大鼠原位肝移植模型建立及预输注5-Fu处理的同种异基因淋巴细胞诱导免疫耐受的初步观察

发布时间：2018-08-12 14:55

【摘要】：目的建立改良的大鼠原位肝移植模型和急性排斥反应模型;初步观察预输注5-Fu处理的同种异基因淋巴细胞对大鼠肝移植免疫耐受的影响,为临床诱导移植免疫耐受提供新的思路和方法。方法采用改良的“二袖套法”建立Wistar大鼠→SD大鼠原位肝移植急性排斥反应模型。实验分4组,均行Wistar至SD的大鼠肝移植,A组,对照组,仅行Wistar至SD的大鼠肝移植;B组,术前第7天与第4天各输注Wistar大鼠淋巴细胞(5×106/ml)悬液1ml;C组,术前第7天与第4天各输注5-Fu 7.5ug诱导过的Wistar大鼠淋巴细胞(5×106/ml)悬液1ml;D组,术前第7天与第4天各输注5-Fu 15ug诱导过的Wistar大鼠淋巴细胞(5×106/ml)悬液1ml。术后第7天观察移植肝的病理改变和各组受体的存活时间。结果成功建立改良的大鼠原位肝移植急性排斥反应模型。C组大鼠肝脏病理改变呈急性轻度排斥反应,肝细胞条索状排列基本整齐,肝小叶结构存在;中央静脉血管少量炎性细胞侵润;汇管区少量淋巴细胞侵润。A组呈急性重度排斥反应;B、D组呈急性中度排斥反应。C组优于A、B、D组。肝移植后受体大鼠生存时间:A组为6.7±1.5天,B组为10.3±2.7天,C组为19.0±5.3天,D组为11.7±3.9天。B,C,D组和A组差异显著(P0.01);C组和B,D组差异显著(P0.01);B,D组差异不显著(P0.05)。结论改良的“二袖套法”大鼠原位肝移植方法,手术时间短,成功率高,建立了稳定的大鼠原位肝移植模型,可满足本实验的要求。预输注低浓度5-Fu处理的同种异基因淋巴细胞可以诱导大鼠肝移植免疫耐受。
[Abstract]:Objective to establish a modified orthotopic liver transplantation model and acute rejection model, and to observe the effect of allogeneic lymphocytes pretreated with 5-Fu on the immune tolerance of liver transplantation in rats. To provide new ideas and methods for clinical induction of transplantation tolerance. Methods the model of acute rejection of orthotopic liver transplantation (OLT) in Wistar rats was established by modified "two cuff method". The experiment was divided into 4 groups, all of them were given Wistar to SD rat liver transplantation group A, control group, Wistar to SD rat liver transplantation group B, Wistar rat lymphocyte (5 脳 106/ml) suspension 1 ml C group 7 and 4 days before operation. On the 7th and 4th day before operation, the Wistar rat lymphocytes (5 脳 106/ml) suspensions induced by 5-Fu 7.5ug were infused into the Wistar rats at the 7th day and the 4th day before operation, and the Wistar lymphocytes (5 脳 106/ml) suspensions induced by 5-Fu 15ug were infused respectively on the 7th day and the 4th day before the operation in the group D, and 1 ml of the Wistar lymphocytes (5 脳 106/ml) were injected respectively on the 7th and 4th day before the operation. The pathological changes and survival time of the recipients were observed on the 7th day after operation. Results A modified rat model of acute rejection of orthotopic liver transplantation was established successfully. The pathological changes of the liver in group C were mild and acute, the hepatic cells were arranged neatly and the hepatic lobule structure existed. A small amount of inflammatory cells were infiltrated into the central venous vessels and a small amount of lymphocytes infiltrated in the catchment area. Group A showed acute and severe rejection. Group C was superior to group A in acute and moderate rejection. After liver transplantation, the survival time of recipient rats was 6.7 卤1.5 days in group B, 10.3 卤2.7 days in group C, 19.0 卤5.3 days in group C, 11.7 卤3.9 days in group D and 11.7 卤3.9 days in group D, and there was significant difference between group A and group A (P0.01). There was no significant difference between group C and group B (P0.01) (P0.05). Conclusion the modified "two-cuff method" for orthotopic liver transplantation in rats has the advantages of short operation time and high success rate. A stable orthotopic liver transplantation model has been established, which can meet the requirements of this experiment. Allogeneic lymphocytes treated with low concentration 5-Fu could induce liver transplantation immune tolerance in rats.
【学位授予单位】：广州医学院
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R657.3;R-332

【相似文献】