离体兔肺灌注模型的建立及不同成分保护液的肺保护研究

发布时间：2018-08-31 15:40

【摘要】： 【目的】:建立离体兔肺灌注模型,研究UW液、硝酸甘油、前列腺素以及自体血液等不同成分保存液对离体兔肺的保护作用。【材料和方法】:将30只新西兰大白兔分为5实验组,分别为对照组单纯UW液(A组)、UW液+硝酸甘油组(B组)、UW液+前列腺素E1(C组)、UW液与自身动脉血1:2混合组(D组)、UW液与自身动脉血1:4混合组(E组)。切取实验兔整体心肺作为离体心肺模型,对心肺模型进行冷灌注、低温保存。分别在2h末和4h末,取肺部分标本做电镜分析。于4h末,结扎右肺门,取右肺检测肺湿干重比、灌注前后肺气道压力变化。剩余离体肺接呼吸机模拟移植手术后呼吸,并将自身静脉血用泵从主肺动脉泵入,用负压吸引真空试管取左心房血液做血气分析。【结果】:实验E、D组对于肺组织气体交换功能的保护无明显差别,与B、C、A组相比有明显优势。实验B、C组对于肺实质细胞的气体交换功能的保护结果相当,明显优于对照A组。肺湿干重比、灌注前后肺气道压力变化结果显示实验B、C组优于实验D、E组及对照A组。【结论】:本实验离体心肺模型可靠,稳定性好。在UW液中加入自体动脉血对离体肺灌注可以使肺保存时间明显延长、肺气体交换功能保存良好,明显优于其他实验组和对照组。在UW液中加入前列腺素E1和硝酸甘油对离体肺保护作用再次得到肯定,其对肺气体交换功能的保护明显好于对照组,但和自体动脉血组相比仍有不足。在防止肺间质水肿和肺细胞水肿方面结果显示硝酸甘油和前列腺素E1组的作用相当,明显好于自体动脉血组和对照组。
[Abstract]:[objective]: to establish an isolated rabbit lung perfusion model and to study UW solution and nitroglycerin. The protective effects of prostaglandin and autologous blood on rabbit lung in vitro. [materials and methods]: thirty New Zealand white rabbits were divided into 5 experimental groups. UW solution (group A), nitroglycerin solution (group B), prostaglandin E1 (group C), UW solution and autogenous arterial blood (group D) were mixed with UW solution and auto-arterial blood 1:4 (group E). The whole heart and lung of experimental rabbits were taken as isolated cardiopulmonary model. Cold perfusion and cryopreservation were performed on the heart and lung model. At the end of 2 h and 4 h, the lung specimens were taken for electron microscopic analysis. At the end of 4 h, the right lung was ligated and the lung wet / dry weight ratio was measured. The left isolated lung was followed by a ventilator to simulate the respiration after transplantation, and the venous blood was pumped in from the main pulmonary artery with a pump. The blood of left atrium was obtained by vacuum tube suction with negative pressure for blood gas analysis. [results]: there was no significant difference in the protection of lung tissue gas exchange function between group E and D, and there were obvious advantages compared with group A. The gas exchange function of lung parenchyma cells in group B C was significantly better than that in group A. The results of lung wet / dry weight ratio and pulmonary duct pressure before and after perfusion showed that the experimental group BX C was superior to experimental group E and control group. [conclusion]: the isolated cardiopulmonary model of this experiment is reliable and stable. The addition of autologous arterial blood to the UW solution could prolong the lung preservation time and preserve the lung gas exchange function better than the other experimental group and control group. The protective effect of prostaglandin E1 and nitroglycerin on lung in vitro was confirmed again in UW solution. The protective effect of prostaglandin E1 and nitroglycerin on lung gas exchange function was better than that in control group, but it was still insufficient compared with autologous arterial blood group. The results showed that nitroglycerin and prostaglandin E1 had the same effect on preventing pulmonary interstitial edema and lung cell edema, which was better than that of autologous arterial blood group and control group.
【学位授予单位】：南京医科大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R-332;R655.3

【相似文献】