多糖类提取物及MF59佐剂对流感灭活疫苗免疫增强作用研究
发布时间：2018-09-04 13:12
【摘要】：本论文由两部分组成：中药多糖类提取物对流感灭活疫苗的免疫增强作用研究；MF59佐剂于不同免疫途径下作流感灭活疫苗的佐剂效果比较研究。 (一)中药多糖类提取物对流感灭活疫苗的免疫增强作用研究 (1)枸杞多糖(LBP)、黄芪多糖(APS)对A／PR／8流感灭活疫苗的体液免疫应答作用研究。不同剂量的LBP (300μg、500μg和800μg)或APS(300μg和500μg),及100μg的氢氧化铝分别与A／PR／8疫苗混合,经i.p.或i.m.两条免疫途径共免疫小鼠两次,间隔三周。ELISA检测实验结果表明,LBP、APS无论经i.p.或i.m.免疫,均能明显提高小鼠的抗体滴度,且呈现一定的剂量依赖性；高剂量的LBP及APS佐剂效果与氢氧化铝相当。 (2)茯苓多糖(pachymaran)能否作为低剂量(0.015μg)或高剂量(1.5μg)A／PR／8流感灭活疫苗的佐剂,增强疫苗的免疫原性。不同剂量的pachymaran(200μg和100μg)或100μg的氢氧化铝与A／PR／8流感灭活疫苗经i.m.共免疫小鼠,一免后三周通过检测抗体水平、病毒攻击后小鼠的体重变化及保护率等几个方面检测其效果。结果如下： ①pachymaran对低剂量A／PR／8疫苗的佐剂效应 1000μg pachymaran IgG、IgG1、IgG2a抗体滴度均显著高于200μg pachymaran组和单独A／PR／8疫苗组；攻毒后明显降低了小鼠的体重丢失及肺部病毒量,提高小鼠的存活率,保护效果与氢氧化铝相当。 ②pachymaran对高剂量A／PR／8疫苗的佐剂效应 Pachymaran仍能提高小鼠的IgG.IgG1、IgG2a抗体滴度,效果却逊于铝佐剂组,然而病毒攻击后,其体重丢失率却与铝佐剂组差异不大。 (二)检测MF59于不同免疫途径下的佐剂效果。将MF59与A／PR／8疫苗混合,分别经i.p.、i.m.、i.v.和i.n.共免疫小鼠一次或两次,间隔三周,一免后四周或加强免疫后一周用致死量流感病毒攻击小鼠,检测小鼠血清抗体滴度、体重丢失率及保护率。结果表明i.p.优于i.m.;i.v.产生的抗体效价与i.m.相当,然而保护效果却逊于i.m.；i.n.最差。
[Abstract]:This paper consists of two parts: the study on the immune enhancement effect of Chinese medicine polysaccharides extract on inactivated influenza vaccine; the comparative study on the adjuvant effect of MF59 adjuvant on inactivated influenza vaccine under different immune routes. (1) study on the immune Enhancement of Polysaccharide extracts from Chinese Medicine on Influenza inactivated Vaccine (1) Humoral immune response of Lycium barbarum Polysaccharide (LBP), Astragalus Polysaccharide (APS) to A/PR/8 Influenza inactivated Vaccine. Different doses of LBP (300 渭 g, 500 渭 g and 800 渭 g) or APS (300 渭 g, 500 渭 g), and 100 渭 g aluminum hydroxide) were mixed with A/PR/8 vaccine, respectively. Or i.m. Mice were immunized twice with three weeks interval. The results of Elisa showed that LBP- APS could be immunized by i.p. Or i.m. The antibody titers of mice were significantly increased by immunization, and the antibody titers were in a dose-dependent manner. (2) whether Poria cocos polysaccharide (pachymaran) can be used as adjuvant of low dose (0.015 渭 g) or high dose (1.5 渭 g) A/PR/8 inactivated vaccine to enhance the immunogenicity of the vaccine. Different doses of pachymaran (200 渭 g and 100 渭 g) or 100 渭 g aluminum hydroxide and A/PR/8 inactivated vaccine were inoculated with i.m. Three weeks after immunization, the antibody level, the weight change and the protective rate of the mice after virus attack were detected. The results were as follows: the adjuvant effect of 1pachymaran on low dose A/PR/8 vaccine was significantly higher than that of 1000 渭 g pachymaran IgG,IgG1,IgG2a antibody in low dose A/PR/8 vaccine group and only A/PR/8 vaccine group. After attacking the virus, the weight loss and lung virus content of the mice were significantly reduced, and the survival rate of the mice was increased. The protective effect was similar to that of aluminum hydroxide. The adjuvant effect of 2pachymaran on high dose A/PR/8 vaccine Pachymaran could still increase the titer of IgG.IgG1,IgG2a antibody in mice, but the effect was inferior to that of aluminum adjuvant group, but after virus attack, The weight loss rate was not significantly different from that in the aluminum adjuvant group. (2) to detect the adjuvant effect of MF59 in different immune pathways. The mixture of MF59 and A/PR/8 vaccine was separated by I. P. And i.n. Mice were immunized once or twice for three weeks, four weeks after immunization or one week after booster immunization with lethal influenza virus. The titer of serum antibody, the rate of weight loss and the rate of protection were detected. The results showed that i.p. It is superior to I. The titer of antibody produced was higher than that of i.m. However, the protective effect was inferior to that of I. The worst.
【学位授予单位】：湖南师范大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R392
本文编号：2222204
[Abstract]:This paper consists of two parts: the study on the immune enhancement effect of Chinese medicine polysaccharides extract on inactivated influenza vaccine; the comparative study on the adjuvant effect of MF59 adjuvant on inactivated influenza vaccine under different immune routes. (1) study on the immune Enhancement of Polysaccharide extracts from Chinese Medicine on Influenza inactivated Vaccine (1) Humoral immune response of Lycium barbarum Polysaccharide (LBP), Astragalus Polysaccharide (APS) to A/PR/8 Influenza inactivated Vaccine. Different doses of LBP (300 渭 g, 500 渭 g and 800 渭 g) or APS (300 渭 g, 500 渭 g), and 100 渭 g aluminum hydroxide) were mixed with A/PR/8 vaccine, respectively. Or i.m. Mice were immunized twice with three weeks interval. The results of Elisa showed that LBP- APS could be immunized by i.p. Or i.m. The antibody titers of mice were significantly increased by immunization, and the antibody titers were in a dose-dependent manner. (2) whether Poria cocos polysaccharide (pachymaran) can be used as adjuvant of low dose (0.015 渭 g) or high dose (1.5 渭 g) A/PR/8 inactivated vaccine to enhance the immunogenicity of the vaccine. Different doses of pachymaran (200 渭 g and 100 渭 g) or 100 渭 g aluminum hydroxide and A/PR/8 inactivated vaccine were inoculated with i.m. Three weeks after immunization, the antibody level, the weight change and the protective rate of the mice after virus attack were detected. The results were as follows: the adjuvant effect of 1pachymaran on low dose A/PR/8 vaccine was significantly higher than that of 1000 渭 g pachymaran IgG,IgG1,IgG2a antibody in low dose A/PR/8 vaccine group and only A/PR/8 vaccine group. After attacking the virus, the weight loss and lung virus content of the mice were significantly reduced, and the survival rate of the mice was increased. The protective effect was similar to that of aluminum hydroxide. The adjuvant effect of 2pachymaran on high dose A/PR/8 vaccine Pachymaran could still increase the titer of IgG.IgG1,IgG2a antibody in mice, but the effect was inferior to that of aluminum adjuvant group, but after virus attack, The weight loss rate was not significantly different from that in the aluminum adjuvant group. (2) to detect the adjuvant effect of MF59 in different immune pathways. The mixture of MF59 and A/PR/8 vaccine was separated by I. P. And i.n. Mice were immunized once or twice for three weeks, four weeks after immunization or one week after booster immunization with lethal influenza virus. The titer of serum antibody, the rate of weight loss and the rate of protection were detected. The results showed that i.p. It is superior to I. The titer of antibody produced was higher than that of i.m. However, the protective effect was inferior to that of I. The worst.
【学位授予单位】：湖南师范大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R392
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