不同程度脊髓背侧压迫损伤模型大鼠脊髓损伤致伤器的设计

发布时间：2018-10-21 18:33

【摘要】：背景:随着对脊髓损伤研究的不断深入,国内外学者不断设计出各种脊髓损伤模型,主要包括脊髓挫伤、重物坠击、脊髓压迫、化学灼伤、放射性损伤及激素横断半横断损伤等,然而各种制备方法都存在一定的局限。目的:设计脊髓损伤致伤器并建立不同程度脊髓损伤动物模型。方法:实验自行设计包括致伤部件及传动装置的脊髓损伤致伤器,利用不同致伤质量m1=10 g,m2=20 g,m3=30 g及时间T1=3 s,T2=5 s,造成不同质量×时间暨不同程度SD大鼠脊髓背侧压迫损伤,分为m1T1,m2T1,m3T1,m1T2,m2T2,m3T2共6组,并设假手术组进行对照。结果与结论:造模后各实验组BBB评分均低于假手术组(P0.01),m1T1组与其他各实验组相比差异有显著性意义(P0.01),建模后8周m1T2组BBB评分高于与m2T2组及m3T2组(P0.05)。建模后8周各组体感诱发电位和运动诱发电位潜伏期明显长于假手术组(P0.01)。除了m1T1组与m1T2组外,其他各组间建模后体感诱发电位潜伏期均明显延长(P0.05)。所有组建模后运动诱发电位潜伏期均明显延长(P0.05)。结果证实,实验所设计的脊髓损伤致伤器可制备出不同程度的脊髓背侧压迫损伤动物模型。
[Abstract]:Background: with the development of the research on spinal cord injury, scholars at home and abroad have designed various spinal cord injury models, including spinal cord contusion, heavy weight drop, spinal cord compression, chemical burns. However, there are some limitations in various preparation methods, such as radiation injury and hormone transection injury. Objective: to design a spinal cord injury apparatus and to establish animal models of spinal cord injury. Methods: the spinal cord injury apparatus including injured parts and transmission device was designed by ourselves. The spinal cord injury was induced by using different injury mass of 10 g / m ~ (2) and 20 g / m ~ (3) C ~ (30 g) and time T _ (1) ~ (3) S ~ (3) T _ (2) + T _ (2) ~ (5) s to induce spinal cord dorsal compression injury in different quality 脳 time and different degree of SD rats. The patients were divided into 6 groups: m1T1, m2T1, m3T1, m1T2, m2T2m2m3T2, and sham-operated group. Results and conclusion: the BBB score of each experimental group was lower than that of sham operation group (P0.01), and the BBB score of m1T2 group was higher than that of m2T2 group and m3T2 group at 8 weeks after modeling (P0.01), and there was significant difference between m1T1 group and other experimental groups (P0.01). The latency of somatosensory evoked potential and motor evoked potential in each group was significantly longer than that in sham operation group at 8 weeks after modeling (P0.01). The latency of somatosensory evoked potential (SEP) was significantly prolonged after modeling between m1T1 group and m1T2 group (P0.05). After modeling, the latency of motor evoked potential was significantly prolonged in all groups (P0.05). The results show that the spinal cord injury apparatus designed by the experiment can produce the animal model of spinal cord dorsal compression injury with different degrees.
【作者单位】：天津医科大学总医院骨科;内蒙古医科大学第二附属医院康复科;
【分类号】：R651.2;R-332

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