丹蒌片对兔动脉粥样硬化模型ERS相关基因表达的影响

发布时间：2018-07-26 18:39

【摘要】：目的:建立兔动脉粥样硬化模型,研究丹蒌片对兔粥样硬化病变及内质网应激(ERS)相关基因免疫球蛋白重链结合蛋白(BIP)表达的影响,为丹蒌片临床的应用提供实验依据。方法:雄性日本大耳白兔24只随机分为正常组、模型组、可定组和可定+丹蒌片联合用药组,每组6只。正常组饲喂普通饲料,模型组在普通饲料中加入2%胆固醇及0.02%蛋氨酸;可定组、可定+丹蒌片组在普通饲料中加入2%胆固醇及0.02%蛋氨酸及相应的对照药物,复制家兔动脉粥样硬化病变模型。分别在用药前与饲养9周后检测胆固醇(TC),甘油三酯(TG),低密度脂蛋白胆固醇(LDL-C),高密度脂蛋白胆固醇(HDL-C)值,载脂蛋白A(Apo A),载脂蛋白B(Apo B);饲养9周后取实验动物胸主动脉上段,苏木素-伊红(HE)常规染色后观察组织形态变化;应用原位杂交技术检测BIP基因表达。结果:与正常组比较,模型组兔血脂TC,TG,LDL-C,Apo B含量明显升高,HDL-C,Apo A含量明显降低(P0.05);与模型组比较,可定组、丹蒌片联合用药组明显降低兔血脂TC,TG,LDL-C,Apo B含量,明显升高HDL-C,Apo A含量(P0.01)。HE染色正常组血管内膜形态正常;模型组内膜严重增生,内皮细胞损伤;可定组血管内膜轻度增生;可定+丹蒌片组血管内膜形态接近正常。原位杂交结果显示,模型组BIP蛋白的表达明显升高;可定组及可定+丹蒌片组可下调BIP蛋白的表达。结论:丹蒌片联合可定能延缓动脉粥样硬化的发展,且效果显著。
[Abstract]:Aim: to establish rabbit atherosclerosis model and to study the effect of Danlou tablet on the expression of immunoglobulin heavy chain binding protein (BIP) in rabbit atherosclerotic lesion and endoplasmic reticulum stress (ER) stress, and to provide experimental evidence for clinical application of Danlou tablet. Methods: a total of 24 male Japanese white rabbits were randomly divided into normal group, model group, codicodine group and combination therapy group with 6 rabbits in each group. The model group was fed with 2% cholesterol and 0.02% methionine in the normal diet, and the control group was treated with 2% cholesterol and 0.02% methionine in the normal diet, and 2% cholesterol and 0.02% methionine in the control group. Rabbit atherosclerosis model was established. Cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and high density lipoprotein cholesterol (HDL-C) were measured before and after 9 weeks of feeding respectively. Apolipoprotein A (Apo A), apolipoprotein B (Apo B); was fed for 9 weeks. The histomorphology of hematoxylin-eosin (HE) was observed and the expression of BIP gene was detected by in situ hybridization. Results: compared with the normal group, the content of serum lipids in the model group increased significantly (P 0.05), the content of HDL-CU Apo A in the model group was significantly decreased (P0.05), compared with the model group, the content of serum lipids, TGG, LDL-CU and Apo B was significantly decreased in the combination treatment group, and compared with the model group, the serum lipids in the model group were significantly lower than those in the control group. The content of HDL-CnApo A was significantly increased (P0.01). The morphology of vascular intima was normal in normal group, severe hyperplasia of intima and injury of endothelial cell in model group, mild hyperplasia of intima in Coding group, and close to normal in group A. The results of in situ hybridization showed that the expression of BIP protein was significantly increased in the model group, and the expression of BIP protein was down-regulated in the Coding group and the control group. Conclusion: Danglou tablet combined with can delay the development of atherosclerosis, and the effect is remarkable.
【作者单位】：河南中医学院基础医学院;苏州大学医学部;
【基金】：河南省基础与前沿技术研究计划项目(112300410156) 郑州市科技创新团队项目(121PCXTD520) 河南省高等学校青年骨干教师计划项目(2013GGJS-093)
【分类号】：R285.5;R-332

【参考文献】