运动性心房颤动兔模型的建立

发布时间：2018-08-02 19:17

【摘要】：目的建立由运动诱发的心房颤动(房颤)动物模型并评价其效果。方法 24只成年健康新西兰大耳白兔,分为对照组(n=8)、中强度组(n=8)、高强度组(n=8)。对照组不进行任何训练,中强度组和高强度组采用兔实验跑台不同强度运动,每天1 h或一次性力竭(不足1 h),每周5 d,持续12周(第一周为适应性训练)。于运动前、运动后8周、12周通过超声心动图检测各组兔心房大小;运动结束后,24只兔采用离体心脏Langendorff系统进行灌流,行心房早搏程序刺激(S1S2)诱发房颤,记录房颤的诱发率。结果与对照组比较,中强度组和高强度组左、右房前后径在运动训练8周后均增加,12周后也增加,差异有统计学意义(P均0.05)。与中强度组比较,高强度组左、右房前后径在运动训练8周后均增加,12周后也增加,差异有统计学意义(P均0.05)。与对照组比较,中强度组(45%vs.60%)和高强度组(45%vs.90%)房颤发生率增加,差异有显著统计学意义(P均0.01)。结论长期高强度的跑台运动训练可使兔心房内径增加,房颤的诱发率增加。
[Abstract]:Objective to establish an animal model of exercise induced atrial fibrillation (AF) and evaluate its effect. Methods 24 healthy New Zealand white rabbits were divided into three groups: control group (n = 8), moderate strength group (n = 8) and high intensity group (n = 8). The control group did not perform any training, and the moderate and high intensity groups were used to exercise at different intensity in the experimental platform of rabbits, one hour per day or one time exhausted (less than 1 h), per week for 5 days, lasting for 12 weeks (the first week was adaptive training). Atrial size was measured by echocardiography before exercise, 8 weeks and 12 weeks after exercise, 24 rabbits were perfused with isolated heart Langendorff system after exercise, atrial fibrillation was induced by atrial premature beat program stimulation (S1S2), and the induced rate of atrial fibrillation was recorded. Results compared with the control group, the anterior and posterior diameter of left and right atrium in the middle intensity group and high intensity group increased after 8 weeks of exercise training and then increased after 12 weeks of exercise training. The difference was statistically significant (P 0.05). The anterior and posterior diameter of left and right atrium in high intensity group increased after 8 weeks of exercise training and then increased after 12 weeks of exercise training. The difference was statistically significant (P 0.05). Compared with the control group, the incidence of atrial fibrillation in the moderate intensity group (45 vs 60%) and the high intensity group (45 vs 90%) was significantly higher than that in the control group (P 0.01). Conclusion long-term high-intensity treadmill training can increase atrial diameter and induce atrial fibrillation in rabbits.
【作者单位】：山西医科大学第二临床医学院;北京军区总医院心血管疾病研究所;北京军区总医院超声科;北京大学第一医院心内科;
【分类号】：R541.75;R-332

【参考文献】