小白菊内酯对帕金森病MPTP模型小鼠抗炎作用的研究

发布时间：2018-11-01 13:23

【摘要】：目的研究艾菊有效成分小白菊内酯在1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)所致小鼠帕金森病(PD)模型中对炎症因子环氧合酶-2(COX-2)及其产物前列腺素E2(PGE2)以及一氧化氮合酶(iNOS)表达调控的影响以及对多巴胺能神经元的保护作用。方法应用MPTP制备亚急性PD小鼠模型,观察小鼠行为学变化,采用免疫组织化学和免疫蛋白印记法观察小鼠黑质酪氨酸羟化酶(TH),COX-2、PGE2以及iNOS的表达变化过程;并观察给予小白菊内酯后对上述变化的影响。结果与对照组相比,模型组小鼠出现典型PD症状,COX-2、PGE2、i NOS阳性细胞显著增加(P0.01),同时伴有TH阳性神经元显著丢失58%(P0.01);给予小白菊内酯处理后,模型小鼠PD症状减轻,黑质区COX-2、PGE2、iNOS阳性细胞明显减少(P0.01),TH阳性细胞数较对照组仅下降27%。结论小白菊内酯可影响MPTP模型小鼠黑质COX-2、PGE2、iNOS的表达,起到抗炎作用进而对小鼠多巴胺能神经元起到一定保护作用。
[Abstract]:Objective to study the effect of albinolactone on 1-methyl-4-phenyl-1m-2, an effective component of Artemisia officinalis. The effects of (MPTP) on the expression of inflammatory factor cyclooxygenase-2 (COX-2) and its product prostaglandin E _ 2 (PGE2) and nitric oxide synthase (iNOS) in (PD) model of Parkinson's disease in mice And its protective effect on dopaminergic neurons. Methods Subacute PD mice model was made by MPTP and the behavior of mice was observed. The expression of tyrosine hydroxylase (TH), COX-2,PGE2 and iNOS in substantia nigra was observed by immunohistochemistry and immuno protein imprinting. The effects of albendron lactone on the above changes were observed. Results compared with the control group, the model group showed typical PD symptoms, COX-2,PGE2,i NOS positive cells increased significantly (P0.01), and TH positive neurons were significantly lost in 58% (P0.01). The symptoms of PD in model mice were alleviated and the number of COX-2,PGE2,iNOS positive cells in substantia nigra decreased significantly (P0.01), TH positive cells decreased only 27% as compared with control group). Conclusion the expression of COX-2,PGE2,iNOS in the substantia nigra of MPTP model mice can be affected by small chrysanthemum lactone, which can protect dopaminergic neurons from inflammation and play a certain role in protecting dopaminergic neurons.
【作者单位】：唐山市第二医院;河北联合大学基础医学院;
【基金】：河北省科学技术与社会发展计划项目(04276135) 唐山市科学技术研究与发展计划指令项目(13130267z) 河北联合大学科学研究基金(Z201236) 2013年河北省大学生创新创业训练计划项目(201310081023) 河北联合大学2013年大学生创新性实验计划(X2013034)
【分类号】：R-332;R285.5

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