HPLC分析植物乳杆菌胆盐水解酶降解特性

发布时间：2018-11-05 13:00

【摘要】：本文建立了同时检测6种胆盐的HPLC方法,并对两株植物乳杆菌来源的胆盐水解酶BSH-1降解特性进行了分析。HPLC色谱条件如下:Sepax Bio-C18(3μm,4.6 mm×150 mm);流速:0.6 m L/min;检测波长:205 nm;流动相A:水/TFA=100/0.1,流动相B:乙腈/TFA=100/0.09;洗脱梯度如下:0 min~8 min,B 25%~35%;8 min~30 min,B 35%~48%;30 min~30.5 min,B48%~25%;30.5 min~40 min,B 25%。结果表明,本文建立的HPLC法可以快速准确的测定BSH-1对胆盐的降解活力及偏好,有利于筛选具有高效降解胆盐的乳酸菌。两种植物乳杆菌来源的BSH-1对GC、GCDC、GDC这三种胆盐可以进行降解,尤其是GDC的降解率高达99.9%,但是对TAC、TCDC、TDC几乎不能降解,也即是来源于植物乳杆菌的BSH-1对降解甘氨系列胆酸盐的能力是高于牛磺系列胆酸盐。
[Abstract]:In this paper, a HPLC method for simultaneous detection of 6 kinds of bile salts was established, and the degradation characteristics of bile salt hydrolase BSH-1 from two strains of Lactobacillus plantarum were analyzed. The chromatographic conditions of HPLC were as follows: Sepax Bio-C18 (3 渭 m) 4.6 mm 脳 150 mm); The flow rate was 0. 6 mL / min, the detection wavelength was 205 nm; mobile phase A: water / TFA=100/0.1, mobile phase B: acetonitrile / TFA=100/0.09; elution gradient was as follows: 0 min~8 min,B 25 + 35 + 8 min~30 min,B 35. 30 min~30.5 min,B48%~25%;30.5 min~40 min,B 25. The results showed that the HPLC method established in this paper could quickly and accurately determine the degradation activity and preference of BSH-1 to bile salt, and was beneficial to the screening of lactic acid bacteria with high degradation of bile salt. BSH-1 from two strains of Lactobacillus plantarum can degrade GC,GCDC,GDC, especially the degradation rate of GDC as high as 99.9%, but it can hardly degrade TAC,TCDC,TDC. The ability of BSH-1 derived from Lactobacillus plantarum to degrade glargine series cholate is higher than that of taurocholate series.
【作者单位】：上海理工大学医疗器械与食品学院;
【基金】：国家自然科学基金资助项目(31371809) 上海科委国际合作项目(14390711700) 沪江基金研究基地专项(D15012)
【分类号】：R3411;O657.72

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