大鼠胎脑放射状胶质细胞表达乙酰胆碱转移酶

发布时间：2018-08-14 09:23

【摘要】：证实端脑背侧脑室区放射状胶质细胞是否表达ChAT(乙酰胆碱转移酶)以了解其是否产生胆碱能神经元。本实验采用免疫组织化学技术和电镜技术观察了大鼠端脑背侧产生的皮质锥体神经元的放射状胶质细胞形态、细胞分裂以及ChAT(乙酰胆碱转移酶)的表达。实验首先采用Vimentin(波形蛋白)对端脑培养细胞进行免疫组化染色。其结果表明为阳性表达。通过E14大鼠胚胎端脑细胞进行体外原代培养观察、培养细胞进行波形蛋白和乙酰胆碱转移酶双重荧光免疫细胞化学染色以在体外实验中确定放射状胶质细胞是否表达乙酰胆碱转移酶;并通过重叠同一视野内的荧光单染的图片来精确的体现放射状胶质细胞表达乙酰胆碱转移酶的位置。结果显示E14大鼠胚胎端脑细胞经血清培养聚集成团的细胞胞质内有呈Vimentin/ChAT免疫化学双染阳性表达。细胞聚集成团,细胞团内部双染阳性细胞较多,细胞团间可见伸出的彼此联系的突起,细胞沿突起在细胞团之间迁移,突起中也有Vimentin/ChAT免疫化学双染阳性表达。此结果在体外实验方面证实了E14大鼠端脑内的放射状胶质细胞表达乙酰胆碱转移酶。表明放射状胶质细胞具有神经干细胞性质,它不断地进行不对称分裂,产生胆碱能表型的成神经细胞,即胆碱能前体细胞。放射状胶质细胞在神经元发生期进行不对称分裂的同时已经决定了它所产生神经细胞的表型,合成神经递质乙酰胆碱。结果提示:(1)放射状胶质细胞可以产生胆碱能神经元;(2)放射状胶质细胞可能依据其产生不同神经元的类型分为不同亚群;(3)放射状胶质细胞可能在大脑皮层的发育过程中起重要作用。大鼠端脑放射状胶质细胞可产生皮质胆碱能神经元
[Abstract]:To confirm the expression of ChAT (acetylcholine transferase) in radiative glial cells of the dorsal ventricular region of the endencephalon to understand whether it produces cholinergic neurons. The morphology, cell division and expression of ChAT (acetylcholine transferase) of cortical pyramidal neurons produced at the dorsal end of the rat brain were observed by immunohistochemistry and electron microscopy. Firstly, Vimentin (vimentin) was used for immunohistochemical staining of cultured cells. The results showed positive expression. The primary culture of E14 rat embryonic brain cells was carried out in vitro. The cultured cells were stained with vimentin and acetylcholine transferase double fluorescent immunocytochemistry to determine whether the radial glial cells expressed acetylcholine transferase in vitro. The position of acetylcholinyltransferase (acetylcholinyltransferase) expressed by radial glial cells was precisely reflected by overlapping fluorescence monochromatic images in the same field. The results showed that Vimentin/ChAT immunocytochemical double staining was present in the cytoplasm of E14 rat embryonic brain cells. There were many double staining positive cells in the cell clusters, and the interlinked processes could be seen between the cell clusters. The cells migrated along the processes between the cell clusters, and there was Vimentin/ChAT immunocytochemical double staining positive expression in the processes. The results confirmed the expression of acetylcholine transferase in radiative glial cells of E14 rats in vitro. The results indicate that the radial glial cells have the nature of neural stem cells, and they divide unsymmetrically and produce cholinergic phenotypic neural cells, that is, cholinergic progenitor cells. The asymmetric division of radial glial cells during neuronogenesis has determined the phenotype of neurons and synthesized neurotransmitter acetylcholine. The results suggest that: (1) radiative glial cells can produce cholinergic neurons; (2) radial glial cells may be classified into different subgroups according to the types of neurons produced; (3) radial glial cells may have developed in the cerebral cortex. Cheng Zhong plays an important role. Cortical cholinergic neurons produced by radiative glial cells in rat telencephalon
【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R329

【相似文献】