miR-33s通过靶向p38 MAPK负性调节LPS诱导的炎症反应

发布时间：2018-09-07 12:40

【摘要】：目的探讨miR-33s是否能够通过靶向p38 MAPK负性调节LPS诱导的炎症反应。方法体外培养人单核细胞株THP-1,分别将miR-33s的拟似物(mimic,25 nmol/L)和miR-33s的抑制剂(inhibitor,25 nmol/L)转染至THP-1细胞24 h。用浓度10.0 ng/m L的LPS诱导转染后的THP-1细胞24 h,分别采用Realtime RT-PCR和Western blot方法检测细胞miR-33s及p38MAPK蛋白表达,ELISA法检测THP-1细胞培养上清液中白细胞介素1β(IL-1β)、肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)的分泌量。结果 miR-33s mimic转染组p38 MAPK蛋白表达明显减少(P0.05),IL-1β、TNF-α、IL-6含量显著降低(P0.05)。miR-33s inhibitor转染组p38MAPK蛋白表达明显增加(P0.05),IL-1β、TNF-α、IL-6含量显著上升(P0.05)。结论 LPS能诱导THP-1细胞释放IL-1β、TNF-α、IL-6;miR-33s mimic抑制炎性细胞因子的分泌。miR-33s inhibitor促进促炎性细胞因子的分泌;miR-33s可能通过靶向结合p38 MAPK的3'末端非编码区来发挥它的负性调节炎症作用。
[Abstract]:Objective to investigate whether miR-33s can negatively regulate the inflammatory response induced by LPS by targeting p38 MAPK. Methods Human mononuclear cell line THP-1, was transfected into THP-1 cells for 24 h after transfection of miR-33s mimicry (mimic,25 nmol/L) and miR-33s inhibitor (inhibitor,25 nmol/L). After transfection of THP-1 cells induced by 10.0 ng/m L LPS for 24 h, the expression of miR-33s and p38MAPK protein in THP-1 cell culture supernatant was detected by Realtime RT-PCR and Western blot respectively. The levels of interleukin 1 尾 (IL-1 尾), tumor necrosis factor 伪 (TNF- 伪) and white blood cell (WBC) in the culture supernatant of THP-1 cells were detected by Elisa. Secretion of interleukin 6 (IL-6). Results the expression of p38 MAPK protein was significantly decreased in miR-33s mimic transfected group (P0.05). (P0.05). The expression of p38MAPK protein in MIR-33s inhibitor group was significantly increased (P0.05). Conclusion LPS can induce THP-1 cells to release IL-1 尾 -TNF- 伪 -TNF- 伪 IL-6mmiR-33s mimic to inhibit the secretion of inflammatory cytokines. MiR-33s inhibitor can promote the secretion of pro-inflammatory cytokines. MiR-33s may play a negative role in inflammatory regulation by targeting the 3'terminal noncoding region of p38 MAPK.
【作者单位】：南华大学附属第二医院麻醉科;
【基金】：国家自然科学基金青年科学基金(编号:81301625) 湖南省科技厅科技基金(编号:2013FJ6006)
【分类号】：R364.5

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