有氧运动对CUMS大鼠急应激暴露的保护作用
发布时间：2018-09-04 08:36
【摘要】： 目的通过观察有氧运动对慢性轻度不可预期性应激(chronic unpredictedmild stress,CUMS)大鼠旷场行为、急应激暴露时血清皮质醇、白细胞介素-1β、海马与前额叶皮层热休克蛋白70和诱导型一氧化氮合酶表达的影响,探讨非药物性应对策略有氧运动对应激性神经、内分泌和免疫指标的影响。方法32只成年雄性Wistar大鼠随机分为对照组、CUMS组、拉贝洛尔组、有氧运动组,每组8只。CUMS组、拉贝洛尔组、有氧运动组采用21天慢性轻度不可预期性应激造模,造模过程中拉贝洛尔组大鼠在每次应激刺激前30min接受拉贝洛尔(30mg/kg)灌胃,有氧运动组大鼠进行无负重递增中等强度有氧游泳运动。所有动物在实验组造模结束后采用足底电击(1.5mA,电击5s休息55s,共60次)进行急应激暴露。以旷场实验评价大鼠行为学改变;以放射免疫法测定血清皮质醇和白细胞介素-1β含量;以免疫组织化学染色定位海马与前额叶皮层热休克蛋白70表达,蛋白免疫印迹法检测海马与前额叶皮层热休克蛋白70和诱导型一氧化氮合酶的表达。结果(1)与对照组相比,CUMS组大鼠中央格停留时间延长(P＜0.05),水平运动和垂直运动得分降低(P＜0.01;P＜0.01),清洁次数减少(P＜0.05);拉贝洛尔组中央格停留时间延长(P＜0.05),垂直运动得分降低(P＜0.01),清洁次数减少(P＜0.05),水平运动得分改善无统计学意义;有氧运动组各指标与对照组相比无显著性差异。与CUMS组比较,有氧运动组中央格停留时间缩短(P＜0.01),水平运动得分与垂直运动得分增加(P＜0.01;P＜0.01),清洁次数增加(P＜0.05)。拉贝洛尔组与有氧运动组相比中央格停留时间延长(P＜0.05),垂直运动得分降低(P＜0.01),清洁次数减少(P＜0.05)。(2)有氧运动组大鼠血清皮质醇含量较CUMS组、拉贝洛尔组升高,差异有统计学意义(P＜0.05;P＜0.05)。各组大鼠血清白细胞介素-1β水平均无统计学差异。(3)与CUMS模型组比,拉贝洛尔组前额叶皮层热休克蛋白70水平显著升高(P＜0.05),有氧运动组海马与前额叶皮层热休克蛋白70水平均显著升高(P＜0.01;P＜0.05)。且有氧运动组海马热休克蛋白70水平显著高于拉贝洛尔组(P＜0.05)。(4)CUMS组大鼠海马组织中诱导型一氧化氮合酶表达显著高于对照组(P＜0.01);与CUMS组相比,拉贝洛尔组海马与前额叶皮层诱导型一氧化氮合酶表达均显著降低(P＜0.05;P＜0.05);有氧运动组各脑区诱导型一氧化氮合酶表达水平与对照组比较无显著差异,但均显著低于CUMS组(P＜0.01;P＜0.01)。结论(1)CUMS可导致大鼠产生抑郁性行为,加重海马与前额叶皮层细胞应激损伤。(2)有氧运动可显著改善慢性轻度不可预期性应激大鼠行为学指标,稳定急应激暴露时下丘脑-垂体-肾上腺皮质轴的功能,通过显著增加海马与前额叶皮层热休克蛋白70表达、降低诱导型一氧化氮合酶表达增强机体应激抵抗力。(3)非选择性肾上腺素能受体拮抗剂拉贝洛尔可部分改善慢性轻度不可预期性应激大鼠行为学指标,对海马与前额叶皮层均有一定程度的保护作用。
[Abstract]:Objective To investigate the effects of aerobic exercise on open field behavior, serum cortisol, interleukin-1 beta, heat shock protein 70 (HSP70) and inducible nitric oxide synthase (INNOS) expression in the hippocampus and prefrontal cortex of chronic unpredictable mild stress (CUMS) rats, and explore the non-drug coping strategies of aerobic exercise. Methods Thirty-two adult male Wistar rats were randomly divided into control group, CUMS group, labetalol group and aerobic exercise group, with 8 rats in each group. Rats in the aerobic exercise group were given moderate-intensity aerobic swimming without increasing load 30 minutes before the second stress stimulation. All the rats in the experimental group were exposed to acute stress by plantar electric shock (1.5mA, 5 s rest for 55 seconds, 60 times). The levels of serum cortisol and interleukin-1 beta were determined by epidemic method, the expression of heat shock protein 70 in hippocampus and prefrontal cortex was localized by immunohistochemical staining, and the expression of heat shock protein 70 and inducible nitric oxide synthase in hippocampus and prefrontal cortex were detected by Western blotting. Results (1) Compared with the control group, the rats in CUMS group were treated with central gating. The retention time was prolonged (P < 0.05), the scores of horizontal and vertical movement were decreased (P < 0.01; P < 0.01), the cleaning times were decreased (P < 0.05), the stay time of central grid was prolonged (P < 0.05), the score of vertical movement was decreased (P < 0.01), the cleaning times were decreased (P < 0.05), and the scores of horizontal movement were not significantly improved in aerobic exercise group. Compared with the control group, the staying time of the central grid was shortened (P < 0.01), the scores of horizontal and vertical movement were increased (P < 0.01, P < 0.01), and the cleaning times were increased (P < 0.05) in the aerobic exercise group. The levels of serum cortisol in aerobic exercise group were significantly higher than those in CUMS group and Labellol group (P < 0.05; P < 0.05). There was no significant difference in the levels of serum interleukin-1 beta in each group. (3) Compared with CUMS model group, the levels of heat shock protein 7 in prefrontal cortex in Labellol group were significantly higher than those in CUMS group and Labellol group (P < 0.05; P < 0.05). The level of HSP70 in hippocampus and prefrontal cortex of aerobic exercise group was significantly higher than that of labetalol group (P < 0.05). (4) The expression of inducible nitric oxide synthase in hippocampus of CUMS group was significantly higher than that of control group (P Compared with CUMS group, the expression of inducible nitric oxide synthase in hippocampus and prefrontal cortex of labetalol group decreased significantly (P (2) Aerobic exercise can significantly improve the behavioral indexes of Chronic Mild Unpredictable Stress rats, stabilize the function of hypothalamus-pituitary-adrenal cortex axis during acute stress exposure, and increase the expression of heat shock protein 70 in hippocampus and prefrontal cortex. (3) Non-selective adrenergic receptor antagonist labetalol can partly improve the behavioral parameters of Chronic Mild Unpredictable Stress Rats and protect the hippocampus and prefrontal cortex to a certain extent.
【学位授予单位】：山东大学
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：R363
本文编号：2221568
[Abstract]:Objective To investigate the effects of aerobic exercise on open field behavior, serum cortisol, interleukin-1 beta, heat shock protein 70 (HSP70) and inducible nitric oxide synthase (INNOS) expression in the hippocampus and prefrontal cortex of chronic unpredictable mild stress (CUMS) rats, and explore the non-drug coping strategies of aerobic exercise. Methods Thirty-two adult male Wistar rats were randomly divided into control group, CUMS group, labetalol group and aerobic exercise group, with 8 rats in each group. Rats in the aerobic exercise group were given moderate-intensity aerobic swimming without increasing load 30 minutes before the second stress stimulation. All the rats in the experimental group were exposed to acute stress by plantar electric shock (1.5mA, 5 s rest for 55 seconds, 60 times). The levels of serum cortisol and interleukin-1 beta were determined by epidemic method, the expression of heat shock protein 70 in hippocampus and prefrontal cortex was localized by immunohistochemical staining, and the expression of heat shock protein 70 and inducible nitric oxide synthase in hippocampus and prefrontal cortex were detected by Western blotting. Results (1) Compared with the control group, the rats in CUMS group were treated with central gating. The retention time was prolonged (P < 0.05), the scores of horizontal and vertical movement were decreased (P < 0.01; P < 0.01), the cleaning times were decreased (P < 0.05), the stay time of central grid was prolonged (P < 0.05), the score of vertical movement was decreased (P < 0.01), the cleaning times were decreased (P < 0.05), and the scores of horizontal movement were not significantly improved in aerobic exercise group. Compared with the control group, the staying time of the central grid was shortened (P < 0.01), the scores of horizontal and vertical movement were increased (P < 0.01, P < 0.01), and the cleaning times were increased (P < 0.05) in the aerobic exercise group. The levels of serum cortisol in aerobic exercise group were significantly higher than those in CUMS group and Labellol group (P < 0.05; P < 0.05). There was no significant difference in the levels of serum interleukin-1 beta in each group. (3) Compared with CUMS model group, the levels of heat shock protein 7 in prefrontal cortex in Labellol group were significantly higher than those in CUMS group and Labellol group (P < 0.05; P < 0.05). The level of HSP70 in hippocampus and prefrontal cortex of aerobic exercise group was significantly higher than that of labetalol group (P < 0.05). (4) The expression of inducible nitric oxide synthase in hippocampus of CUMS group was significantly higher than that of control group (P Compared with CUMS group, the expression of inducible nitric oxide synthase in hippocampus and prefrontal cortex of labetalol group decreased significantly (P (2) Aerobic exercise can significantly improve the behavioral indexes of Chronic Mild Unpredictable Stress rats, stabilize the function of hypothalamus-pituitary-adrenal cortex axis during acute stress exposure, and increase the expression of heat shock protein 70 in hippocampus and prefrontal cortex. (3) Non-selective adrenergic receptor antagonist labetalol can partly improve the behavioral parameters of Chronic Mild Unpredictable Stress Rats and protect the hippocampus and prefrontal cortex to a certain extent.
【学位授予单位】：山东大学
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：R363
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