葡聚糖硫酸钠在构建肝纤维化动物模型中的价值探讨
发布时间：2018-08-10 17:48
【摘要】：目的探讨葡聚糖硫酸钠(DSS)对BALB/C小鼠肝损伤及其纤维化的影响。方法 50只BALB/C小鼠随机分成正常组、对照组和观察组(6、12周),每组10只,对照组小鼠用5%四氯化碳(CCl4)橄榄油溶液诱导肝纤维化模型,观察组小鼠用3.5%DSS灌胃诱导肝损伤及纤维化模型。HE染色观察肝脏病理损伤,Massion染色观察肝脏纤维化情况,改良赖氏法检测血清谷丙转氨酶(ALT)和谷草转氨酶(AST)活性,ELISA法检测血清Ⅰ型前胶原羧基端原肽(PⅠCP)和Ⅲ型前胶原肽(PⅢNP)含量,免疫组化检测肝脏α-平滑肌肌动蛋白(α-SMA)表达变化。结果对照组和观察组小鼠各时间段肝脏的病理损伤明显,胶原纤维表达量增多,血清中ALT和AST活性增强、PⅠNP和PⅢNP含量增加,肝内α-SMA表达量增加,上述变化以对照组最明显,观察组12周次之,观察组与对照组比较,差异有统计学意义(P0.05)。结论 DSS能有效诱导BALB/C小鼠肝纤维化,其诱导机制值得深入研究。
[Abstract]:Objective to investigate the effects of sodium dextran sulfate (DSS) on liver injury and fibrosis in BALB/C mice. Methods Fifty BALB/C mice were randomly divided into control group (n = 10), control group (n = 10) and observation group (n = 10). The control group was treated with 5% CCl4 olive oil solution to induce hepatic fibrosis. The liver injury and fibrosis model induced by 3.5%DSS were used in the observation group. The pathological injury of liver was observed by HE staining. The activities of serum alanine aminotransferase (ALT) and glutamic oxalacetic transaminase (AST) were detected by modified Lai's method. The levels of type 鈪，
本文编号：2175739
[Abstract]:Objective to investigate the effects of sodium dextran sulfate (DSS) on liver injury and fibrosis in BALB/C mice. Methods Fifty BALB/C mice were randomly divided into control group (n = 10), control group (n = 10) and observation group (n = 10). The control group was treated with 5% CCl4 olive oil solution to induce hepatic fibrosis. The liver injury and fibrosis model induced by 3.5%DSS were used in the observation group. The pathological injury of liver was observed by HE staining. The activities of serum alanine aminotransferase (ALT) and glutamic oxalacetic transaminase (AST) were detected by modified Lai's method. The levels of type 鈪，
本文编号：2175739
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