深圳市新分离两株基孔肯雅热病毒的分子遗传学分析

发布时间：2018-08-05 19:54

【摘要】：目的对2010~2012年深圳市报告的两起输入性基孔肯雅热病原体进行分子遗传学分析。方法利用C6/36细胞从病人血清中分离基孔肯雅病毒(Chikungunya virus,CHIKV),对分离得到的CHIKV进行全基因组测序和构建系统发生树,结合流行病学资料对其分子遗传特征进行分析。结果成功分离得到两株CHIKV,分别命名为SZ_20101028和SZ_20120702,SZ_20101028全基因组长为12377bp,SZ_20120823全基因组长为11893bp;构建系统发生树分析,结果表明,SZ_20101028是印度洋亚型,ECSA型的后裔。SZ_20120702为Asian亚型。结论 SZ_20101028与最近10年来在印度洋岛屿爆发流行新亚型且与2010年由输入性引发起东莞疫情爆发的毒株亲源性最高,为99%;而SZ_20120702为Asian亚型,不是近年来爆发流行株。
[Abstract]:Objective to analyze the molecular genetics of two imported Kikungunya fever pathogens reported in Shenzhen from 2010 to 2012. Methods Chikungunya virus was isolated from patients' serum by C6 / 36 cells. The whole genome of CHIKV was sequenced and phylogenetic tree was constructed. The molecular genetic characteristics were analyzed with epidemiological data. Results two strains of CHIKVwere successfully isolated and named as SZ_20101028 and SZS _ (20120702) respectively. The length of the whole genome of SZ _ (201028) was 12377bpmpmp, and the whole genome length of SZ _ (20823) was 11893bp.The result of phylogenetic tree analysis showed that SZZ20101028 was a descendant of Indian Ocean subtype (SZ2020702) and was subtype of Asian (S / Z20120702), and the result of phylogenetic tree analysis showed that SZZ20101028 was a subtype of Indian Ocean subtype. Conclusion the new subtype of SZ_20101028 epidemic in the Indian Ocean islands in the last 10 years and that of Dongguan outbreak caused by import in 2010 are the highest (99%), while SZ_20120702 is a Asian subtype, and is not a prevalent strain in recent years.
【作者单位】：深圳市龙华新区疾病预防控制中心;深圳市疾病预防控制中心;
【基金】：深圳市龙华新区科技计划资助项目(NO.20140916A1030029)
【分类号】：R373

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