兔股动脉三氯化铁血栓模型的方法学研究

发布时间：2018-07-23 16:11

【摘要】： 目的:探索应用三氯化铁溶液外敷联合夹闭动脉建立兔股动脉血栓模型的方法。方法:选取新西兰大白兔21只,将其双侧股动脉随机分为:联合法夹闭10分钟组(A组)、联合法夹闭7分钟组(B组)、联合法夹闭5分钟组(C组)、联合法夹闭3分钟组(D组)、外敷对照组(E组)、夹闭对照组(F组),每组7条股动脉。所有联合法组在10%三氯化铁溶液外敷的基础上分别给予夹闭股动脉10、7、5、3分钟;外敷对照组(E组):给予10%三氯化铁溶液股动脉外敷;夹闭对照组(F组):夹闭股动脉10分钟。用多普勒血流仪连续监测股动脉的血流量,同时留取股动脉的二维及彩色多普勒超声图像,1小时为实验终点,实验结束对实验段股动脉进行病理分析。兔股动脉血栓模型成功建立的标准为闭塞性血栓形成。结果:联合法夹闭10分钟组(A组)、联合法夹闭7分钟组(B组)、联合法夹闭5分钟组(C组)、联合法夹闭3分钟组(D组)建模成功率均分别为100%、100%、71%、29%,所形成的血栓均为富含血小板的混合型血栓;外敷对照组(E组)和夹闭对照组(F组),1小时内均未形成闭塞性血栓。结论:三氯化铁溶液外敷联合夹闭动脉建立兔股动脉血栓模型的方法,所需三氯化铁溶液浓度较低,血栓形成率高,是一种较实用的血栓模型制作方法。
[Abstract]:Aim: to explore the method of establishing rabbit femoral artery thrombosis model by external application of ferric chloride solution combined with clipping artery. Methods: 21 New Zealand white rabbits were selected. The bilateral femoral arteries were randomly divided into three groups: combined occlusion for 10 minutes (group A), combined occlusion for 7 minutes (group B), combined occlusion for 5 minutes (group C), combined occlusion for 3 minutes (group D) and external application for control group (group E). In control group (group F), there were 7 femoral arteries in each group. On the basis of external application of 10% ferric chloride solution, all the combined treatment groups were given 107U 5min clamping femoral artery for 3 minutes respectively, group E: 10% ferric chloride solution for external application of femoral artery, and group F clamping control group (group F): clamping femoral artery for 10 minutes. The blood flow of femoral artery was continuously monitored by Doppler flow meter, and 2-D and color Doppler ultrasound images of femoral artery were taken for 1 hour as the end point of the experiment. The pathological analysis of the femoral artery was carried out at the end of the experiment. The standard of successful establishment of rabbit femoral artery thrombosis model is occlusion thrombosis. Results: the success rates of modeling in group A (group A), group B (group B), group C (group C), group C (group C) and group D (group D) were 100% and 100%, respectively. Mixed thrombus containing platelets; No occlusive thrombus was found in the control group (E group) and clamped control group (F group) within 1 hour. Conclusion: ferric chloride solution is a practical method for the establishment of rabbit femoral artery thrombosis model by external application of ferric chloride solution combined with occlusion of artery. The concentration of ferric trichloride solution is lower and the rate of thrombosis is high.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R-332

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