妊娠期母鼠接触SEB对新生鼠胸腺及外周T细胞的影响
发布时间：2018-08-23 12:30
【摘要】：目的： 金黄色葡萄球菌肠毒素B（Staphylococcal enterotoxin B，SEB）既是一种毒素性物质，又是葡萄球菌肠毒素家族9个血清型中最重要和研究较多的超抗原。SEB作为一种超抗原，无需经过抗原递呈细胞的识别和加工，就可以直接通过桥联抗原递呈细胞的MHCⅡ类分子结合槽外侧区与T淋巴细胞TCR的Vβ区，刺激机体免疫系统大量的T细胞活化。通常SEB进入机体后，初期可短暂激活Vβ8~+T细胞并诱发大量增殖，继而过度增殖的细胞发生凋亡导致克隆清除，而出现中枢或外周耐受。尽管有许多关于SEB对成年动物及新生鼠免疫器官、T淋巴细胞及细胞因子影响的研究，但妊娠期母鼠接触SEB对子代新生鼠细胞免疫的影响，目前国内外未见报道。因此，我们选用妊娠16天孕鼠尾静脉注射15μg SEB，同时建立PBS对照，在子代新生鼠出生后第0-5天检测胸腺、脾脏及外周血中CD4/CD8T细胞及TCR Vβ8~+T细胞亚群；通过新生鼠胸腺体外培养观察ConA或SEB刺激后胸腺细胞的分化发育；通过脾脏淋巴细胞体外培养观察其对ConA或SEB刺激的增殖应答情况。 方法： 1.选用清洁级成年SD大鼠，交配及确定受孕后，孕鼠饲养至妊娠16天随机分组，每组12只，实验组（SEB组）孕鼠通过尾静脉注射15μg的SEB，同时设立对照组，给予等体积的PBS（磷酸盐缓冲液）。 2.在妊娠期满（21天）时，用4%水合氯醛麻醉孕鼠后打开腹腔和子宫，取出胎鼠并分离胸腺和脾脏，称量胎体、胎盘、胸腺及脾脏的干湿重。 3.在新生鼠出生后的第0-5天，获取每组新生鼠的胸腺、脾脏和外周血，用网搓法制备胸腺及脾脏的单细胞悬液，脾脏的单细胞悬液及外周血用红细胞裂解液去除红细胞。将获得的细胞悬液分为两份，一份用荧光抗体CD3-FITC、CD4-APC和CD8-PE进行细胞染色，另一份用Vβ8.2-FITC、CD4-APC和CD8-PE进行染色，染色后经流式细胞仪检测T细胞亚群的比例。 4.无菌分离出生后第0天的两组新生鼠胸腺，采用胸腺器官培养法（thymus organculture，TOC）进行体外培养，并加入ConA或SEB共培养三天。在培养结束时收集胸腺并制备单细胞悬液，染色后经流式细胞仪检测胸腺中T细胞亚群的比例。 5.无菌分离出生后第3天的两组新生鼠脾脏，用淋巴细胞分离液分离脾脏淋巴细胞，体外与ConA或SEB连续共培养三天。在第1、2、3天培养结束时，收集脾脏淋巴细胞，染色后经流式细胞仪检测脾脏中T细胞亚群的比例。若检测脾脏淋巴细胞增殖能力时，在每一时间点结束前5小时加入3H-TDR共培养至结束，然后通过γ计数仪检测rpm. 结果： 1.妊娠期母鼠接触SEB后对胎鼠体重等指标的影响 妊娠期第16天母鼠静脉注射SEB后，可明显减少胎鼠胸腺及脾脏的湿重和干重(P0.01)；但与PBS组比较，SEB组的胎体及胎盘的干、湿重无统计学差异。2.妊娠期母鼠接触SEB对新生鼠中枢及外周CD4/CD8T细胞亚群的影响 2.1妊娠期母鼠接触SEB对新生鼠胸腺中CD4/CD8T细胞亚群的影响 妊娠期第16天母鼠接触SEB后，在新生鼠出生后的第0-5天，SEB组新生鼠胸腺中CD4~+T细胞的比例明显高于PBS组(P0.01或P0.05)，而SEB组CD8~+T细胞的比例明显低于PBS组的(P0.01或P0.05)。 2.2妊娠期母鼠接触SEB对新生鼠脾脏中CD4/CD8T细胞亚群的影响 在新生鼠出生后第0-5天，SEB组新生鼠脾脏中CD4~+T细胞的比例在各时间点均明显高于PBS组(P0.01或P0.05)。但两组在各时间点的CD8~+T细胞比例无统计学差异。 2.3妊娠期母鼠接触SEB对新生鼠外周血中CD4/CD8T细胞亚群的影响 在新生鼠出生后第0-5天，PBS组和SEB组新生鼠外周血CD4~+T细胞与CD8~+T细胞亚群比例的总体变化趋势与脾脏中的相类似。两组外周血中CD4/CD8T细胞比值均呈上升趋势，从第0-1天的比值小于1逐渐增加到之后的大于1，但SEB组在各时间点的CD4/CD8T细胞的比值均明显高于PBS组(P0.01或P0.05)。 3.妊娠期母鼠接触SEB对新生鼠中枢及外周TCR Vβ8.2~+T细胞的影响 妊娠期第16天母鼠接触SEB后，在新生鼠出生后的第0-3天，SEB组新生鼠胸腺中的CD4~+TCR Vβ8.2~+T细胞比例较PBS组的明显减少(P0.01或P0.05)，在第4-5天两组的CD4~+TCR Vβ8.2~+T细胞比例趋于平稳，且无统计学差异；而在出生后的第0-5天，SEB组新生鼠胸腺及外周血中CD8~+TCR Vβ8.2~+T细胞比例及外周血中的CD4~+TCR Vβ8.2~+T细胞比例均较PBS组的明显减少(P0.01或P0.05)。 4.妊娠期母鼠接触SEB对体外培养新生鼠胸腺分化发育的影响 4.1妊娠期母鼠接触SEB对体外培养新生鼠胸腺中CD4/CD8T细胞亚群的影响 PBS组和SEB组新生鼠胸腺体外与ConA或SEB共培养三天后，SEB组新生鼠胸腺中CD4~+T细胞及CD8~+T细胞的比例在ConA（SEB~+ConA组）或SEB（SEB~+SEB组）刺激后均较PBS组（PBS~+ConA组；PBS~+SEB组）的明显减少(P0.01或P0.05)；而SEB组CD4~+CD8~+T细胞的比例较PBS组明显增加(P0.01或P0.05)，但胸腺中CD4-CD8-T细胞的比例在两组间无统计学差异。 4.2妊娠期母鼠接触SEB对体外培养新生鼠胸腺中TCR Vβ8.2~+T细胞的影响 PBS组和SEB组新生鼠胸腺体外与ConA或SEB共培养三天后，SEB组的CD4~+TCRVβ8.2~+T细胞和CD8~+TCR Vβ8.2~+T细胞比例均较PBS组明显减少(P0.01或P0.05)。 5.妊娠期母鼠接触SEB对新生鼠脾脏淋巴细胞体外增殖的影响 5.1妊娠期母鼠接触SEB对新生鼠脾脏T淋巴细胞亚群增殖的影响 PBS组和SEB组新生鼠脾脏淋巴细胞体外与ConA或SEB共培养三天后，SEB组CD4~+T细胞比例在ConA或SEB刺激第一天分别较PBS组明显增加，但在第2-3天却明显降低(P0.01或P0.05)，但两组的CD8~+T细胞比例无统计学差异。而SEB组新生鼠脾脏淋巴细胞中CD4~+TCR Vβ8.2~+T细胞比例在ConA或SEB刺激的第1天及CD8~+TCR Vβ8.2~+T细胞比例在连续刺激三天分别较PBS组的明显增加(P0.01或P0.05)，但两组CD4~+TCR Vβ8.2~+T细胞比例在ConA或SEB刺激的第2-3天无差异性。 5.2妊娠期母鼠接触SEB对新生鼠脾脏淋巴细胞增殖指数的影响 PBS组和SEB组新生鼠脾脏淋巴细胞体外与ConA或SEB共培养三天后，，3H掺入法检测结果发现SEB组新生鼠脾脏淋巴细胞在ConA或SEB刺激后第1天增殖能力明显强于PBS组，但在第2-3天的增殖能力却又明显低于PBS组(P0.01或P0.05)。 结论： 1.妊娠期母鼠接触SEB可明显影响胎鼠胸腺及脾脏的发育； 2.妊娠期母鼠接触SEB可通过中枢和外周两种克隆清除方式导致新生鼠TCRVβ8.2~+T细胞比例减少，但SEB并非仅仅作用于TCR Vβ8.2~+T细胞，可能对其它的T淋巴细胞也产生影响； 3.在受到抗原刺激时，妊娠期母鼠接触SEB并不影响新生鼠胸腺中DN细胞向DP细胞的发育过程，但明显减少DP细胞向SP细胞的成熟过程； 4. CD4~+T细胞可能是妊娠期接触SEB母鼠的新生鼠脾脏淋巴细胞增殖的主要细胞群体； 5.妊娠期母鼠接触SEB已在新生儿期对新生鼠CD4~+T细胞、CD8~+T细胞以及TCRVβ8.2~+T细胞形成印迹效应，可能成为胎源性疾病的起源。
[Abstract]:Objective:
Staphylococcal enterotoxin B (SEB) is not only a toxic substance, but also the most important and widely studied superantigen in the nine serotypes of the staphylococcal enterotoxin family. As a superantigen, SEB can directly present cells by bridging antigen without recognising and processing antigen presenting cells. MHC class II molecules bind to the V beta region of the T lymphocyte TCR to stimulate the activation of a large number of T cells in the body's immune system. Usually SEB enters the body, initially, it can activate V beta 8~+ T cells and induce a large number of proliferation, and then apoptosis of overproliferated cells leads to clonal clearance, resulting in central or peripheral tolerance. The effect of SEB on immune organs, T lymphocytes and cytokines of adult and newborn mice has not been reported at home and abroad, but the effect of maternal exposure to SEB during pregnancy on cellular immunity of offspring newborn mice has not been reported at present. CD4/CD8T cells and TCR V-beta 8~+ T cell subsets in thymus, spleen and peripheral blood were detected at day 0-5, and the differentiation and development of thymocytes stimulated by ConA or SEB were observed in vitro in neonatal rats.
Method:
1. Choose clean adult SD rats, after mating and definite conception, the pregnant rats were fed to 16 days of gestation and divided into 12 groups randomly. The pregnant rats in the experimental group (SEB group) were injected with 15 UG SEB through the caudal vein, and the control group was set up to give the same volume of PBS (phosphate buffer).
2. At the end of pregnancy (21 days), the pregnant rats were anesthetized with 4% chloral hydrate, then the abdominal cavity and uterus were opened, the fetal rats were removed and the thymus and spleen were separated. The dry and wet weight of the fetus, placenta, thymus and spleen were weighed.
3. The thymus, spleen and peripheral blood of each group of newborn rats were obtained on the 0th to 5th day after birth. The single cell suspension of thymus and spleen was prepared by mesh rubbing method. The single cell suspension of spleen and peripheral blood were removed by erythrocyte lysate. The cell suspension was divided into two parts, one with fluorescent antibody CD3-FITC, CD4-APC and CD8-PE. Cell staining was performed, and the other was stained with V-beta 8.2-FITC, CD4-APC and CD8-PE. The ratio of T cell subsets was detected by flow cytometry.
4. The thymus of two groups of newborn mice were cultured in vitro by thymus organ culture (TOC) and added ConA or SEB for three days. At the end of culture, the thymus was collected and the single cell suspension was prepared. The ratio of T cell subsets in the thymus was detected by flow cytometry after staining.
5. Splenic lymphocytes were isolated from the spleens of two groups of newborn mice on the third day after birth and co-cultured with ConA or SEB for three consecutive days in vitro. At the end of the first, second and third days of culture, splenic lymphocytes were collected and stained, and the proportion of T cell subsets in the spleen was detected by flow cytometry. At the end of each time point, 3H-TDR was added 5 hours before the end of each time point, and then rpm was detected by gamma counter.
Result:
1. the influence of SEB on weight of fetal rats during pregnancy.
The wet weight and dry weight of thymus and spleen were significantly reduced after intravenous injection of SEB on the 16th day of gestation (P 0.01), but there was no significant difference between SEB group and PBS group.
2.1 the effect of SEB exposure on CD4/CD8T cell subsets in thymus of neonatal rats during gestation period
The proportion of CD4~+ T cells in the thymus of SEB group was significantly higher than that of PBS group (P 0.01 or P 0.05) on the day 0-5 after birth, and that of CD8~+ T cells in SEB group was significantly lower than that of PBS group (P 0.01 or P 0.05).
2.2 the effect of SEB exposure on CD4/CD8T cell subsets in spleen of neonatal rats during gestation period
The ratio of CD4~+T cells in spleen of SEB group was significantly higher than that of PBS group at each time point (P 0.01 or P 0.05) at 0-5 days after birth, but there was no significant difference between the two groups at each time point.
2.3 the effect of SEB exposure on CD4/CD8T cell subsets in peripheral blood of neonatal rats during gestation period
The percentage of CD4~+ T cells in peripheral blood and CD8~+ T cells in the PBS and SEB groups was similar to that in the spleen at day 0-5 after birth. The ratio of 8T cells was significantly higher than that of group PBS (P0.01 or P0.05).
3. the effect of SEB exposure on TCR and V 8.2~+T cells in neonatal rats during pregnancy
The proportion of CD4~+TCR V beta 8.2~+ T cells in the thymus of SEB group was significantly lower than that of PBS group (P 0.01 or P 0.05) on day 16 of gestation, and the proportion of CD4~+TCR V beta 8.2~+ T cells in the thymus of SEB group tended to be stable on day 4-5 of gestation, and there was no statistical difference between the two groups. The proportion of CD8~+TCR V beta 8.2~+ T cells in thymus and peripheral blood and the proportion of CD4~+TCR V beta 8.2~+ T cells in peripheral blood were significantly lower than those in PBS group (P 0.01 or P 0.05).
4. the effect of SEB exposure on the differentiation and development of thymus in neonatal rats in gestation period
4.1 the effect of SEB exposure on CD4/CD8T cell subsets in neonatal rat thymus during pregnancy
Three days after co-culture with ConA or SEB in vitro, the proportion of CD4~+ T cells and CD8~+ T cells in the thymus of PBS group and SEB group was significantly lower than that of PBS group (PBS + ConA group; PBS + SEB group) after stimulation by ConA (SEB + ConA group) or SEB (SEB + SEB group). Significantly increased (P0.01 or P0.05), but the proportion of CD4-CD8-T cells in thymus was not significantly different between the two groups.
4.2 the effect of SEB exposure on TCR V beta 8.2~+T cells in the thymus of neonatal rats in gestation period
Three days after co-culture with ConA or SEB in vitro, the proportion of CD4~+TCRV beta 8.2~+ T cells and CD8~+TCR V beta 8.2~+ T cells in SEB group were significantly lower than that in PBS group (P 0.01 or P 0.05).
5. the effect of SEB exposure on the proliferation of splenic lymphocytes in neonatal rats in gestation period
5.1 the effect of SEB exposure on the proliferation of T lymphocyte subsets in spleen of neonatal rats during gestation period
Three days after co-culture with ConA or SEB in vitro, the ratio of CD4~+ T cells in the spleen lymphocytes of PBS and SEB groups increased significantly on the first day of ConA or SEB stimulation, but decreased significantly on the second and third days (P 0.01 or P 0.05), but there was no significant difference in the ratio of CD8~+ T cells between the two groups. The ratio of TCR V-beta 8.2~+ T cells on the first day of ConA or SEB stimulation and the ratio of CD8~+TCR V-beta 8.2~+ T cells on the third day of continuous stimulation were significantly higher than that of PBS group (P 0.01 or P 0.05), but the ratio of CD4~+TCR V-beta 8.2~+ T cells on the second and third days of ConA or SEB stimulation had no difference.
5.2 the effect of SEB exposure on the proliferation index of spleen lymphocytes in neonatal rats during gestation period
Three days after co-culture with ConA or SEB in vitro, 3H incorporation assay showed that the proliferation of splenic lymphocytes in SEB group was significantly stronger than that in PBS group on the first day after ConA or SEB stimulation, but significantly lower than that in PBS group on the second and third days (P 0.01 or P 0.05).
Conclusion:
1. exposure to SEB in pregnant rats can significantly affect the development of thymus and spleen in fetal rats.
2. Exposure to SEB during pregnancy can reduce the proportion of TCRV-beta 8.2~+ T cells in neonatal rats through central and peripheral clonal clearance. However, SEB does not only affect TCR-V-beta 8.2~+ T cells, but may also affect other T lymphocytes.
3. Exposure to SEB during pregnancy did not affect the development of DN cells into DP cells, but significantly reduced the maturation of DP cells into SP cells.
4. CD4~+T cells may be the main cell population of splenic lymphocyte proliferation in neonatal rats exposed to SEB during pregnancy.
5. The imprinting effect of SEB exposure on neonatal rat CD4~+ T cells, CD8~+ T cells and TCRV beta 8.2~+ T cells may be the origin of fetal diseases.
【学位授予单位】：蚌埠医学院
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R378.11
本文编号：2199088
[Abstract]:Objective:
Staphylococcal enterotoxin B (SEB) is not only a toxic substance, but also the most important and widely studied superantigen in the nine serotypes of the staphylococcal enterotoxin family. As a superantigen, SEB can directly present cells by bridging antigen without recognising and processing antigen presenting cells. MHC class II molecules bind to the V beta region of the T lymphocyte TCR to stimulate the activation of a large number of T cells in the body's immune system. Usually SEB enters the body, initially, it can activate V beta 8~+ T cells and induce a large number of proliferation, and then apoptosis of overproliferated cells leads to clonal clearance, resulting in central or peripheral tolerance. The effect of SEB on immune organs, T lymphocytes and cytokines of adult and newborn mice has not been reported at home and abroad, but the effect of maternal exposure to SEB during pregnancy on cellular immunity of offspring newborn mice has not been reported at present. CD4/CD8T cells and TCR V-beta 8~+ T cell subsets in thymus, spleen and peripheral blood were detected at day 0-5, and the differentiation and development of thymocytes stimulated by ConA or SEB were observed in vitro in neonatal rats.
Method:
1. Choose clean adult SD rats, after mating and definite conception, the pregnant rats were fed to 16 days of gestation and divided into 12 groups randomly. The pregnant rats in the experimental group (SEB group) were injected with 15 UG SEB through the caudal vein, and the control group was set up to give the same volume of PBS (phosphate buffer).
2. At the end of pregnancy (21 days), the pregnant rats were anesthetized with 4% chloral hydrate, then the abdominal cavity and uterus were opened, the fetal rats were removed and the thymus and spleen were separated. The dry and wet weight of the fetus, placenta, thymus and spleen were weighed.
3. The thymus, spleen and peripheral blood of each group of newborn rats were obtained on the 0th to 5th day after birth. The single cell suspension of thymus and spleen was prepared by mesh rubbing method. The single cell suspension of spleen and peripheral blood were removed by erythrocyte lysate. The cell suspension was divided into two parts, one with fluorescent antibody CD3-FITC, CD4-APC and CD8-PE. Cell staining was performed, and the other was stained with V-beta 8.2-FITC, CD4-APC and CD8-PE. The ratio of T cell subsets was detected by flow cytometry.
4. The thymus of two groups of newborn mice were cultured in vitro by thymus organ culture (TOC) and added ConA or SEB for three days. At the end of culture, the thymus was collected and the single cell suspension was prepared. The ratio of T cell subsets in the thymus was detected by flow cytometry after staining.
5. Splenic lymphocytes were isolated from the spleens of two groups of newborn mice on the third day after birth and co-cultured with ConA or SEB for three consecutive days in vitro. At the end of the first, second and third days of culture, splenic lymphocytes were collected and stained, and the proportion of T cell subsets in the spleen was detected by flow cytometry. At the end of each time point, 3H-TDR was added 5 hours before the end of each time point, and then rpm was detected by gamma counter.
Result:
1. the influence of SEB on weight of fetal rats during pregnancy.
The wet weight and dry weight of thymus and spleen were significantly reduced after intravenous injection of SEB on the 16th day of gestation (P 0.01), but there was no significant difference between SEB group and PBS group.
2.1 the effect of SEB exposure on CD4/CD8T cell subsets in thymus of neonatal rats during gestation period
The proportion of CD4~+ T cells in the thymus of SEB group was significantly higher than that of PBS group (P 0.01 or P 0.05) on the day 0-5 after birth, and that of CD8~+ T cells in SEB group was significantly lower than that of PBS group (P 0.01 or P 0.05).
2.2 the effect of SEB exposure on CD4/CD8T cell subsets in spleen of neonatal rats during gestation period
The ratio of CD4~+T cells in spleen of SEB group was significantly higher than that of PBS group at each time point (P 0.01 or P 0.05) at 0-5 days after birth, but there was no significant difference between the two groups at each time point.
2.3 the effect of SEB exposure on CD4/CD8T cell subsets in peripheral blood of neonatal rats during gestation period
The percentage of CD4~+ T cells in peripheral blood and CD8~+ T cells in the PBS and SEB groups was similar to that in the spleen at day 0-5 after birth. The ratio of 8T cells was significantly higher than that of group PBS (P0.01 or P0.05).
3. the effect of SEB exposure on TCR and V 8.2~+T cells in neonatal rats during pregnancy
The proportion of CD4~+TCR V beta 8.2~+ T cells in the thymus of SEB group was significantly lower than that of PBS group (P 0.01 or P 0.05) on day 16 of gestation, and the proportion of CD4~+TCR V beta 8.2~+ T cells in the thymus of SEB group tended to be stable on day 4-5 of gestation, and there was no statistical difference between the two groups. The proportion of CD8~+TCR V beta 8.2~+ T cells in thymus and peripheral blood and the proportion of CD4~+TCR V beta 8.2~+ T cells in peripheral blood were significantly lower than those in PBS group (P 0.01 or P 0.05).
4. the effect of SEB exposure on the differentiation and development of thymus in neonatal rats in gestation period
4.1 the effect of SEB exposure on CD4/CD8T cell subsets in neonatal rat thymus during pregnancy
Three days after co-culture with ConA or SEB in vitro, the proportion of CD4~+ T cells and CD8~+ T cells in the thymus of PBS group and SEB group was significantly lower than that of PBS group (PBS + ConA group; PBS + SEB group) after stimulation by ConA (SEB + ConA group) or SEB (SEB + SEB group). Significantly increased (P0.01 or P0.05), but the proportion of CD4-CD8-T cells in thymus was not significantly different between the two groups.
4.2 the effect of SEB exposure on TCR V beta 8.2~+T cells in the thymus of neonatal rats in gestation period
Three days after co-culture with ConA or SEB in vitro, the proportion of CD4~+TCRV beta 8.2~+ T cells and CD8~+TCR V beta 8.2~+ T cells in SEB group were significantly lower than that in PBS group (P 0.01 or P 0.05).
5. the effect of SEB exposure on the proliferation of splenic lymphocytes in neonatal rats in gestation period
5.1 the effect of SEB exposure on the proliferation of T lymphocyte subsets in spleen of neonatal rats during gestation period
Three days after co-culture with ConA or SEB in vitro, the ratio of CD4~+ T cells in the spleen lymphocytes of PBS and SEB groups increased significantly on the first day of ConA or SEB stimulation, but decreased significantly on the second and third days (P 0.01 or P 0.05), but there was no significant difference in the ratio of CD8~+ T cells between the two groups. The ratio of TCR V-beta 8.2~+ T cells on the first day of ConA or SEB stimulation and the ratio of CD8~+TCR V-beta 8.2~+ T cells on the third day of continuous stimulation were significantly higher than that of PBS group (P 0.01 or P 0.05), but the ratio of CD4~+TCR V-beta 8.2~+ T cells on the second and third days of ConA or SEB stimulation had no difference.
5.2 the effect of SEB exposure on the proliferation index of spleen lymphocytes in neonatal rats during gestation period
Three days after co-culture with ConA or SEB in vitro, 3H incorporation assay showed that the proliferation of splenic lymphocytes in SEB group was significantly stronger than that in PBS group on the first day after ConA or SEB stimulation, but significantly lower than that in PBS group on the second and third days (P 0.01 or P 0.05).
Conclusion:
1. exposure to SEB in pregnant rats can significantly affect the development of thymus and spleen in fetal rats.
2. Exposure to SEB during pregnancy can reduce the proportion of TCRV-beta 8.2~+ T cells in neonatal rats through central and peripheral clonal clearance. However, SEB does not only affect TCR-V-beta 8.2~+ T cells, but may also affect other T lymphocytes.
3. Exposure to SEB during pregnancy did not affect the development of DN cells into DP cells, but significantly reduced the maturation of DP cells into SP cells.
4. CD4~+T cells may be the main cell population of splenic lymphocyte proliferation in neonatal rats exposed to SEB during pregnancy.
5. The imprinting effect of SEB exposure on neonatal rat CD4~+ T cells, CD8~+ T cells and TCRV beta 8.2~+ T cells may be the origin of fetal diseases.
【学位授予单位】：蚌埠医学院
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R378.11
【参考文献】
相关期刊论文 前2条
1 管俊昌,夏佩莹,唐素兰;金黄色葡萄球菌L型产B型肠毒素及其基因的研究[J];蚌埠医学院学报;2004年04期
2 管俊昌,刘勇,徐飞,夏佩莹;金葡菌L型对小鼠体外胚胎发育的影响[J];中国人兽共患病杂志;2004年11期
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