新生鼠和胎鼠海马神经干细胞分离和培养方法的比较
发布时间：2018-10-29 20:01
【摘要】：目的:探索大鼠海马神经干细胞离体培养的实验条件和方法,并建立一种简便且获得率较高的实验方法。方法:分别随机选取新生1~3 d SD大鼠和孕18~19 d SD胎鼠,并提取海马组织,采用机械吹打结合胰蛋白酶消化的方法获得单细胞悬液,然后对培养出的细胞进行鉴定和诱导分化,最后测定并分别比较两组原代细胞获得率和增殖率以及凋亡率之间的差异。结果:来源于两种不同发育阶段大鼠的海马组织培养出的细胞均呈Nestin阳性和Brd U阳性的神经球样结构,诱导分化后的细胞高表达βⅢ-tublin和GFAP。其中,胎鼠组原代细胞获得率较高、增殖能力较强,并有统计学差异(P0.05);但两组神经干细胞的凋亡率无明显差异(P0.05)。结论:胎鼠和新生鼠海马组织均能培养出神经干细胞,而且胎鼠组细胞产出率更高。
[Abstract]:Aim: to explore the experimental conditions and methods of rat hippocampal neural stem cells (NSCs) culture in vitro and to establish a simple and efficient method. Methods: single cell suspension was obtained by mechanical blow and trypsin digestion from hippocampal tissue of neonatal SD rats and 1819 SD fetal rats. The cultured cells were identified and induced to differentiate. Finally, the difference between the primary cell acquisition rate, proliferation rate and apoptosis rate was measured and compared between the two groups. Results: the cultured cells from the hippocampal tissues of two different developmental stages showed Nestin positive and Brd U positive neuron-like structures, and the differentiated cells expressed high 尾 鈪，
本文编号：2298669
[Abstract]:Aim: to explore the experimental conditions and methods of rat hippocampal neural stem cells (NSCs) culture in vitro and to establish a simple and efficient method. Methods: single cell suspension was obtained by mechanical blow and trypsin digestion from hippocampal tissue of neonatal SD rats and 1819 SD fetal rats. The cultured cells were identified and induced to differentiate. Finally, the difference between the primary cell acquisition rate, proliferation rate and apoptosis rate was measured and compared between the two groups. Results: the cultured cells from the hippocampal tissues of two different developmental stages showed Nestin positive and Brd U positive neuron-like structures, and the differentiated cells expressed high 尾 鈪，
本文编号：2298669
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