卡氏肺孢子虫生长发育的观察研究
发布时间：2018-11-07 12:06
【摘要】： 目的观察Wistar大鼠肺组织中卡氏肺孢子虫的生长发育和形态结构。 方法通过皮下注射地塞米松磷酸钠建立Wistar大鼠卡氏肺孢子虫肺炎动物模型为实验组,另设不注射药物为对照组。制备大鼠肺印片标本,瑞-姬氏染色,光学显微镜下检测和观察卡氏肺孢子虫。取大鼠肺组织标本,制备肺组织切片和透射、扫描电镜观察标本,分别使用光镜、透射和扫描电镜观察该虫生长发育、形态结构及其感染大鼠肺病理学变化。用数字图像分析技术,对卡氏肺孢子虫包囊进行形态学参数测定。 结果肺印片标本经瑞-姬氏染色,光学显微镜油镜下检测,可见清晰卡氏肺孢子虫包囊和滋养体,以肺泡内多见,偶可在细胞内见到。对照组大鼠未检测到该虫。实验组大鼠肺印片标本该虫检出率为57%,第4～5周查到包囊为主,7～8周后以滋养体为主。扫描电镜观察,卡氏肺孢子虫有表面光滑和粗糙两种类型,其中粗糙型又有微绒毛、管状结构、颗粒样物三种情况;观察到壁上有凹陷、正在脱囊及伸出伪足的虫体。透射电镜观察到卡氏肺孢子虫有滋养体、囊前期和包囊三个发育阶段。滋养体多附着于肺Ⅰ型上皮细胞,偶见附着于肺Ⅱ型上皮细胞,有的伸出1或多个较宽大伪足,部分细胞质内发现核相关细胞器和纺锤微管。囊前期有早、中、晚三种类型,在其内发现有代表减数分裂的联会复合体。包囊壁上有一增厚处,内有一孔隙。第10周实验组大鼠肺间隔及气管中可见到卡氏肺孢子虫。用数字图像处理技术测量卡氏肺孢子虫包囊长径、短径、投影面积、体积、周长、等效直径和圆球度数值均数分别为:4.8159μm,3.6923μm,13.07μm~2,36.76μm~3,14.29μm,4.03μm和0.8。光镜观察HE染色的肺组织切片,第7～8周实验组大鼠肺泡内被大量虫体和渗出物充满,肺Ⅱ型上皮细胞脱落,肺泡隔增厚。扫描电镜观察肺内有卡氏肺孢子虫,肺组织呈纤维性改变。透射电镜观察,肺Ⅱ型上皮细胞质内可见许多空泡和电子密度高的颗粒。含虫体的肺Ⅱ型上皮细胞表膜突出,形成质膜小泡,细胞核膜内陷,核内染色体呈丝状并绕成团。 结论瑞-姬氏复染法检测卡氏肺孢子虫,操作简单、虫体清晰,有利于一般实验室对该虫检测。扫描电镜观察到表面有粗细不等的管状物和正在脱囊的虫体,阳性大鼠肺组织纤维化改变,为国内首次报道。透射电镜观察到卡氏肺孢子虫的滋养体、囊前期和包囊三个发育阶段;滋养体附着于肺Ⅱ型上皮细胞及气管内,国内外未见报道;包囊壁上观察到一增厚处,内有一孔隙,为罕见报道。数字图像分析技术获得了卡氏肺孢子虫包囊的一系列数据,丰富了肺孢子虫生物学资料,为病原学诊断提供参考资料。
[Abstract]:Objective to observe the growth, development and morphology of Pneumocystis carinii in lung tissue of Wistar rats. Methods the animal model of pneumocystis carinii pneumonia was established by subcutaneous injection of dexamethasone sodium phosphate in Wistar rats. Rat lung impressions were prepared and stained by Riggi's staining. Pneumocystis carinii was detected and observed under optical microscope. Lung tissue sections and transmission were prepared from rat lung tissue. Light microscope, transmission electron microscope and scanning electron microscope were used to observe the growth and development, morphological structure and pathological changes of lung of infected rats. The morphological parameters of the cysts of Pneumocystis carinii were measured by digital image analysis. Results the pulmonary specimens were stained by Ricky's stain and detected by oil microscope. The clear cysts and trophozoites of Pneumocystis carinii were found in the alveoli and occasionally in the cells. The worm was not detected in the control group. In the experimental group, the positive rate of the worm was 57%, the cyst was found in the 4th week, and the trophozoite was the main one after 78 weeks. Scanning electron microscope (SEM) showed that Pneumocystis carinii had smooth surface and rough surface, including rough type and microvilli, tubular structure and granulocytes, and there were indentation in the wall, exfoliation and extension of pseudopodia on the wall of Pneumocystis carinii. Transmission electron microscopy showed that Pneumocystis carinii had trophozoites, precysts and cysts. Most trophozoites were attached to type I epithelial cells, and occasionally to type 鈪，
本文编号：2316317
[Abstract]:Objective to observe the growth, development and morphology of Pneumocystis carinii in lung tissue of Wistar rats. Methods the animal model of pneumocystis carinii pneumonia was established by subcutaneous injection of dexamethasone sodium phosphate in Wistar rats. Rat lung impressions were prepared and stained by Riggi's staining. Pneumocystis carinii was detected and observed under optical microscope. Lung tissue sections and transmission were prepared from rat lung tissue. Light microscope, transmission electron microscope and scanning electron microscope were used to observe the growth and development, morphological structure and pathological changes of lung of infected rats. The morphological parameters of the cysts of Pneumocystis carinii were measured by digital image analysis. Results the pulmonary specimens were stained by Ricky's stain and detected by oil microscope. The clear cysts and trophozoites of Pneumocystis carinii were found in the alveoli and occasionally in the cells. The worm was not detected in the control group. In the experimental group, the positive rate of the worm was 57%, the cyst was found in the 4th week, and the trophozoite was the main one after 78 weeks. Scanning electron microscope (SEM) showed that Pneumocystis carinii had smooth surface and rough surface, including rough type and microvilli, tubular structure and granulocytes, and there were indentation in the wall, exfoliation and extension of pseudopodia on the wall of Pneumocystis carinii. Transmission electron microscopy showed that Pneumocystis carinii had trophozoites, precysts and cysts. Most trophozoites were attached to type I epithelial cells, and occasionally to type 鈪，
本文编号：2316317
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