产后多虚多瘀动物模型的实验研究

发布时间：2018-09-13 15:47

【摘要】： 研究目的:通过对产后大鼠存在的气血亏虚、血瘀状态进行研究,论证此状态对机体生理、免疫等方面的影响,以论证中医关于“产后多虚多瘀”的理论,指导产褥期康复保健、预防产后病的发生,为产后病的临床研究和中药新药研究提供理想的动物模型。研究方法:将产后大鼠以游泳加放血方式制成“产后多虚多瘀”模型,与未孕正常组(正常组)、正常产后对照组(产后组)对照,检测产后第1天和第15天血常规、血流变的变化;各组大鼠外观表征的变化;肝脏、胸腺脏器系数。研究结果:1.大鼠产后第一天,红细胞、血红蛋白量、红细胞压积明显低于正常组(P＜0.01);第十五天,模型组RBC、HGB、HCT水平继续下降,与正常组及对照组比较有显著差异(P＜0.01),产后组已恢复到正常水平,与正常组无显著差异(P＞0.05)。2.模型组大鼠肝脏脏器系数明显升高,胸腺系数明显降低,与正常组比较有显著差异(P＜0.05)。3.大鼠产后存在气虚、血虚、血瘀的外观表征,模型组随着实验的进行,症状日渐明显,而产后组症状逐渐消退。结论:此种方法造模,在一定程度上可以模拟人体“产后多虚多瘀”的病理生理状态,可为产后病的研究提供良好动物模型。
[Abstract]:Objective: to study the state of deficiency of qi and blood stasis in postpartum rats, and to demonstrate the effect of this state on body physiology and immunity, so as to demonstrate the theory of "more deficiency and more blood stasis after delivery" in traditional Chinese medicine. To guide the puerperium rehabilitation and health care, to prevent postpartum disease, and to provide an ideal animal model for the clinical study of postpartum disease and the study of new drugs of traditional Chinese medicine. Methods: the postpartum rats were made into the model of "more deficiency and more blood stasis" by swimming and bleeding. The blood routine was detected on the 1st and 15th day of postpartum compared with normal group (normal group) and normal postpartum control group (postpartum group). Changes of hemorheology; changes in appearance of rats in each group; coefficient of liver and thymus organs. The result of the study was: 1. On the first day of postpartum, the contents of erythrocytes, hemoglobin, hematocrit in the model group were significantly lower than those in the normal group (P < 0. 01), and the level of RBC,HGB,HCT in the model group continued to decrease on the 15th day. There was significant difference between postpartum group and normal group (P < 0.01), but there was no significant difference between postpartum group and normal group (P > 0.05) .2. The liver organ coefficient and thymus coefficient in the model group were significantly higher than those in the normal group (P < 0.05) .3.The thymus coefficient of the model group was significantly lower than that of the normal group (P < 0.05). There were signs of deficiency of qi and blood stasis in postpartum rats. The symptoms of the model group became more and more obvious with the experiment, while the symptoms of the postpartum group gradually subsided. Conclusion: this method can simulate the pathophysiological state of postpartum "more deficiency and more blood stasis" to some extent, and can provide a good animal model for the study of postpartum disease.
【学位授予单位】：成都中医药大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R-332;R271

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