转化生长因子α促进人内皮祖细胞增殖、迁移和黏附

发布时间：2018-08-11 08:50

【摘要】：目的:探讨转化生长因子α(transforming growth factorα,TGF-α)对人内皮祖细胞(endothelial progenitor cells,EPCs)单克隆形成、增殖、迁移和黏附细胞功能的影响及机制。方法:分离培养人内皮祖细胞,分别用浓度为1、5、10μg/L TGF-α诱导处理细胞,并设置PBS对照组和表皮生长因子受体酪氨酸激酶抑制剂EGFR-TKI组(同时加入10μg/L TGF-α和1∶1 000的EGFR-TKI)。利用单克隆形成实验、MTT法和Ed U法、Transwell法和细胞黏附实验检测不同浓度TGF-α对各组EPCs单克隆形成能力、细胞增殖、细胞迁移能力及黏附功能的影响;并通过Western blotting法检测不同浓度TGF-α对各组EPCs中表皮生长因子受体(epithelial growth factor receptor,EGFR)和血管内皮生长因子(vascular endothelial growth factor,VEGF)表达的影响。结果:不同浓度TGF-α均能显著诱导EPCs单克隆形成能力、增殖、迁移和黏附细胞功能,差异有统计学意义(P0.05),并可以被EGFR-TKI抑制。Western blotting检测发现TGF-α显著诱导EPCs中EGFR和VEGF的表达(P0.05),并呈浓度依赖性。结论:TGF-α能够显著促进人EPCs细胞单克隆形成、增殖、迁移和黏附相关细胞功能,并通过与EGFR结合诱导VEGF表达来发挥作用。
[Abstract]:Aim: to investigate the effect and mechanism of transforming growth factor 伪 (TGF- 伪) on the formation, proliferation, migration and adhesion of human endothelial progenitor cells (endothelial progenitor cells). Methods: human endothelial progenitor cells were isolated and cultured. The cells were treated with 10 渭 g / L TGF- 伪 at a concentration of 1 渭 g / L and treated with PBS control group and epidermal growth factor receptor tyrosine kinase inhibitor EGFR-TKI group (10 渭 g / L TGF- 伪 and 1:1 EGFR-TKI). The effects of different concentrations of TGF- 伪 on EPCs monoclonal formation ability, cell proliferation, cell migration and adhesion were detected by MTT assay, Ed U method and cell adhesion assay. The effects of different concentrations of TGF- 伪 on the expression of epidermal growth factor receptor (epithelial growth factor receptor EGFR) and vascular endothelial growth factor (VEGF) in EPCs were detected by Western blotting assay. Results: different concentrations of TGF- 伪 could significantly induce the ability of EPCs monoclonal formation, proliferation, migration and adhesion cell function. The difference was statistically significant (P0.05), and the expression of EGFR and VEGF in EPCs was significantly induced by TGF- 伪 by EGFR-TKI inhibition. Western blotting assay (P0.05), and it was found that TGF- 伪 could significantly induce the expression of EGFR and VEGF in a concentration-dependent manner. Conclusion: TGF- 伪 can significantly promote the formation, proliferation, migration and adhesion of human EPCs cells, and induce the expression of VEGF by binding to EGFR.
【作者单位】：三峡大学仁和医院心血管内科;三峡大学仁和医院ICU科;
【基金】：教育厅自然科学研究计划重点项目(No.D20091308)
【分类号】：R363.2

【相似文献】