豚鼠与SD大鼠高脂血症模型的比较研究

发布时间：2018-07-21 12:08

【摘要】： 目的:通过比较豚鼠、SD大鼠两种动物高脂血症模型的特点及两种动物高脂血清对HUVEC损伤的影响,为治疗和预防高脂血症的研究选择理想的动物模型提供试验依据。方法:将豚鼠、SD大鼠各20只分别随机分为对照组和模型组。对照组用基础饲料饲养,模型组用高脂饲料饲养。各组均于连续饲养30d后采血,测定各项血脂指标。取肝脏、主动脉进行病理学检查。收集各组血清,并以此血清分组培养人脐静脉血管内皮细胞(HUVEC),用酶联免疫法(ELISA)检测培养上清液的血管性假血友病因子(vWF)。结果:30d造模期后,豚鼠高脂血症模型组与SD大鼠高脂血症模型组相比:1、豚鼠的体重增长变化更为显著(P＜0.01)。2、豚鼠TC、TG、LDL-C升高程度明显高于SD大鼠(P＜0.01)。3、豚鼠肝脏脂肪变程度较SD大鼠重。4、豚鼠转氨酶(ALT、AST)明显升高(P＜0.01),而SD大鼠无变化。5、豚鼠肝组织SOD活性降低较SD大鼠明显(P＜0.05)。6、豚鼠的动脉硬化指数(AI)升高明显,其主动脉内膜能形成动脉粥样硬化。7、豚鼠高脂血清对HUEVC的损伤程度更重;豚鼠上清液vWF含量升高明显,且48h较24h升高更为显著(P＜0.01)。结论:豚鼠较具有抗AS形成能力的大鼠而言,更适用于高脂血症、AS等的研究。
[Abstract]:Objective: to compare the characteristics of two animal hyperlipidemia models in guinea pig Sprague-Dawley (SD) rats and the effects of two kinds of animal hyperlipidemic serum on HUVEC injury, so as to provide experimental basis for selecting ideal animal models for the treatment and prevention of hyperlipidemia. Methods: 20 guinea pig SD rats were randomly divided into control group and model group. The control group was fed with basic diet, while the model group was fed with high fat diet. Blood samples were collected after 30 days of continuous feeding, and blood lipid indexes were measured. The liver and aorta were taken for pathological examination. Human umbilical vein endothelial cells (HUVEC) were cultured in each group. The levels of von Willebrand factor (vWF) in the supernatant were detected by Elisa. Results after 30 days of modeling, Compared with SD rat hyperlipidemia model group, the weight gain of guinea pigs in the guinea pig hyperlipidemia model group was more significant (P < 0.01) .2.The increase of LDL-C in guinea pigs was significantly higher than that in SD rats (P < 0.01). 3. The fatty degree of guinea pig liver was heavier than that of SD rats. (4) the activity of SOD in guinea pig liver tissue was significantly lower than that in SD rat (P < 0.05), and the arteriosclerosis index (AI) of guinea pig was significantly higher than that of SD rat (P < 0.01), while the activity of SOD in guinea pig liver was significantly lower than that in SD rat (P < 0.01), while the activity of SOD in guinea pig liver was lower than that in SD rat (P < 0.05). The intima of aorta could form atherosclerosis. 7, the damage degree of guinea-pig hyperlipidemia serum to HUEVC was more serious, and the content of vWF in guinea-pig supernatant was significantly higher than that in 24h (P < 0.01). Conclusion: the guinea pig is more suitable for the study of hyperlipidemia and as than the rats with anti-atherogenic ability.
【学位授予单位】：昆明医学院
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：R589.2;R-332

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