PBC患者肝内和外周血淋巴细胞CCR5、CXCR3、CXCR6和CXCR7的表达及意义
发布时间：2018-09-03 09:11
【摘要】： 目的比较原发性胆汁性肝硬化(primary biliary cirrhosis,PBC)患者肝内和外周血淋巴细胞上趋化因子受体CCR5、CXCR3、CXCR6和CXCR7的表达水平,及其与肝脏组织学炎症反应的关系,在趋化因子及其受体领域上初步探讨原发性胆汁性肝硬化的发病机制。 方法本研究同时收集来自昆明医学院第二附属医院肝胆胰内科2008年2月～2008年12月住院并确诊的11例PBC患者肝组织活检标本和静脉血样本。应用Beckman Coulter Epics XL流式细胞仪检测肝组织和外周血淋巴细胞CCR5、CXCR3、CXCR6和CXCR7的表达情况,并进行对比分析。 结果1、一般资料原发性胆汁性肝硬化患者11例,平均年龄43.3±14.6岁,女7例,男4例。其中原发性胆汁性肝硬化达Ⅲ-Ⅳ期和Ⅳ期共7例,Ⅱ期及以下为4例。 2、肝组织浸润淋巴细胞以CD8+、CD4+T淋巴细胞为主;外周血中CD4+T淋巴细胞明显占优势。肝脏组织中CD8+T淋巴细胞高于外周血,而肝组织中B淋巴细胞与外周血比较无统计学差异(P＞0.05)。外周血中CD4+T淋巴细胞高于肝组织(P＜0.05)。而不同肝组织学炎症程度组之间上述淋巴细胞亚群浸润比较均无统计学差异(P＞0.05)。 3、肝组织内表达CCR5、CXCR3、CXCR6明显高于外周血(P＜0.001),CXCR7在肝组织内表达水平要明显低于CCR5、CXCR3和CXCR6,但其在肝组织内表达仍高于外周血(P＜0.05)。 4、肝脏病变程度严重组的肝组织内CXCR6表达水平要高于病变轻微组(P＜0.05),CCR5、CXCR3和CXCR7的表达水平无统计学差异(P＞0.05)。 5、肝组织和外周血表达细胞因子IL-2、IL-4、IL-5、IL-10、IFN-γ和TNF-α水平均明显增高,但后者在两组之间比较均无统计学差异(P＞0.05)。 结论1、PBC与机体免疫功能异常密切相关,并且CD8+T细胞、CD4+T细胞介导的免疫病理反应起比较重要的作用,可能是自身免疫反应中靶器官——胆管上皮细胞损伤的重要机制。 2、趋化因子受体CCR5、CXCR3、CXCR6可能介导T淋巴细胞向肝内定植,并参与PBC肝脏炎症的病理免疫学反应过程。 3、趋化因子受体CXCR6在介导T淋巴细胞向肝内定植,参与肝脏炎症的病理免疫学反应过程中可能发挥更为重要的作用。 4、CXCR7的介导趋化作用轻微,可能不是调节T淋巴细胞向肝内定植的主要相关趋化因子受体。 5、细胞因子特别是IL-2、IFN-γ可能参与PBC病理形成过程。
[Abstract]:Objective to compare the expression levels of chemokine receptor (CCR5,CXCR3,CXCR6) and chemokine receptor (CXCR7) in liver and peripheral blood lymphocytes of patients with primary biliary cirrhosis (primary biliary cirrhosis,PBC) and their relationship with liver histologic inflammation. To explore the pathogenesis of primary biliary cirrhosis in the field of chemokines and their receptors. Methods liver biopsy specimens and venous blood samples from 11 patients with PBC from February 2008 to December 2008 in Department of Hepatobiliary and Pancreatic Medicine of the second affiliated Hospital of Kunming Medical College were collected. Beckman Coulter Epics XL flow cytometry was used to detect the expression of CCR5,CXCR3,CXCR6 and CXCR7 in liver and peripheral blood lymphocytes. Results 1. There were 11 patients with primary biliary cirrhosis with an average age of 43.3 卤14.6 years, 7 females and 4 males. There were 7 cases of primary biliary cirrhosis in stage 鈪，
本文编号：2219534
[Abstract]:Objective to compare the expression levels of chemokine receptor (CCR5,CXCR3,CXCR6) and chemokine receptor (CXCR7) in liver and peripheral blood lymphocytes of patients with primary biliary cirrhosis (primary biliary cirrhosis,PBC) and their relationship with liver histologic inflammation. To explore the pathogenesis of primary biliary cirrhosis in the field of chemokines and their receptors. Methods liver biopsy specimens and venous blood samples from 11 patients with PBC from February 2008 to December 2008 in Department of Hepatobiliary and Pancreatic Medicine of the second affiliated Hospital of Kunming Medical College were collected. Beckman Coulter Epics XL flow cytometry was used to detect the expression of CCR5,CXCR3,CXCR6 and CXCR7 in liver and peripheral blood lymphocytes. Results 1. There were 11 patients with primary biliary cirrhosis with an average age of 43.3 卤14.6 years, 7 females and 4 males. There were 7 cases of primary biliary cirrhosis in stage 鈪，
本文编号：2219534
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