大鼠放射性肾损伤模型制作及影像学评价
发布时间：2018-08-19 18:14
【摘要】：[摘要]目的：研究大鼠放射性肾损伤影像学模型制作方法,研究早期放射性肾损伤病理改变,动态观察大鼠单侧放射性肾损伤CT、MRI表现、彩色多普勒B超影像学表现及研究肾脏血流动力学改变,并评估放射性肾病的严重程度。 方法：80只SD大鼠随机分为对照组4组,照射组4组,每组10只,左肾单次分割20Gy电子线照射。照射后第4、12、24、36周分别行CT、MRI检测,观察影像学改变,照射后第4、12、24、36周用彩色多普勒超声检查,观察影像学改变并检测各时期肾血管多普勒参数的动态变化,同时观察病理改变。 结果：CT、MRI均显示大鼠放射性肾损伤,CT扫描显示第4周、第12周对照组与照射组左肾体积差异无统计学意义(P0.05),第24周、第36周对照组与照射组左肾体积值可见差异具有统计学意义(P0.05),其照射组左肾体积低于对照组体积值。照射组不同时间组体积值差异具有统计学意义(P0.05),随时间延长体积缩小。第4周对照组与照射组左肾肾实质厚度值差异无统计学意义(P0.05),第12周、第24周及第36周对照组与照射组左肾肾实质厚度值可见差异具有统计学意义(P0.05),其照射组左肾肾实质厚度低于对照组。照射组不同时间左肾肾实质厚度值差异具有统计学意义(P0.05),随时间延长肾实质厚度减少。MRI扫描显示第4周、第12周及第24周对照组与照射组大鼠左肾体积差异无统计学意义(P0.05),第36周对照组与照射组左肾体积值差异具有统计学意义(P0.05),其照射组左肾体积低于对照组体积值。照射组不同时间体积值差异具有统计学意义(F为3.821,P值0.05),随时间延长体积缩小。第4周、第12周对照组与照射组左肾肾实质厚度值差异无统计学意义(P0.05),第24周及第36周对照组与照射组左肾肾实质厚度值差异具有统计学意义(P0.05),其照射组左肾肾实质厚度低于对照组。照射组不同时间左肾肾实质厚度值差异具有统计学意义(P值0.05),随时间延长肾实质厚度减少。MRI可显示皮髓质分界不清。彩色多普勒B超显示肾实质回声逐渐增强,照射后第24周及第36周,肾脏体积及肾实质厚度与对照组比较肾脏体积缩小,实质变薄,差异有统计学意义(P0.05)。肾血管血流阻力指数(RI)逐渐升高,照射后36周,肾动脉RI值与对照组比较增高明显,差异有统计学意义(P0.05),照射后第24周及第36周,肾内动脉RI值与对照组比较明显增高,差异有统计学意义(P0.05)。病理检查显示4周后出现肾小球及肾小管损伤,并随时间逐渐加重。 结论： SD大鼠单侧肾单分割20Gy可以制作放射性肾损伤模型,肾脏发生明显病理变化。CT、MRI均可作为诊断放射性肾损伤的方法。彩色多普勒超声可以作为放射性肾损伤的临床检查手段,其血流动力学改变可反映放射性肾病病变严重程度。
[Abstract]:Objective: to study the imaging model of radiation-induced renal injury in rats, to study the pathological changes of early radiation-induced renal injury, and to dynamically observe the CT MRI findings of unilateral radiation-induced renal injury in rats. Color Doppler ultrasonography (CDFB) was used to study renal hemodynamic changes and to evaluate the severity of radiation nephropathy. Methods 80 Sprague-Dawley rats were randomly divided into control group (n = 4) and irradiation group (n = 10). The imaging changes were observed by CT MRI at 36-week after irradiation. The imaging changes were observed by color Doppler ultrasound at 36-week after irradiation. The changes of renal Doppler parameters were observed and the pathological changes were observed at the same time. Results CT scan showed that there was no significant difference in left renal volume between the control group and the irradiation group at the 12th week (P0.05). At the 36th week, there was significant difference in left renal volume between the control group and the irradiation group (P0.05), and the left renal volume in the irradiation group was lower than that in the control group. The volume value of different time groups in irradiation group was significantly different (P0.05), and the volume decreased with time. There was no significant difference in left renal parenchyma thickness between the control group and the irradiation group at the 4th week (P0.05). The left renal parenchyma thickness in the control group and the irradiation group was significantly lower than that in the control group at the 24th and 36th week (P0.05). The thickness of left renal parenchyma in the irradiation group was significantly different at different time (P0.05). The thickness of renal parenchyma decreased with time. MRI scan showed that the thickness of renal parenchyma decreased in the 4th week. At week 12 and week 24, there was no significant difference in left renal volume between the control group and the irradiation group (P0.05), but there was significant difference in the left renal volume between the control group and the irradiation group at the 36th week (P0.05), and the left renal volume in the irradiation group was lower than that in the control group. The volume value of irradiation group was significantly different at different time (F = 3.821, P = 0.05), and the volume decreased with the prolongation of time. Week 4, There was no significant difference in left renal parenchyma thickness between the control group and the irradiation group at the 12th week (P0.05), but there was significant difference in the left renal parenchyma thickness between the control group and the irradiation group at the 24th week and the 36th week (P0.05), and the thickness of the left kidney parenchyma in the irradiation group was lower than that in the control group. There was significant difference in left renal parenchyma thickness between different time groups (P 0.05). The thickness of renal parenchyma decreased with time. MRI could show that the boundary of dermatomedullary was not clear. Color Doppler ultrasound showed that the echo of renal parenchyma was gradually enhanced. At the 24th and 36th week after irradiation, the renal volume and the thickness of renal parenchyma were smaller and thinner than those of the control group (P0.05). Renal vascular resistance index (RI) increased gradually, and the RI value of renal artery increased significantly at 36 weeks after irradiation (P0.05), and the RI value of renal artery was significantly higher than that of control group at the 24th and 36th week after irradiation. The difference was statistically significant (P0.05). Pathological examination showed that glomerular and tubular injury occurred 4 weeks later and gradually aggravated with time. Conclusion: the model of radiation-induced renal injury can be made in SD rats with unilateral renal monolithral 20Gy, and the pathological changes of the kidney can be used as a diagnostic method for radiation-induced renal injury. Color Doppler ultrasound can be used as a clinical examination method for radiation-induced renal injury, and its hemodynamic changes can reflect the severity of radiation-induced nephropathy.
【学位授予单位】：天津医科大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R73-3;R-332
本文编号：2192436
[Abstract]:Objective: to study the imaging model of radiation-induced renal injury in rats, to study the pathological changes of early radiation-induced renal injury, and to dynamically observe the CT MRI findings of unilateral radiation-induced renal injury in rats. Color Doppler ultrasonography (CDFB) was used to study renal hemodynamic changes and to evaluate the severity of radiation nephropathy. Methods 80 Sprague-Dawley rats were randomly divided into control group (n = 4) and irradiation group (n = 10). The imaging changes were observed by CT MRI at 36-week after irradiation. The imaging changes were observed by color Doppler ultrasound at 36-week after irradiation. The changes of renal Doppler parameters were observed and the pathological changes were observed at the same time. Results CT scan showed that there was no significant difference in left renal volume between the control group and the irradiation group at the 12th week (P0.05). At the 36th week, there was significant difference in left renal volume between the control group and the irradiation group (P0.05), and the left renal volume in the irradiation group was lower than that in the control group. The volume value of different time groups in irradiation group was significantly different (P0.05), and the volume decreased with time. There was no significant difference in left renal parenchyma thickness between the control group and the irradiation group at the 4th week (P0.05). The left renal parenchyma thickness in the control group and the irradiation group was significantly lower than that in the control group at the 24th and 36th week (P0.05). The thickness of left renal parenchyma in the irradiation group was significantly different at different time (P0.05). The thickness of renal parenchyma decreased with time. MRI scan showed that the thickness of renal parenchyma decreased in the 4th week. At week 12 and week 24, there was no significant difference in left renal volume between the control group and the irradiation group (P0.05), but there was significant difference in the left renal volume between the control group and the irradiation group at the 36th week (P0.05), and the left renal volume in the irradiation group was lower than that in the control group. The volume value of irradiation group was significantly different at different time (F = 3.821, P = 0.05), and the volume decreased with the prolongation of time. Week 4, There was no significant difference in left renal parenchyma thickness between the control group and the irradiation group at the 12th week (P0.05), but there was significant difference in the left renal parenchyma thickness between the control group and the irradiation group at the 24th week and the 36th week (P0.05), and the thickness of the left kidney parenchyma in the irradiation group was lower than that in the control group. There was significant difference in left renal parenchyma thickness between different time groups (P 0.05). The thickness of renal parenchyma decreased with time. MRI could show that the boundary of dermatomedullary was not clear. Color Doppler ultrasound showed that the echo of renal parenchyma was gradually enhanced. At the 24th and 36th week after irradiation, the renal volume and the thickness of renal parenchyma were smaller and thinner than those of the control group (P0.05). Renal vascular resistance index (RI) increased gradually, and the RI value of renal artery increased significantly at 36 weeks after irradiation (P0.05), and the RI value of renal artery was significantly higher than that of control group at the 24th and 36th week after irradiation. The difference was statistically significant (P0.05). Pathological examination showed that glomerular and tubular injury occurred 4 weeks later and gradually aggravated with time. Conclusion: the model of radiation-induced renal injury can be made in SD rats with unilateral renal monolithral 20Gy, and the pathological changes of the kidney can be used as a diagnostic method for radiation-induced renal injury. Color Doppler ultrasound can be used as a clinical examination method for radiation-induced renal injury, and its hemodynamic changes can reflect the severity of radiation-induced nephropathy.
【学位授予单位】：天津医科大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R73-3;R-332
【参考文献】
相关期刊论文 前1条
1 陈建魁,尹秀云,牟兆钦,刘军,王桂林,秦茂权,杨永清,刘琳;急性放射病肾功能监测指标的探讨[J];中华放射医学与防护杂志;1997年01期
，本文编号：2192436
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