外周血调节性T淋巴细胞及Foxp3的表达与反复体外受精—胚胎移植失败的相关性研究
发布时间：2018-10-17 16:18
【摘要】： 目的1探讨外周血中CD4~+CD25~+调节性T细胞与反复体外受精-胚胎移植失败之间的相关性;2探讨外周血中Foxp3蛋白与mRNA的表达与反复体外受精-胚胎移植失败之间的相关性。 方法收取原因不明反复体外受精-胚胎移植失败者、正常非孕妇女及第一周期试管助孕成功者的外周静脉血(肝素钠抗凝)。用双荧光标记流式细胞分析技术,检测上述三组妇女外周血中CD4~+CD25~+T淋巴细胞及CD4~+CD25~(high)T淋巴细胞的表达情况;用免疫组化的方法检测三组妇女外周血中Foxp3蛋白的表达情况;用RT-PCR的方法检测三组妇女外周血中Foxp3 mRNA的表达。 结果1原因不明反复体外受精-胚胎移植失败患者外周血中CD4~+CD25~+T淋巴细胞及CD4~+CD25~(high)T淋巴细胞的表达均低于正常非孕妇女和第一周期试管助孕成功者;第一周期试管助孕成功者外周血中CD4~+CD25~+T淋巴细胞的表达低于正常非孕妇女,但CD4~+CD25~(high)T淋巴细胞的表达与正常非孕妇女无统计学差异;2原因不明反复体外受精-胚胎移植失败患者外周血中Foxp3蛋白的表达低于正常非孕妇女和第一周期试管助孕成功者,第一周期试管助孕成功者外周血中Foxp3蛋白的表达与正常非孕妇女无统计学差异;3原因不明反复体外受精-胚胎移植失败患者外周血中Foxp3 mRNA的表达低于正常非孕妇女和第一周期试管助孕成功者,第一周期试管助孕成功者外周血中Foxp3 mRNA的表达与正常非孕妇女无统计学差异。 结论外周血CD4~+CD25~+调节性T细胞尤其是CD4~+CD25~(high)调节性T淋巴细胞及FOXP3的表达异常可能与原因不明反复体外受精-胚胎移植失败的发生有相关性。
[Abstract]:Objective 1 To investigate the correlation between CD4 ~ + CD25 ~ + regulatory T cells and repeat in vitro fertilization-embryo transfer failure in peripheral blood, and to explore the correlation between the expression of Foxp3 protein and mRNA in peripheral blood and the failure of repeated in vitro fertilization-embryo transfer. Methods Peripheral venous blood (heparin) was collected from patients with unexplained recurrent in vitro fertilization-embryo transfer failure, normal non-pregnant women and first-cycle test tube assisted pregnancy. The expression of CD4 ~ + CD25 ~ + T lymphocytes and CD4 ~ + CD25 ~ (high) T lymphocytes in peripheral blood of three groups of women was detected by double fluorescence labeling flow cytometry. Expression of Foxp3 mRN in peripheral blood of three groups of women by RT-PCR Results 1 The expression of CD4 ~ + CD25 ~ + T lymphocytes and CD4 ~ + CD25 ~ (high) T lymphocytes in peripheral blood of patients with unexplained recurrent in vitro fertilization-embryo transplantation were lower than that of non-pregnant women and first. The expression of CD4 ~ + CD25 ~ + T lymphocytes in peripheral blood of the first cycle test tube was lower than that of non-pregnant women, but the expression of CD4 ~ + CD25 ~ (high) T lymphocytes was normal. The expression of Foxp3 protein in peripheral blood of patients with unexplained recurrent in vitro fertilization-embryo transfer failure was lower than that in non-pregnant women and the first cycle test tube, and the expression of Foxp3 protein in peripheral blood of the first cycle test tube was normal. The expression of Foxp3 mRNA in peripheral blood of patients with unexplained recurrent in vitro fertilization-embryo transfer failure was lower than that of non-pregnant women and the first cycle test tube, and the expression of Foxp3 mRNA in peripheral blood of the first cycle test tube was normal. Conclusion The abnormal expression of CD4 ~ + CD25 ~ + regulatory T cells, especially CD4 ~ + CD25 ~ (high) regulatory T lymphocytes and FOXP3 in peripheral blood may be associated with unexplained recurrent in vitro fertilization.
【学位授予单位】：安徽医科大学
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：R392
本文编号：2277229
[Abstract]:Objective 1 To investigate the correlation between CD4 ~ + CD25 ~ + regulatory T cells and repeat in vitro fertilization-embryo transfer failure in peripheral blood, and to explore the correlation between the expression of Foxp3 protein and mRNA in peripheral blood and the failure of repeated in vitro fertilization-embryo transfer. Methods Peripheral venous blood (heparin) was collected from patients with unexplained recurrent in vitro fertilization-embryo transfer failure, normal non-pregnant women and first-cycle test tube assisted pregnancy. The expression of CD4 ~ + CD25 ~ + T lymphocytes and CD4 ~ + CD25 ~ (high) T lymphocytes in peripheral blood of three groups of women was detected by double fluorescence labeling flow cytometry. Expression of Foxp3 mRN in peripheral blood of three groups of women by RT-PCR Results 1 The expression of CD4 ~ + CD25 ~ + T lymphocytes and CD4 ~ + CD25 ~ (high) T lymphocytes in peripheral blood of patients with unexplained recurrent in vitro fertilization-embryo transplantation were lower than that of non-pregnant women and first. The expression of CD4 ~ + CD25 ~ + T lymphocytes in peripheral blood of the first cycle test tube was lower than that of non-pregnant women, but the expression of CD4 ~ + CD25 ~ (high) T lymphocytes was normal. The expression of Foxp3 protein in peripheral blood of patients with unexplained recurrent in vitro fertilization-embryo transfer failure was lower than that in non-pregnant women and the first cycle test tube, and the expression of Foxp3 protein in peripheral blood of the first cycle test tube was normal. The expression of Foxp3 mRNA in peripheral blood of patients with unexplained recurrent in vitro fertilization-embryo transfer failure was lower than that of non-pregnant women and the first cycle test tube, and the expression of Foxp3 mRNA in peripheral blood of the first cycle test tube was normal. Conclusion The abnormal expression of CD4 ~ + CD25 ~ + regulatory T cells, especially CD4 ~ + CD25 ~ (high) regulatory T lymphocytes and FOXP3 in peripheral blood may be associated with unexplained recurrent in vitro fertilization.
【学位授予单位】：安徽医科大学
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：R392
【引证文献】
相关期刊论文 前1条
1 丁瑞芳;桂玉臻;丁国建;;160例孕妇保胎治疗相关因素分析[J];中国保健营养;2012年12期
，本文编号：2277229
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