鸟结核分枝杆菌刺激巨噬细胞后对细胞骨架蛋白β-actin的调控机制研究

发布时间：2018-07-24 10:38

【摘要】：目的研究鸟结核分枝杆菌刺激巨噬细胞后其对巨噬细胞骨架蛋白及其调节蛋白的作用机制。方法 RT-PCR方法分析M.avium刺激巨噬细胞后cofilin-1,β-actin基因的表达水平,同时Western blot方法从蛋白水平分析M.avium刺激巨噬细胞后β-actin、cofilin-1蛋白的表达水平。流式细胞术检测M.avium刺激巨噬细胞后巨噬细胞凋亡及坏死的情况。结果β-actin基因及其蛋白在巨噬细胞受M.avium刺激后表达显著下调,而β-actin蛋白的调节蛋白cofilin-1表达显著增强。同时,M.avium刺激巨噬细胞后诱导巨噬细胞凋亡显著增多。结论 M.avium刺激巨噬细胞后可通过调控β-actin及cofilin-1蛋白的表达,从而诱导巨噬细胞凋亡或坏死。
[Abstract]:Objective to study the effect mechanism of Mycobacterium tuberculosis on macrophage cytoskeleton and its regulatory protein after stimulating macrophages. Method RT-PCR method was used to analyze the expression level of cofilin-1, beta -actin gene after M.avium stimulation of macrophages, and Western blot method to analyze beta -actin, cofili after M.avium stimulation of macrophage from protein level. The expression level of n-1 protein. Flow cytometry was used to detect the apoptosis and necrosis of macrophage after M.avium stimulated macrophage. Results the expression of beta -actin gene and its protein decreased significantly after M.avium stimulation, while the expression of the regulatory protein of beta -actin protein was significantly enhanced. Meanwhile, M.avium stimulated macrophage to induce the expression. The apoptosis of macrophages was significantly increased. Conclusion M.avium stimulated macrophage by regulating the expression of beta -actin and cofilin-1 protein, thus inducing macrophage apoptosis or necrosis.
【作者单位】：江苏大学附属昆山医院;中南大学肿瘤研究所;重庆市肿瘤研究所病理科;
【基金】：昆山市科技局立项项目(KS1425)~~
【分类号】：R378.911

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