepatocytes was increased (FV 59.55, p0.01), the content of 8-OHdG in high concentration group (45 / 渭 mol / L) was significant (p < 0.01), the difference in middle concentration group (15 渭 mol / L) was also significant (p0.01), there was no significant difference in low concentration group (5 渭 mol / L) (p0.05). Therefore, 8-OHdG can not only be used for the detection of blood and urine samples, Moreover, the pre-treatment method developed in this study can be used to determine quantitatively the degree of DNA oxidative damage in visceral cells. 2 oxidative damage induced by formaldehyde in rat liver The visceral cells were used as the research materials. The content of MDA in liver cells and the oxidative damage effect of formaldehyde on mouse hepatocytes were determined. The results showed that the content of MDA in rat liver cells increased with the increase of formaldehyde concentration (FF75.82, p < 0.01), and the content of MDA in liver cells of mice increased in turn compared with the control group. Moreover, there was a significant difference in the level of MDA between the high concentration group (45 渭 mol / L) and the control group (p0.01) when the FA concentration was 15 渭 mol / L (P0.05), and the concentration of MDA in the high concentration group (45 渭 mol / L) was significantly higher than that in the control group (P0.01). It was proved that high concentration of formaldehyde caused serious damage to lipids of liver cell membrane.
【学位授予单位】：华中师范大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R363
本文编号：2124629
[Abstract]:Formaldehyde is a common decoration chemical indoor air pollutant, but also a global environmental pollutant. It has the characteristics of wide source of pollution, long time, high level and many kinds of toxicity, so it has become a hot and difficult point in the field of environmental health in our country. The effects of formaldehyde exposure on human health include respiratory tract and eye irritation, neurobehavioral changes, reproductive toxicity, sensitization and immune toxicity, oxidative damage, genetic toxicity and cancer. This paper mainly studies the oxidative and genetic toxicity of formaldehyde and explains its molecular mechanism, which is of great significance for a comprehensive and systematic understanding of formaldehyde toxicity, and provides a more scientific basis for the improvement of indoor air quality. In this study, the genetic toxicity of 8-hydroxydeoxyguanosine and the oxidative toxicity of 8-hydroxydeoxyguanosine were studied by detecting the content of malondialdehyde (MDA) in rat hepatocytes. The results are as follows: 1 the genotoxic effect of formaldehyde in order to study the quantitative detection method of DNA oxidative damage in visceral cells induced by exogenous compounds, Using 8-hydroxydeoxyguanosine as molecular biomarker and formaldehyde as model exogenous compound, rat hepatocyte suspension was used to study the toxicity in vitro. The final concentration of formaldehyde was set at 45 渭 mol 路L ~ (-1), respectively. The 8-hydroxydeoxyguanosine (8-OHdG) in rat hepatocyte suspension was detected with the increase of formaldehyde concentration for 1 hour after exposure to formaldehyde, and the results showed that the concentration of 8-hydroxydeoxyguanosine (8-OHdG) in rat hepatocyte suspension increased with the increase of formaldehyde concentration. The level of 8-OHdG in rat hepatocytes was increased (FV 59.55, p0.01), the content of 8-OHdG in high concentration group (45 / 渭 mol / L) was significant (p < 0.01), the difference in middle concentration group (15 渭 mol / L) was also significant (p0.01), there was no significant difference in low concentration group (5 渭 mol / L) (p0.05). Therefore, 8-OHdG can not only be used for the detection of blood and urine samples, Moreover, the pre-treatment method developed in this study can be used to determine quantitatively the degree of DNA oxidative damage in visceral cells. 2 oxidative damage induced by formaldehyde in rat liver The visceral cells were used as the research materials. The content of MDA in liver cells and the oxidative damage effect of formaldehyde on mouse hepatocytes were determined. The results showed that the content of MDA in rat liver cells increased with the increase of formaldehyde concentration (FF75.82, p < 0.01), and the content of MDA in liver cells of mice increased in turn compared with the control group. Moreover, there was a significant difference in the level of MDA between the high concentration group (45 渭 mol / L) and the control group (p0.01) when the FA concentration was 15 渭 mol / L (P0.05), and the concentration of MDA in the high concentration group (45 渭 mol / L) was significantly higher than that in the control group (P0.01). It was proved that high concentration of formaldehyde caused serious damage to lipids of liver cell membrane.
【学位授予单位】：华中师范大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R363
【引证文献】
相关期刊论文 前2条
1 李亚琳;;液态甲醛对雄性小鼠生殖毒性的研究[J];动物医学进展;2012年12期
2 李亚琳;;甲醛对小鼠肝脏毒性作用的研究[J];动物医学进展;2013年04期
，本文编号：2124629
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