胆石六号汤剂对豚鼠胆色素结石模型胆囊的抗炎作用
发布时间：2018-10-10 15:39
【摘要】：目的探究胆石六号汤剂对胆色素结石豚鼠胆囊肿瘤坏死因子-α(TNF-α)及核转录因子(NF-κB)表达的影响。方法将雌性豚鼠40只随机分成3组,空白组10只,模型组15只,实验组15只。空白组除外,其余2组均采用饮食诱导法,建立胆色素结石模型。实验组,灌胃胆石六号汤剂7.7g·kg-1·d-1,其余2组均灌胃用同体积的0.9%氯化钠。30 d后,观察各组豚鼠成石率,用免疫组织化学技术检测胆囊TNF-α及NF-κB蛋白表达和胆囊病理组织的改变情况。结果模型组成石率为90.62%,明显高于空白组26.76%,表明造模成功;实验组成石率为11.00%,明显低于模型组90.62%,可见药物具有抗胆结石的作用。与空白组比较,模型组的TNF-α、NF-κB蛋白表达量明显增加(P0.01)。与模型组比较,实验组的TNF-α、NF-κB蛋白表达量明显较少(P0.05)。结论胆石六号汤剂可以明显减少胆色素结石豚鼠的成石率,这可能与药物降低胆色素结石豚鼠胆组织TNF-α、NF-κB蛋白的表达有关。
[Abstract]:Objective to investigate the effect of Ganshi No.6 decoction on the expression of tumor necrosis factor- 伪 (TNF- 伪) and nuclear transcription factor (NF- 魏 B) in gallbladder of pigmented gallstone guinea pigs. Methods 40 female guinea pigs were randomly divided into 3 groups: blank group (n = 10), model group (n = 15) and experimental group (n = 15). Except the blank group, the other two groups were induced by diet to establish the model of pigment gallstone. In the experimental group, 7. 7 g kg-1 d-1 of gavage gallstone no. 6 decoction, and 0. 9% sodium chloride in the other two groups were given intragastrically. 30 days later, the lithogenesis rate of guinea pigs in each group was observed, and the expression of TNF- 伪 and NF- 魏 B protein in gallbladder and the changes of gallbladder pathological tissue were detected by immunohistochemical technique. Results the rate of model stone composition was 90.62, which was significantly higher than that of blank group 26.76, which indicated that the model was successful, and the experimental composition stone rate was 11.00, which was significantly lower than that of model group 90.62. It can be seen that the drug has the effect of anti-gallstone. Compared with the blank group, the expression of TNF- 伪 and NF- 魏 B protein in the model group was significantly increased (P0.01). Compared with the model group, the expression of TNF- 伪 and NF- 魏 B protein in the experimental group was significantly lower (P0.05). Conclusion the gallstone formation rate of the guinea pigs with gallbladder pigment stone can be significantly reduced by the decoction. This may be related to the decrease of the expression of TNF- 伪 and NF- 魏 B protein in the gallbladder tissue of the guinea pigs with gallbladder pigment stone.
【作者单位】： 遵义医学院附属医院药剂科;遵义医学院药学院;
【基金】：贵州省教育厅自然科学基金资助项目[黔教合K字(2012)054号] 贵州省科学技术基金资助项目[黔科合J字(2008)215号]
【分类号】：R285.5;R-332
[Abstract]:Objective to investigate the effect of Ganshi No.6 decoction on the expression of tumor necrosis factor- 伪 (TNF- 伪) and nuclear transcription factor (NF- 魏 B) in gallbladder of pigmented gallstone guinea pigs. Methods 40 female guinea pigs were randomly divided into 3 groups: blank group (n = 10), model group (n = 15) and experimental group (n = 15). Except the blank group, the other two groups were induced by diet to establish the model of pigment gallstone. In the experimental group, 7. 7 g kg-1 d-1 of gavage gallstone no. 6 decoction, and 0. 9% sodium chloride in the other two groups were given intragastrically. 30 days later, the lithogenesis rate of guinea pigs in each group was observed, and the expression of TNF- 伪 and NF- 魏 B protein in gallbladder and the changes of gallbladder pathological tissue were detected by immunohistochemical technique. Results the rate of model stone composition was 90.62, which was significantly higher than that of blank group 26.76, which indicated that the model was successful, and the experimental composition stone rate was 11.00, which was significantly lower than that of model group 90.62. It can be seen that the drug has the effect of anti-gallstone. Compared with the blank group, the expression of TNF- 伪 and NF- 魏 B protein in the model group was significantly increased (P0.01). Compared with the model group, the expression of TNF- 伪 and NF- 魏 B protein in the experimental group was significantly lower (P0.05). Conclusion the gallstone formation rate of the guinea pigs with gallbladder pigment stone can be significantly reduced by the decoction. This may be related to the decrease of the expression of TNF- 伪 and NF- 魏 B protein in the gallbladder tissue of the guinea pigs with gallbladder pigment stone.
【作者单位】： 遵义医学院附属医院药剂科;遵义医学院药学院;
【基金】：贵州省教育厅自然科学基金资助项目[黔教合K字(2012)054号] 贵州省科学技术基金资助项目[黔科合J字(2008)215号]
【分类号】：R285.5;R-332
【参考文献】
相关期刊论文 前8条
1 彭定伟,刘丹,齐刚;利胆中药的研究进展[J];武警医学院学报;2005年01期
2 章学林;梁晓强;顾宏刚;马恩伟;林天碧;孙逊;张静U，
本文编号：2262331
本文链接：https://www.wllwen.com/yixuelunwen/shiyanyixue/2262331.html
最近更新
教材专著
