白念珠菌CaGUA1基因的功能研究
发布时间：2018-08-28 12:23
【摘要】： 鸟嘌呤核苷酸参与细胞内许多重要的生命过程,对DNA复制、mRNA转录和GTP蛋白介导的信号传导途径等重要的细胞活动起到关键作用。白念珠菌CaGUA1基因(orf19.4813,orf19.12276 )的ORF全长1593bp,编码一个含530个氨基酸残基的蛋白,该蛋白与ScGUA1编码蛋白有84%同源性。本实验中,我们使用了一种名为“URA-BLASTER”的基因敲除方法构建了白念珠菌CaGUA1基因的缺失株(gua1△/gua1△),该缺失株在无外源鸟嘌呤时无法存活。然后我们将CaGUA1基因克隆到白念珠菌的表达载体pCR4中,接着我们将该基因克隆转化到CaGUA1基因的缺失株中。含有pCR4-CaGUA1的缺失株在没有外源鸟嘌呤的条件下可以正常生长,表明CaGUA1基因是白念珠菌的鸟嘌呤合成途径中的必需基因。
[Abstract]:Guanine nucleotides are involved in many important cellular processes and play a key role in the transcription of DNA replication mRNA and the signaling pathway mediated by GTP protein. The ORF of CaGUA1 gene (orf19.4813,orf19.12276) of Candida albicans is 1593bp. it encodes a protein containing 530 amino acid residues. The protein shares 84% homology with the protein encoded by ScGUA1. In this study, we used a gene knockout method called "URA-BLASTER" to construct a CaGUA1 deletion strain of Candida albicans (gua1 / gua1), which could not survive without exogenous guanine. Then we cloned the CaGUA1 gene into the expression vector pCR4 of Candida albicans, and then we cloned the gene into the missing strain of CaGUA1 gene. The deletion strain containing pCR4-CaGUA1 can grow normally without exogenous guanine, indicating that CaGUA1 gene is an essential gene in the guanine synthesis pathway of Candida albicans.
【学位授予单位】：天津大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R378
本文编号：2209386
[Abstract]:Guanine nucleotides are involved in many important cellular processes and play a key role in the transcription of DNA replication mRNA and the signaling pathway mediated by GTP protein. The ORF of CaGUA1 gene (orf19.4813,orf19.12276) of Candida albicans is 1593bp. it encodes a protein containing 530 amino acid residues. The protein shares 84% homology with the protein encoded by ScGUA1. In this study, we used a gene knockout method called "URA-BLASTER" to construct a CaGUA1 deletion strain of Candida albicans (gua1 / gua1), which could not survive without exogenous guanine. Then we cloned the CaGUA1 gene into the expression vector pCR4 of Candida albicans, and then we cloned the gene into the missing strain of CaGUA1 gene. The deletion strain containing pCR4-CaGUA1 can grow normally without exogenous guanine, indicating that CaGUA1 gene is an essential gene in the guanine synthesis pathway of Candida albicans.
【学位授予单位】：天津大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R378
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