弓形虫入侵的宿主细胞骨架重塑触发线粒体重新分布

发布时间：2018-10-20 20:19

【摘要】：目的探讨宿主细胞骨架重塑在弓形虫侵入HFF细胞以及触发细胞线粒体重新分布中的作用。方法体外培养HFF细胞,预先用1μg/mL细胞松驰素D(CD)处理30min,接种弓形虫速殖子分别培养1h和20h,Western blotting检测弓形虫表面抗原(surface antigen,SAG)1蛋白表达。同时用MitoTracker汶Red CMXRos荧光探针标记细胞线粒体,激光共聚焦显微镜下观察HFF细胞线粒体在弓形虫侵入前后和CD处理前后聚集和分布情况。结果感染后1h,CD处理组HFF细胞内虫体量与CD未处理组差异不大,20h时CD未处理组细胞内虫体量显著多于CD处理组。此时可见HFF细胞线粒体明显聚集成明亮点状且分布于纳虫泡周围,而未感染组细胞线粒体未见明显聚集分布。CD可以显著抑制弓形虫侵入HFF细胞后引起的线粒体聚集。结论触发宿主细胞骨架重塑是弓形虫侵入宿主细胞并引起细胞线粒体向纳虫泡聚集所必须的。
[Abstract]:Objective to investigate the role of host cytoskeleton remodeling in Toxoplasma gondii invading HFF cells and triggering mitochondrial redistribution. Methods HFF cells were cultured in vitro and treated with 1 渭 g/mL D (CD) for 30 min. Toxoplasma gondii Tachyzoites were cultured for 1 h and 20 h, respectively. The expression of surface antigen,SAG 1 protein was detected by Western blotting. At the same time, the mitochondria of HFF cells were labeled with MitoTracker Wen Red CMXRos fluorescence probe. The aggregation and distribution of mitochondria in HFF cells before and after Toxoplasma gondii invasion and CD treatment were observed under confocal laser microscope. Results there was no significant difference between the HFF cells and the CD untreated group at 1 h after infection, but at 20 h after infection, the CD untreated group was significantly larger than the CD treatment group. At this time, the mitochondria of HFF cells were obviously aggregated into bright spots and distributed around the nardia vesicles, but not in the uninfected group. CD could significantly inhibit the mitochondrial aggregation induced by Toxoplasma gondii invading HFF cells. Conclusion it is necessary for Toxoplasma gondii to invade the host cells and cause mitochondria to accumulate into the NAA vesicles by triggering the cytoskeleton remodeling of host cells.
【作者单位】：中国疾病预防控制中心寄生虫病预防控制所;江苏大学医学院;
【基金】：国家自然科学基金(No.81301453) 卫生部寄生虫病原与媒介生物学重点实验室开放课题(No.WSBKTKT201302) 中国博士后科学基金特别资助(No.2015T80518);中国博士后科学基金(No.2014M561598) 江苏省博士后科研计划(No.1402171C) 江苏大学高级人才启动基金(No.13JDG024,No.13JDG127)联合资助~~
【分类号】：R382.5

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