基于P38MAPK探讨隔药饼灸对慢性支气管炎模型大鼠肺组织TNF-α的影响及作用机理

发布时间：2018-10-20 14:46

【摘要】：目的：观察隔药饼灸对慢性支气管炎大鼠肺组织中TNF-α、P-ATF2的影响,探讨其作用机制,为该法防治慢支提供实验依据。方法：将48只SPF级健康SD大鼠随机分为A(空白对照组)、B(慢支模型组)、C(隔药饼灸组)、D(隔纸垫灸组)四组,每组12只。采用改良烟熏法复制慢支大鼠模型。以隔药饼灸为处理因素,与空白组、模型组、隔纸垫灸组进行对照,取材分两次进行(即治疗前与治疗后),测定每组大鼠支气管肺泡灌洗液中(BALF)中巨噬细胞含量及肺组织中TNF-α、P-ATF2的含量变化,探讨隔药饼灸对P38MAPK调控CB大鼠免疫功能的影响。结果：(1)大鼠肺组织病理学变化：隔药饼灸和隔纸垫灸都有控制炎症的作用,隔药饼灸效果更明显。(2)大鼠BALF中细胞计数及AM(肺泡巨噬细胞)分类计数：隔药饼灸和隔纸垫灸能降低慢支大鼠BALF细胞总数及AM计数(P0.05)；隔药饼灸效果更好(P0.05)。(3)大鼠肺组织P-ATF2表达：隔药饼灸和隔纸垫灸均能降低慢支大鼠肺组织P-ATF2含量(P0.05)；隔药饼灸组效果更明显(P0.05)。(4)大鼠肺组织TNF-α mRNA及TNF-α蛋白表达：隔药饼灸和隔纸垫灸均能降低慢支大鼠肺组织TNF-α mRNA及TNF-α蛋白表达(P0.05)；隔药饼灸效果更好(P0.05)。(5)治疗前大鼠肺组织P-ATF2表达分别与TNF-αmRNA、TNF-α蛋白表达都呈正相关(r=0.597, r=0.642, P0.01)；治疗后大鼠肺组织P-ATF2表达分别与TNF-αmRNA、TNF-α蛋白表达都呈正相关(r=0.682, r=0.763, P＜0.01)。结论：(1)隔药饼灸可以降低CB大鼠BALF中AM含量,减轻炎症反应。(2)隔药饼灸能降低大鼠肺组织中TNF-α及P-ATF2含量。且P-ATF2在慢支的炎症反应中起着重要作用,隔药饼灸通过降低P-ATF2含量而减少其对TNF-α的激活,从而改善慢支炎症。(3)隔药饼灸和隔纸垫灸都能改善慢支大鼠的症状,但隔药饼灸效果更好。
[Abstract]:Objective: to observe the effect of herbal cake moxibustion on TNF- 伪 and P-ATF2 in lung tissue of rats with chronic bronchitis, to explore its mechanism, and to provide experimental basis for the prevention and treatment of chronic bronchitis by this method. Methods: Forty-eight healthy SD rats of SPF grade were randomly divided into four groups: group A (control group,), B (slow branch model group), C (separated cake moxibustion group,), D (paper-separated moxibustion group, 12 rats in each group. The rat model of chronic bronchitis was established by modified smoking method. Taking medicine-separated cake moxibustion as treatment factor, it was compared with blank group, model group and paper-separated moxibustion group. The contents of macrophages in bronchoalveolar lavage fluid (BALF) and changes of TNF- 伪 and P-ATF2 in lung tissue were measured in each group. The effect of moxibustion on the immune function of CB rats was studied. Results: (1) pathological changes of lung tissue in rats: both medicine-separated moxibustion and paper-separated moxibustion could control inflammation. (2) the cell count and AM (alveolar macrophage) count in rat BALF: moxibustion and paper-separated moxibustion could reduce the BALF cell count and AM count in slow branch rats (P0.05); The effect of medicine-separated cake moxibustion was better (P0.05). (3) the expression of P-ATF2 in lung tissue of rats was better than that of the control group (P0.05). The content of P-ATF2 in lung tissue of rats with slow bronchitis was decreased by the moxibustion of separated medicine cake and paper-separated moxibustion (P0.05). The expression of TNF- 伪 mRNA and TNF- 伪 protein in lung tissue was more obvious in the moxibustion group (P0.05). (4). The expression of TNF- 伪 mRNA and TNF- 伪 protein in the lung tissue of the chronic bronchitis rats was decreased by the moxibustion with the separated medicine cake and the paper-separated moxibustion (P0.05). The effect of moxibustion with medicine separated cake was better (P0.05). (5) the expression of P-ATF2 in lung tissue was positively correlated with the expression of TNF- 伪 mRNA,TNF- 伪 protein before treatment (r 0.597,0.642, P0.01), and the expression of P-ATF2 in lung tissue was positively correlated with the expression of TNF- 伪 mRNA,TNF- 伪 protein after treatment (r 0.682, r 0.763, P < 0.01). Conclusion: (1) moxibustion with medicine-separated cake can decrease the content of AM in BALF of CB rats and alleviate the inflammatory reaction. (2) moxibustion with separated medicine cake can decrease the content of TNF- 伪 and P-ATF2 in lung tissue of rats. P-ATF2 plays an important role in the inflammatory reaction of the slow bronchitis, and the activation of TNF- 伪 is reduced by decreasing the content of P-ATF2. (3) both the moxibustion of separated medicine cake and the moxibustion with paper partition can improve the symptoms of the rats with chronic bronchitis. But the effect of moxibustion with separated medicine cake is better.
【学位授予单位】：湖南中医药大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R245.82;R256.1;R-332

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