京尼平对激素非依赖性前列腺癌裸鼠模型的抑瘤作用研究

发布时间：2018-07-29 10:54

【摘要】：目的建立激素非依赖性前列腺癌裸鼠模型,评价京尼平的抗肿瘤作用,并探讨其可能的治疗机制。方法采用颈背部皮下种植肿瘤细胞的方法建立激素非依赖性前列腺癌裸鼠模型,随机分为京尼平低、中、高剂量组及对照组;分别给予20 mg/kg、40 mg/kg、80mg/kg京尼平灌胃及生理盐水灌胃;测量裸鼠体质量、肿瘤体积并计算抑瘤率,流式细胞术检测细胞的增殖和凋亡情况。聚合酶链反应检测解偶联蛋白2(UCP2)的基因表达情况。结果各组裸鼠肿瘤体积在干预前比较差异均无统计学意义(P均0.05);京尼平能显著抑制肿瘤生长,并随着给药浓度的增高,肿瘤生长抑制率、增殖抑制作用及凋亡率逐渐增高;京尼平能抑制肿瘤细胞中UCP2的表达,中、高剂量组UCP2的基因及蛋白的表达显著低于低剂量组及对照组(P均0.05)。结论京尼平对激素非依赖性前列腺癌裸鼠模型有明显的抑瘤作用,其作用机制可能与抑制UCP2的表达有关。
[Abstract]:Objective to establish hormone independent prostate cancer model in nude mice to evaluate the anti-tumor effect of geniapine and to explore its possible therapeutic mechanism. Methods the nude mice model of hormone independent prostate cancer was established by subcutaneous implantation of tumor cells in the neck and back. The nude mice were randomly divided into three groups: geniapine low, middle and high dose groups and control group, and were given 20 mg / kg 40 mg / kg of genistein and 80 mg / kg of normal saline respectively. The body mass, tumor volume and tumor inhibition rate of nude mice were measured, and cell proliferation and apoptosis were detected by flow cytometry. The gene expression of uncoupling protein 2 (UCP2) was detected by polymerase chain reaction. Results there was no significant difference in tumor volume between the groups before intervention (P 0.05), geniapine significantly inhibited tumor growth, and with the increase of drug concentration, tumor growth inhibition rate, proliferation inhibition and apoptosis rate gradually increased. Geniapine inhibited the expression of UCP2 in tumor cells. The expression of UCP2 gene and protein in medium and high dose groups was significantly lower than that in low dose group and control group (P 0. 05). Conclusion geniapine has obvious inhibitory effect on steroid-independent prostate cancer in nude mice, and its mechanism may be related to the inhibition of UCP2 expression.
【作者单位】：贵阳医学院附属医院;河北省保定市第三医院/保定市肿瘤医院;江苏省扬州市江都人民医院;
【基金】：贵阳市科技计划项目(筑科合同[2011103]26号)
【分类号】：R737.25;R-332

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