大鼠迷走神经背核电刺激与小肠电活动的相关性的解析
发布时间：2018-09-08 12:24
【摘要】： 目的: 1.建立大鼠迷走神经背核电刺激模型。 2.分析电刺激迷走神经背核条件下大鼠空肠电活动的变化。 3.观察电刺激迷走神经背核条件下的大鼠空肠微循环的变化。 4.探讨迷走神经背核兴奋引起的大鼠小肠生理机能的改变,从电生理和微循环方面对迷走神经背核在急性脑血管病引起的应激性溃疡中的作用提供重要的参考依据。 方法: 1.建立大鼠迷走神经背核电刺激模型:麻醉大鼠,固定于鼠脑立体定位仪上。根据大鼠脑立体定位图谱,利用立体定位仪准确定位迷走神经背核,于定位点利用牙科钻施行颅骨打孔术,用牙科粉固定刺激电极,建立迷走神经背核电刺激的大鼠模型。 2.大鼠空肠电活动计测:大鼠禁食12-18小时,常规麻醉,沿腹部正中打开腹腔,暴露空肠,将双针状电极插于浆膜下,两电极间距离大约为2mm,接通RM6240B型多道生理信号采集处理系统记录大鼠迷走神经背核电刺激前与刺激后的大鼠消化间期空肠电活动。 3.大鼠空肠微循环变化的计测:大鼠禁食12-18小时,常规麻醉,沿腹部正中打开腹腔,暴露空肠在10倍镜下按微血管分级原则选取直径10-30μm的微静脉,使用BI-2000微循环动态图像分析系统测量微静脉的血流速度并予以记录。 4.大鼠迷走神经背核电刺激后脑组织大体标本及核团电毁损标本的制备:大鼠迷走神经背核电刺激造模,实验结束后,在相应核团上通以阳极电压5.0 V,60 Hz,20-30 s。断头取脑,置入福尔马林溶液。2-3 d后,冷却切片检查定位是否准确。 5.统计学方法:采用SPSS13.0统计分析软件进行数据分析。大鼠迷走神经背核电刺激前后的空肠慢波变化及微循环血流速度的变化采用配对t检验分析;电刺激前和刺激1分钟内快波出现概率采用采用χ~2检验的Fisher's确切概率法进行分析,快波出现率的变化采用χ~2检验的方法进行分析。 结果: 1.通过大体标本、电毁损针道确定刺激部位正位于迷走神经背核。 2.比较大鼠迷走神经背核电兴奋引起的空肠电活动的变化。迷走神经背核电刺激后10分钟大鼠空肠慢波平均频率增加(P＜0.05),平均振幅增加(P＜0.05)。迷走神经背核电刺激1分钟内和刺激前1分钟相比,平均频率降低(P＜0.01),平均振幅增加(P＜0.01),快波发生概率增加(P＜0.05),快波出现率增加(P＜0.01)。 3.比较大鼠迷走神经背核电刺激前后空肠系膜微循环血流速度的变化,可见电刺激迷走神经背核后空肠系膜微血管血流速度加快(P＜0.01)。 结论: 1.利用脑立体定位技术可准确制作迷走神经背核电刺激的动物模型。 2.迷走神经背核电刺激后大鼠空肠电活动表现异常,主要表现为刺激即时动作电位高发,肠收缩运动增强;刺激后和刺激前相比慢波频率、振幅均增加。该结果提示,中枢神经系统损伤可能通过兴奋迷走神经背核进而对消化道平滑肌的功能活动产生影响,这种机制有可能通过支配消化道的神经电活动完成,最终会影响消化道的运动,成为应激性溃疡的神经电生理基础。 3.迷走神经背核电刺激后空肠系膜微循环血流速度加快。该结果提示,中枢神经系统损伤可能通过兴奋迷走神经背核,造成消化道粘膜微循环的紊乱。
[Abstract]:Objective:
1. a rat model of dorsal vagus nerve stimulation was established.
2. to analyze the changes of electrical activity of jejunum in rats under electrical stimulation of the dorsal vagal nucleus.
3. to observe the changes of jejunal microcirculation in rats under the condition of electrical stimulation of the dorsal vagal nucleus.
4. To explore the changes of intestinal physiological function induced by the excitation of dorsal vagal nucleus in rats, and to provide important reference for the role of dorsal vagal nucleus in stress ulcer induced by acute cerebrovascular disease from the aspects of electrophysiology and microcirculation.
Method:
1. Establish a rat model of vagal dorsal nucleus stimulation: anaesthetized rats, fixed on the stereotaxic apparatus of rat brain. According to the stereotaxic map of rat brain, the dorsal nucleus of vagus nerve was accurately located by stereotaxic apparatus. Rat model.
2. Measurement of jejunal electrical activity in rats: Rats were fasting for 12-18 hours, under general anesthesia, abdominal cavity was opened along the center of abdomen, jejunum was exposed, and double needle electrodes were inserted into the serosa. The distance between the two electrodes was about 2 mm. RM6240B multi-channel physiological signal acquisition and processing system was connected to record the digestive tract of rats before and after stimulation of dorsal vagal nucleus. Phase jejunal electrical activity.
3. Measurement of the changes of jejunal microcirculation in rats: Rats were fasting for 12-18 hours, under general anesthesia, the abdominal cavity was opened along the center of abdomen, and the jejunum was exposed under 10-fold microscope according to the principle of microvascular grading. The blood flow velocity of the venules was measured and recorded by BI-2000 microcirculation dynamic image analysis system.
4. The preparation of the general specimens and the electrolytic lesion specimens of the brain tissue after the stimulation of the dorsal vagal nucleus in rats: After the stimulation of the dorsal vagal nucleus in rats, the corresponding nuclei were exposed to the anodic voltage of 5.0 V, 60 Hz, 20-30 s.
5. Statistical methods: SPSS13.0 statistical software was used to analyze the data. The changes of jejunal slow wave and microcirculatory blood flow velocity before and after stimulation of dorsal vagal nucleus in rats were analyzed by paired t test. The occurrence probability of fast wave before and within 1 minute of stimulation was analyzed by Fisher's exact probability method using_~2 test. The occurrence rate of fast wave was analyzed by chi square ~2 test.
Result:
1. through the general specimen, electrical destruction of the needle path confirms that the stimulation site is located in the dorsal nucleus of the vagus nerve.
2. Comparing the changes of jejunal electrical activity induced by the excitation of the dorsal vagal nucleus in rats, the average frequency and amplitude of the slow wave of the jejunum increased (P < 0.05) and increased (P < 0.05) 10 minutes after the stimulation of the dorsal vagal nucleus. (P < 0.01), the incidence of fast wave increased (P < 0.05), and the incidence of fast wave increased (P < 0.01).
3. Comparing the changes of blood flow velocity of mesenteric microcirculation before and after stimulation of dorsal vagal nucleus in rats, it can be seen that the blood flow velocity of mesenteric microvasculature increased after stimulation of dorsal vagal nucleus (P < 0.01).
Conclusion:
1. using the stereotaxic technique, the animal model of dorsal vagal nucleus stimulation can be produced accurately.
2. The abnormal electrical activity of jejunum in rats after stimulation of dorsal vagal nucleus was mainly manifested by the high incidence of immediate action potential and the enhancement of intestinal contraction; the frequency and amplitude of slow wave increased after stimulation compared with that before stimulation. It is possible that this mechanism can be accomplished by innervating the nerve electrical activity of the digestive tract, eventually affecting the movement of the digestive tract and becoming the neuroelectrophysiological basis of stress ulcer.
3. The velocity of mesenteric microcirculation blood flow increased after stimulation of dorsal vagal nucleus. The results suggest that central nervous system injury may cause disturbance of gastrointestinal mucosal microcirculation by exciting dorsal vagal nucleus.
【学位授予单位】：天津医科大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R338
本文编号：2230510
[Abstract]:Objective:
1. a rat model of dorsal vagus nerve stimulation was established.
2. to analyze the changes of electrical activity of jejunum in rats under electrical stimulation of the dorsal vagal nucleus.
3. to observe the changes of jejunal microcirculation in rats under the condition of electrical stimulation of the dorsal vagal nucleus.
4. To explore the changes of intestinal physiological function induced by the excitation of dorsal vagal nucleus in rats, and to provide important reference for the role of dorsal vagal nucleus in stress ulcer induced by acute cerebrovascular disease from the aspects of electrophysiology and microcirculation.
Method:
1. Establish a rat model of vagal dorsal nucleus stimulation: anaesthetized rats, fixed on the stereotaxic apparatus of rat brain. According to the stereotaxic map of rat brain, the dorsal nucleus of vagus nerve was accurately located by stereotaxic apparatus. Rat model.
2. Measurement of jejunal electrical activity in rats: Rats were fasting for 12-18 hours, under general anesthesia, abdominal cavity was opened along the center of abdomen, jejunum was exposed, and double needle electrodes were inserted into the serosa. The distance between the two electrodes was about 2 mm. RM6240B multi-channel physiological signal acquisition and processing system was connected to record the digestive tract of rats before and after stimulation of dorsal vagal nucleus. Phase jejunal electrical activity.
3. Measurement of the changes of jejunal microcirculation in rats: Rats were fasting for 12-18 hours, under general anesthesia, the abdominal cavity was opened along the center of abdomen, and the jejunum was exposed under 10-fold microscope according to the principle of microvascular grading. The blood flow velocity of the venules was measured and recorded by BI-2000 microcirculation dynamic image analysis system.
4. The preparation of the general specimens and the electrolytic lesion specimens of the brain tissue after the stimulation of the dorsal vagal nucleus in rats: After the stimulation of the dorsal vagal nucleus in rats, the corresponding nuclei were exposed to the anodic voltage of 5.0 V, 60 Hz, 20-30 s.
5. Statistical methods: SPSS13.0 statistical software was used to analyze the data. The changes of jejunal slow wave and microcirculatory blood flow velocity before and after stimulation of dorsal vagal nucleus in rats were analyzed by paired t test. The occurrence probability of fast wave before and within 1 minute of stimulation was analyzed by Fisher's exact probability method using_~2 test. The occurrence rate of fast wave was analyzed by chi square ~2 test.
Result:
1. through the general specimen, electrical destruction of the needle path confirms that the stimulation site is located in the dorsal nucleus of the vagus nerve.
2. Comparing the changes of jejunal electrical activity induced by the excitation of the dorsal vagal nucleus in rats, the average frequency and amplitude of the slow wave of the jejunum increased (P < 0.05) and increased (P < 0.05) 10 minutes after the stimulation of the dorsal vagal nucleus. (P < 0.01), the incidence of fast wave increased (P < 0.05), and the incidence of fast wave increased (P < 0.01).
3. Comparing the changes of blood flow velocity of mesenteric microcirculation before and after stimulation of dorsal vagal nucleus in rats, it can be seen that the blood flow velocity of mesenteric microvasculature increased after stimulation of dorsal vagal nucleus (P < 0.01).
Conclusion:
1. using the stereotaxic technique, the animal model of dorsal vagal nucleus stimulation can be produced accurately.
2. The abnormal electrical activity of jejunum in rats after stimulation of dorsal vagal nucleus was mainly manifested by the high incidence of immediate action potential and the enhancement of intestinal contraction; the frequency and amplitude of slow wave increased after stimulation compared with that before stimulation. It is possible that this mechanism can be accomplished by innervating the nerve electrical activity of the digestive tract, eventually affecting the movement of the digestive tract and becoming the neuroelectrophysiological basis of stress ulcer.
3. The velocity of mesenteric microcirculation blood flow increased after stimulation of dorsal vagal nucleus. The results suggest that central nervous system injury may cause disturbance of gastrointestinal mucosal microcirculation by exciting dorsal vagal nucleus.
【学位授予单位】：天津医科大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R338
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