MF59佐剂对肠道病毒71型灭活疫苗诱导小鼠体液免疫应答的影响

发布时间：2018-08-06 13:22

【摘要】：目的探讨MF59佐剂对肠道病毒71型(enterovirus 71,EV71)灭活疫苗诱导小鼠体液免疫应答的影响。方法将ICR小鼠随机分成10组:MF59佐剂+不同含量EV71灭活抗原(50 EU、100 EU)组、Al(OH)3佐剂0.5 mg+不同含量EV71灭活抗原(50 EU、100 EU)组和PBS阴性对照组,每组10只,分别经小鼠后肢肌肉和腹腔注射,200μl/只,共免疫2次,间隔4周(0、28 d)。分别于初免和加强免疫后28 d,采集小鼠尾静脉血,分离血清,采用微量中和试验法检测小鼠血清抗体水平。结果除阴性对照组外,各组小鼠血清抗体阳转率及中和抗体GMT值均随免疫剂量和免疫时间的延长呈升高趋势。初免后28 d,抗原含量为50 EU(低剂量)和100 EU(高剂量)各组中和抗体GMT均较低,组间差异均无统计学意义(P0.05)。加强免疫后28 d,低剂量各组小鼠血清中和抗体GMT与初免比较均略有升高(P0.05);高剂量各组小鼠血清中和抗体GMT与初免比较均明显升高,其中MF59佐剂肌肉注射组血清中和抗体GMT值最高(274.40),显著高于铝佐剂肌肉注射组(P0.05)。结论 MF59佐剂可显著增强EV71灭活疫苗诱导小鼠的体液免疫应答。
[Abstract]:Objective to investigate the effect of MF59 adjuvant on humoral immune response induced by inactivated vaccine of enterovirus 71 (enterovirus 71) in mice. Methods ICR mice were randomly divided into 10 groups (10 mice in each group) treated with different contents of EV71 inactivated antigen (50 EU100EU) and 50 (OH) 3 adjuvant 0.5 mg EV71 inactivated antigen (50 EUL 100EU) and PBS negative control group (10 mice). Immunization 2 times, at intervals of 4 weeks (0: 28 d).) Blood samples were collected from caudal vein of mice at 28 days after immunization, and the serum levels of antibody were detected by microneutralization test. Results except the negative control group, the serum antibody positive conversion rate and neutralizing antibody GMT value of each group showed an increasing trend with the prolongation of the immune dose and immune time. At 28 days after immunization, the GMT levels of neutralizing antibodies in 50 EU (low dose) and 100EU (high dose) groups were lower, and there was no significant difference between the two groups (P0.05). On the 28th day after immunization, the serum neutralization antibody (GMT) of low dose groups was slightly higher than that of the initial immunity (P0.05), and the serum neutralizing antibody GMT of the high dose groups was significantly higher than that of the initial immunity. The serum neutralization antibody GMT value of MF59 adjuvant injection group was the highest (274.40), which was significantly higher than that of aluminum adjuvant intramuscular injection group (P0.05). Conclusion MF59 adjuvant can significantly enhance the humoral immune response induced by EV71 inactivated vaccine in mice.
【作者单位】：中国医学科学院北京协和医学院医学生物学研究所病毒疫苗研究组云南省重大传染病疫苗研发重点实验室;诺华(中国)生物医学研究有限公司;
【分类号】：R392

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